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Effect of pH on Diclofenac–Lysozyme Interaction: Structural and Functional Aspect

As a nonsteroidal antiinflammatory drug, diclofenac (DCF) is used in the treatment of a variety of human ailments. It has already been reported that the use of this class of drugs for a longer duration is associated with numerous side effects such as cardiovascular implications, reno-medullary compl...

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Autores principales: Basheeruddin, Mohd, Khan, Sheeza, Ahmed, Neesar, Jamal, Shazia
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9309515/
https://www.ncbi.nlm.nih.gov/pubmed/35898307
http://dx.doi.org/10.3389/fmolb.2022.872905
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author Basheeruddin, Mohd
Khan, Sheeza
Ahmed, Neesar
Jamal, Shazia
author_facet Basheeruddin, Mohd
Khan, Sheeza
Ahmed, Neesar
Jamal, Shazia
author_sort Basheeruddin, Mohd
collection PubMed
description As a nonsteroidal antiinflammatory drug, diclofenac (DCF) is used in the treatment of a variety of human ailments. It has already been reported that the use of this class of drugs for a longer duration is associated with numerous side effects such as cardiovascular implications, reno-medullary complications, etc. In the present study, the effect of DCF on the structure, stability, and function of lysozyme was studied. The study was designed to examine the effect of DCF only at various pH values. Heat-induced denaturation of lysozyme was analyzed in the presence and absence of various molar concentrations of DCF at different pH values. The values of thermodynamic parameters, the midpoint of denaturation (T (m)), enthalpy change at T (m) (ΔH (m)), constant pressure heat capacity change (ΔC (p)), and Gibbs energy change at 25°C (ΔG (D) (o)), thus obtained under a given set of conditions (pH and molar concentration of DCF), demonstrated the following 1) DCF destabilized lysozyme with respect of T (m) and ΔG (D) (o) at all the pH values, 2) the magnitude of protein destabilization is lesser at acidic pH than at physiological pH, 3) structural changes in lysozyme are less projecting at pH 2.0 than at pH 7.0, and 4) quenching is observed at both pH values. Furthermore, the process of protein destabilization in the presence of DCF is entropically driven.
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spelling pubmed-93095152022-07-26 Effect of pH on Diclofenac–Lysozyme Interaction: Structural and Functional Aspect Basheeruddin, Mohd Khan, Sheeza Ahmed, Neesar Jamal, Shazia Front Mol Biosci Molecular Biosciences As a nonsteroidal antiinflammatory drug, diclofenac (DCF) is used in the treatment of a variety of human ailments. It has already been reported that the use of this class of drugs for a longer duration is associated with numerous side effects such as cardiovascular implications, reno-medullary complications, etc. In the present study, the effect of DCF on the structure, stability, and function of lysozyme was studied. The study was designed to examine the effect of DCF only at various pH values. Heat-induced denaturation of lysozyme was analyzed in the presence and absence of various molar concentrations of DCF at different pH values. The values of thermodynamic parameters, the midpoint of denaturation (T (m)), enthalpy change at T (m) (ΔH (m)), constant pressure heat capacity change (ΔC (p)), and Gibbs energy change at 25°C (ΔG (D) (o)), thus obtained under a given set of conditions (pH and molar concentration of DCF), demonstrated the following 1) DCF destabilized lysozyme with respect of T (m) and ΔG (D) (o) at all the pH values, 2) the magnitude of protein destabilization is lesser at acidic pH than at physiological pH, 3) structural changes in lysozyme are less projecting at pH 2.0 than at pH 7.0, and 4) quenching is observed at both pH values. Furthermore, the process of protein destabilization in the presence of DCF is entropically driven. Frontiers Media S.A. 2022-07-11 /pmc/articles/PMC9309515/ /pubmed/35898307 http://dx.doi.org/10.3389/fmolb.2022.872905 Text en Copyright © 2022 Basheeruddin, Khan, Ahmed and Jamal. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Molecular Biosciences
Basheeruddin, Mohd
Khan, Sheeza
Ahmed, Neesar
Jamal, Shazia
Effect of pH on Diclofenac–Lysozyme Interaction: Structural and Functional Aspect
title Effect of pH on Diclofenac–Lysozyme Interaction: Structural and Functional Aspect
title_full Effect of pH on Diclofenac–Lysozyme Interaction: Structural and Functional Aspect
title_fullStr Effect of pH on Diclofenac–Lysozyme Interaction: Structural and Functional Aspect
title_full_unstemmed Effect of pH on Diclofenac–Lysozyme Interaction: Structural and Functional Aspect
title_short Effect of pH on Diclofenac–Lysozyme Interaction: Structural and Functional Aspect
title_sort effect of ph on diclofenac–lysozyme interaction: structural and functional aspect
topic Molecular Biosciences
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9309515/
https://www.ncbi.nlm.nih.gov/pubmed/35898307
http://dx.doi.org/10.3389/fmolb.2022.872905
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