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Establishing Sterility Assurance for Bacillus canaveralius 29669 Spores Under High Heat Exposure

The ever-increasing complexity in critical spacecraft hardware and materials has led to the development of new microbial reduction procedures as well as to changes in established processes such as heat microbial reduction (HMR). In the space biology field of Planetary Protection, 500°C for 0.5 s is...

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Autores principales: Dean, Zachary Steven, DiNicola, Michael, Klonicki, Emily, Roberts, Scott, Clement, Brian Gregory, Guan, Lisa
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9309537/
https://www.ncbi.nlm.nih.gov/pubmed/35898903
http://dx.doi.org/10.3389/fmicb.2022.909997
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author Dean, Zachary Steven
DiNicola, Michael
Klonicki, Emily
Roberts, Scott
Clement, Brian Gregory
Guan, Lisa
author_facet Dean, Zachary Steven
DiNicola, Michael
Klonicki, Emily
Roberts, Scott
Clement, Brian Gregory
Guan, Lisa
author_sort Dean, Zachary Steven
collection PubMed
description The ever-increasing complexity in critical spacecraft hardware and materials has led to the development of new microbial reduction procedures as well as to changes in established processes such as heat microbial reduction (HMR). In the space biology field of Planetary Protection, 500°C for 0.5 s is the current HMR recommendation to reduce microorganisms from flight hardware. However, more studies are needed to effectively determine the microbial reduction capability of high-temperature (more than 200°C), short-duration (under 30 s) heat exposures. One of the many recent microbial reduction bioengineering research avenues harnesses electromagnetic energy for microbial reduction, with previous investigations demonstrating that infrared heaters are capable of the short temperature ramp time required for rapid heating investigations above 200°C. Therefore, this study employed a 6 kW infrared heater to determine the survivability of heat resistant Bacillus canaveralius 29669 to high-temperature, short-duration infrared temperatures. While B. canaveralius 29669 spores can survive microbial heat reduction processes above 200°C, we found evidence suggesting that the 500°C for 0.5 s temperature sterilization specification for Planetary Protection should be updated. This research presents spore survival data and a corresponding model pointing to a re-evaluation of the recommended HMR exposure of 500°C for 0.5 s, while simultaneously meeting requirements on the forward biological contamination of solar system bodies and opening up design possibilities for future spacecraft hardware.
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spelling pubmed-93095372022-07-26 Establishing Sterility Assurance for Bacillus canaveralius 29669 Spores Under High Heat Exposure Dean, Zachary Steven DiNicola, Michael Klonicki, Emily Roberts, Scott Clement, Brian Gregory Guan, Lisa Front Microbiol Microbiology The ever-increasing complexity in critical spacecraft hardware and materials has led to the development of new microbial reduction procedures as well as to changes in established processes such as heat microbial reduction (HMR). In the space biology field of Planetary Protection, 500°C for 0.5 s is the current HMR recommendation to reduce microorganisms from flight hardware. However, more studies are needed to effectively determine the microbial reduction capability of high-temperature (more than 200°C), short-duration (under 30 s) heat exposures. One of the many recent microbial reduction bioengineering research avenues harnesses electromagnetic energy for microbial reduction, with previous investigations demonstrating that infrared heaters are capable of the short temperature ramp time required for rapid heating investigations above 200°C. Therefore, this study employed a 6 kW infrared heater to determine the survivability of heat resistant Bacillus canaveralius 29669 to high-temperature, short-duration infrared temperatures. While B. canaveralius 29669 spores can survive microbial heat reduction processes above 200°C, we found evidence suggesting that the 500°C for 0.5 s temperature sterilization specification for Planetary Protection should be updated. This research presents spore survival data and a corresponding model pointing to a re-evaluation of the recommended HMR exposure of 500°C for 0.5 s, while simultaneously meeting requirements on the forward biological contamination of solar system bodies and opening up design possibilities for future spacecraft hardware. Frontiers Media S.A. 2022-07-11 /pmc/articles/PMC9309537/ /pubmed/35898903 http://dx.doi.org/10.3389/fmicb.2022.909997 Text en Copyright © 2022 Dean, DiNicola, Klonicki, Roberts, Clement and Guan. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Dean, Zachary Steven
DiNicola, Michael
Klonicki, Emily
Roberts, Scott
Clement, Brian Gregory
Guan, Lisa
Establishing Sterility Assurance for Bacillus canaveralius 29669 Spores Under High Heat Exposure
title Establishing Sterility Assurance for Bacillus canaveralius 29669 Spores Under High Heat Exposure
title_full Establishing Sterility Assurance for Bacillus canaveralius 29669 Spores Under High Heat Exposure
title_fullStr Establishing Sterility Assurance for Bacillus canaveralius 29669 Spores Under High Heat Exposure
title_full_unstemmed Establishing Sterility Assurance for Bacillus canaveralius 29669 Spores Under High Heat Exposure
title_short Establishing Sterility Assurance for Bacillus canaveralius 29669 Spores Under High Heat Exposure
title_sort establishing sterility assurance for bacillus canaveralius 29669 spores under high heat exposure
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9309537/
https://www.ncbi.nlm.nih.gov/pubmed/35898903
http://dx.doi.org/10.3389/fmicb.2022.909997
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