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Detection of SARS-CoV-2 Specific Antibodies in Saliva Samples
Molecular assays on nasopharyngeal swabs act as a confirmatory test in coronavirus disease (COVID-19) diagnosis. However, the technical requirements of nasopharyngeal sampling and molecular assays limit the testing capabilities. Recent studies suggest the use of saliva for the COVID-19 diagnostic te...
Autores principales: | , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9309249/ https://www.ncbi.nlm.nih.gov/pubmed/35898491 http://dx.doi.org/10.3389/fimmu.2022.880154 |
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author | Yu, Siyang Zhang, Peiyan Liao, Mingfeng Zhang, Juanjuan Luo, Suisui Zhai, Jinglei Zhang, Yaxi Lin, Jingyan Yuan, Jing Zhang, Zheng Wang, Fuxiang Wei, Lanlan |
author_facet | Yu, Siyang Zhang, Peiyan Liao, Mingfeng Zhang, Juanjuan Luo, Suisui Zhai, Jinglei Zhang, Yaxi Lin, Jingyan Yuan, Jing Zhang, Zheng Wang, Fuxiang Wei, Lanlan |
author_sort | Yu, Siyang |
collection | PubMed |
description | Molecular assays on nasopharyngeal swabs act as a confirmatory test in coronavirus disease (COVID-19) diagnosis. However, the technical requirements of nasopharyngeal sampling and molecular assays limit the testing capabilities. Recent studies suggest the use of saliva for the COVID-19 diagnostic test. In this study, 44 patients diagnosed with COVID-19 in The Third People’s Hospital of Shenzhen were enrolled. Saliva and serum specimens were obtained at different time points and the immunoglobulins against SARS-CoV-2 were measured. The results showed that saliva IgA presented a higher COI value than IgG and IgM. In matched saliva and serum samples, all saliva samples presented lower IgG levels than serum samples, and only one saliva sample presented a higher IgM level. The conversion rates of saliva IgA and the detection of viral nucleic acids were analyzed in the first and second weeks after hospitalization. The positive rates increased when combining saliva IgA and viral nucleic acid detection. In conclusion, our results provide evidence that saliva IgA could serve as a useful index for the early diagnosis of COVID-19. |
format | Online Article Text |
id | pubmed-9309249 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-93092492022-07-26 Detection of SARS-CoV-2 Specific Antibodies in Saliva Samples Yu, Siyang Zhang, Peiyan Liao, Mingfeng Zhang, Juanjuan Luo, Suisui Zhai, Jinglei Zhang, Yaxi Lin, Jingyan Yuan, Jing Zhang, Zheng Wang, Fuxiang Wei, Lanlan Front Immunol Immunology Molecular assays on nasopharyngeal swabs act as a confirmatory test in coronavirus disease (COVID-19) diagnosis. However, the technical requirements of nasopharyngeal sampling and molecular assays limit the testing capabilities. Recent studies suggest the use of saliva for the COVID-19 diagnostic test. In this study, 44 patients diagnosed with COVID-19 in The Third People’s Hospital of Shenzhen were enrolled. Saliva and serum specimens were obtained at different time points and the immunoglobulins against SARS-CoV-2 were measured. The results showed that saliva IgA presented a higher COI value than IgG and IgM. In matched saliva and serum samples, all saliva samples presented lower IgG levels than serum samples, and only one saliva sample presented a higher IgM level. The conversion rates of saliva IgA and the detection of viral nucleic acids were analyzed in the first and second weeks after hospitalization. The positive rates increased when combining saliva IgA and viral nucleic acid detection. In conclusion, our results provide evidence that saliva IgA could serve as a useful index for the early diagnosis of COVID-19. Frontiers Media S.A. 2022-07-08 /pmc/articles/PMC9309249/ /pubmed/35898491 http://dx.doi.org/10.3389/fimmu.2022.880154 Text en Copyright © 2022 Yu, Zhang, Liao, Zhang, Luo, Zhai, Zhang, Lin, Yuan, Zhang, Wang and Wei https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Immunology Yu, Siyang Zhang, Peiyan Liao, Mingfeng Zhang, Juanjuan Luo, Suisui Zhai, Jinglei Zhang, Yaxi Lin, Jingyan Yuan, Jing Zhang, Zheng Wang, Fuxiang Wei, Lanlan Detection of SARS-CoV-2 Specific Antibodies in Saliva Samples |
title | Detection of SARS-CoV-2 Specific Antibodies in Saliva Samples |
title_full | Detection of SARS-CoV-2 Specific Antibodies in Saliva Samples |
title_fullStr | Detection of SARS-CoV-2 Specific Antibodies in Saliva Samples |
title_full_unstemmed | Detection of SARS-CoV-2 Specific Antibodies in Saliva Samples |
title_short | Detection of SARS-CoV-2 Specific Antibodies in Saliva Samples |
title_sort | detection of sars-cov-2 specific antibodies in saliva samples |
topic | Immunology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9309249/ https://www.ncbi.nlm.nih.gov/pubmed/35898491 http://dx.doi.org/10.3389/fimmu.2022.880154 |
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