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Dairy Cows Experimentally Infected With Bovine Leukemia Virus Showed an Increased Milk Production in Lactation Numbers 3–4: A 4-Year Longitudinal Study

Bovine leukemia virus (BLV) is widespread in global cattle populations, but the effects of its infection on milk quantity and quality have not been clearly elucidated in animal models. In this study, 30 healthy first-lactation cows were selected from ≈2,988 cows in a BLV-free farm with the same crit...

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Autores principales: Yang, Yi, Gong, Zaicheng, Lu, Yi, Lu, Xubin, Zhang, Jilei, Meng, Ye, Peng, Yalan, Chu, Shuangfeng, Cao, Wenqiang, Hao, Xiaoli, Sun, Jie, Wang, Heng, Qin, Aijian, Wang, Chengming, Shang, Shaobin, Yang, Zhangping
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9309534/
https://www.ncbi.nlm.nih.gov/pubmed/35898913
http://dx.doi.org/10.3389/fmicb.2022.946463
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author Yang, Yi
Gong, Zaicheng
Lu, Yi
Lu, Xubin
Zhang, Jilei
Meng, Ye
Peng, Yalan
Chu, Shuangfeng
Cao, Wenqiang
Hao, Xiaoli
Sun, Jie
Wang, Heng
Qin, Aijian
Wang, Chengming
Shang, Shaobin
Yang, Zhangping
author_facet Yang, Yi
Gong, Zaicheng
Lu, Yi
Lu, Xubin
Zhang, Jilei
Meng, Ye
Peng, Yalan
Chu, Shuangfeng
Cao, Wenqiang
Hao, Xiaoli
Sun, Jie
Wang, Heng
Qin, Aijian
Wang, Chengming
Shang, Shaobin
Yang, Zhangping
author_sort Yang, Yi
collection PubMed
description Bovine leukemia virus (BLV) is widespread in global cattle populations, but the effects of its infection on milk quantity and quality have not been clearly elucidated in animal models. In this study, 30 healthy first-lactation cows were selected from ≈2,988 cows in a BLV-free farm with the same criteria of parity, age, lactation number, as well as milk yield, SCS, and composition (fat, protein, and lactose). Subsequently, these cows were randomly assigned to the intervention (n = 15) or control (n = 15) group, and reared in different cowsheds. Cows in the intervention group were inoculated with 1 × phosphate-buffered solution (PBS) resuspended in peripheral blood mononuclear cells (PBMC) from a BLV-positive cow, while the controls were inoculated with the inactivated PBMC from the same individual. From June 2016 to July 2021, milk weight (kg) was automatically recorded by milk sensors, and milk SCS and composition were originated from monthly performed dairy herd improvement (DHI) testing. Fluorescence resonance energy transfer (FRET)–qPCR and ELISA showed that cows in the intervention group were successfully infected with BLV, while cows in the control group were free of BLV for the entire period. At 45 days post-inoculation (DPI), the numbers of whole blood cells (WBCs) (P = 0.010), lymphocytes (LYMs) (P = 0.002), and monocytes (MNCs) (P = 0.001) and the expression levels of IFN-γ (P = 0.013), IL-10 (P = 0.031), and IL-12p70 (P = 0.008) increased significantly in the BLV infected cows compared to the non-infected. In lactation numbers 2–4, the intervention group had significantly higher overall milk yield (P < 0.001), fat (P = 0.031), and protein (P = 0.050) than the control group, while milk SCS (P = 0.038) and lactose (P = 0.036) decreased significantly. Further analysis indicated that BLV infection was associated with increased milk yield at each lactation stage in lactation numbers 3–4 (P = 0.021 or P < 0.001), but not with SCS and milk composition. Together, this 4-year longitudinal study revealed that artificial inoculation of BLV increased the milk yield in cows in this BLV challenge model.
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spelling pubmed-93095342022-07-26 Dairy Cows Experimentally Infected With Bovine Leukemia Virus Showed an Increased Milk Production in Lactation Numbers 3–4: A 4-Year Longitudinal Study Yang, Yi Gong, Zaicheng Lu, Yi Lu, Xubin Zhang, Jilei Meng, Ye Peng, Yalan Chu, Shuangfeng Cao, Wenqiang Hao, Xiaoli Sun, Jie Wang, Heng Qin, Aijian Wang, Chengming Shang, Shaobin Yang, Zhangping Front Microbiol Microbiology Bovine leukemia virus (BLV) is widespread in global cattle populations, but the effects of its infection on milk quantity and quality have not been clearly elucidated in animal models. In this study, 30 healthy first-lactation cows were selected from ≈2,988 cows in a BLV-free farm with the same criteria of parity, age, lactation number, as well as milk yield, SCS, and composition (fat, protein, and lactose). Subsequently, these cows were randomly assigned to the intervention (n = 15) or control (n = 15) group, and reared in different cowsheds. Cows in the intervention group were inoculated with 1 × phosphate-buffered solution (PBS) resuspended in peripheral blood mononuclear cells (PBMC) from a BLV-positive cow, while the controls were inoculated with the inactivated PBMC from the same individual. From June 2016 to July 2021, milk weight (kg) was automatically recorded by milk sensors, and milk SCS and composition were originated from monthly performed dairy herd improvement (DHI) testing. Fluorescence resonance energy transfer (FRET)–qPCR and ELISA showed that cows in the intervention group were successfully infected with BLV, while cows in the control group were free of BLV for the entire period. At 45 days post-inoculation (DPI), the numbers of whole blood cells (WBCs) (P = 0.010), lymphocytes (LYMs) (P = 0.002), and monocytes (MNCs) (P = 0.001) and the expression levels of IFN-γ (P = 0.013), IL-10 (P = 0.031), and IL-12p70 (P = 0.008) increased significantly in the BLV infected cows compared to the non-infected. In lactation numbers 2–4, the intervention group had significantly higher overall milk yield (P < 0.001), fat (P = 0.031), and protein (P = 0.050) than the control group, while milk SCS (P = 0.038) and lactose (P = 0.036) decreased significantly. Further analysis indicated that BLV infection was associated with increased milk yield at each lactation stage in lactation numbers 3–4 (P = 0.021 or P < 0.001), but not with SCS and milk composition. Together, this 4-year longitudinal study revealed that artificial inoculation of BLV increased the milk yield in cows in this BLV challenge model. Frontiers Media S.A. 2022-07-11 /pmc/articles/PMC9309534/ /pubmed/35898913 http://dx.doi.org/10.3389/fmicb.2022.946463 Text en Copyright © 2022 Yang, Gong, Lu, Lu, Zhang, Meng, Peng, Chu, Cao, Hao, Sun, Wang, Qin, Wang, Shang and Yang. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Yang, Yi
Gong, Zaicheng
Lu, Yi
Lu, Xubin
Zhang, Jilei
Meng, Ye
Peng, Yalan
Chu, Shuangfeng
Cao, Wenqiang
Hao, Xiaoli
Sun, Jie
Wang, Heng
Qin, Aijian
Wang, Chengming
Shang, Shaobin
Yang, Zhangping
Dairy Cows Experimentally Infected With Bovine Leukemia Virus Showed an Increased Milk Production in Lactation Numbers 3–4: A 4-Year Longitudinal Study
title Dairy Cows Experimentally Infected With Bovine Leukemia Virus Showed an Increased Milk Production in Lactation Numbers 3–4: A 4-Year Longitudinal Study
title_full Dairy Cows Experimentally Infected With Bovine Leukemia Virus Showed an Increased Milk Production in Lactation Numbers 3–4: A 4-Year Longitudinal Study
title_fullStr Dairy Cows Experimentally Infected With Bovine Leukemia Virus Showed an Increased Milk Production in Lactation Numbers 3–4: A 4-Year Longitudinal Study
title_full_unstemmed Dairy Cows Experimentally Infected With Bovine Leukemia Virus Showed an Increased Milk Production in Lactation Numbers 3–4: A 4-Year Longitudinal Study
title_short Dairy Cows Experimentally Infected With Bovine Leukemia Virus Showed an Increased Milk Production in Lactation Numbers 3–4: A 4-Year Longitudinal Study
title_sort dairy cows experimentally infected with bovine leukemia virus showed an increased milk production in lactation numbers 3–4: a 4-year longitudinal study
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9309534/
https://www.ncbi.nlm.nih.gov/pubmed/35898913
http://dx.doi.org/10.3389/fmicb.2022.946463
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