Cargando…

Machine Learning Prediction of Nitrification From Ammonia- and Nitrite-Oxidizer Community Structure

Accurately modeling nitrification and understanding the role specific ammonia- or nitrite-oxidizing taxa play in it are of great interest and importance to microbial ecologists. In this study, we applied machine learning to 16S rRNA sequence and nitrification potential data from an experiment examin...

Descripción completa

Detalles Bibliográficos
Autores principales: Lee, Conard, Amini, Fatemeh, Hu, Guiping, Halverson, Larry J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9309558/
https://www.ncbi.nlm.nih.gov/pubmed/35898910
http://dx.doi.org/10.3389/fmicb.2022.899565
_version_ 1784753192652767232
author Lee, Conard
Amini, Fatemeh
Hu, Guiping
Halverson, Larry J.
author_facet Lee, Conard
Amini, Fatemeh
Hu, Guiping
Halverson, Larry J.
author_sort Lee, Conard
collection PubMed
description Accurately modeling nitrification and understanding the role specific ammonia- or nitrite-oxidizing taxa play in it are of great interest and importance to microbial ecologists. In this study, we applied machine learning to 16S rRNA sequence and nitrification potential data from an experiment examining interactions between cropping systems and rhizosphere on microbial community assembly and nitrogen cycling processes. Given the high dimensionality of microbiome datasets, we only included nitrifers since only a few taxa are capable of ammonia and nitrite oxidation. We compared the performance of linear and nonlinear algorithms with and without qPCR measures of bacterial and archaea ammonia monooxygenase subunit A (amoA) gene abundance. Our feature selection process facilitated the identification of taxons that are most predictive of nitrification and to compare habitats. We found that Nitrosomonas and Nitrospirae were more frequently identified as important predictors of nitrification in conventional systems, whereas Thaumarchaeota were more important predictors in diversified systems. Our results suggest that model performance was not substantively improved by incorporating additional time-consuming and expensive qPCR data on amoA gene abundance. We also identified several clades of nitrifiers important for nitrification in different cropping systems, though we were unable to detect system- or rhizosphere-specific patterns in OTU-level biomarkers for nitrification. Finally, our results highlight the inherent risk of combining data from disparate habitats with the goal of increasing sample size to avoid overfitting models. This study represents a step toward developing machine learning approaches for microbiome research to identify nitrifier ecotypes that may be important for distinguishing ecotypes with defining roles in different habitats.
format Online
Article
Text
id pubmed-9309558
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher Frontiers Media S.A.
record_format MEDLINE/PubMed
spelling pubmed-93095582022-07-26 Machine Learning Prediction of Nitrification From Ammonia- and Nitrite-Oxidizer Community Structure Lee, Conard Amini, Fatemeh Hu, Guiping Halverson, Larry J. Front Microbiol Microbiology Accurately modeling nitrification and understanding the role specific ammonia- or nitrite-oxidizing taxa play in it are of great interest and importance to microbial ecologists. In this study, we applied machine learning to 16S rRNA sequence and nitrification potential data from an experiment examining interactions between cropping systems and rhizosphere on microbial community assembly and nitrogen cycling processes. Given the high dimensionality of microbiome datasets, we only included nitrifers since only a few taxa are capable of ammonia and nitrite oxidation. We compared the performance of linear and nonlinear algorithms with and without qPCR measures of bacterial and archaea ammonia monooxygenase subunit A (amoA) gene abundance. Our feature selection process facilitated the identification of taxons that are most predictive of nitrification and to compare habitats. We found that Nitrosomonas and Nitrospirae were more frequently identified as important predictors of nitrification in conventional systems, whereas Thaumarchaeota were more important predictors in diversified systems. Our results suggest that model performance was not substantively improved by incorporating additional time-consuming and expensive qPCR data on amoA gene abundance. We also identified several clades of nitrifiers important for nitrification in different cropping systems, though we were unable to detect system- or rhizosphere-specific patterns in OTU-level biomarkers for nitrification. Finally, our results highlight the inherent risk of combining data from disparate habitats with the goal of increasing sample size to avoid overfitting models. This study represents a step toward developing machine learning approaches for microbiome research to identify nitrifier ecotypes that may be important for distinguishing ecotypes with defining roles in different habitats. Frontiers Media S.A. 2022-07-11 /pmc/articles/PMC9309558/ /pubmed/35898910 http://dx.doi.org/10.3389/fmicb.2022.899565 Text en Copyright © 2022 Lee, Amini, Hu and Halverson. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Lee, Conard
Amini, Fatemeh
Hu, Guiping
Halverson, Larry J.
Machine Learning Prediction of Nitrification From Ammonia- and Nitrite-Oxidizer Community Structure
title Machine Learning Prediction of Nitrification From Ammonia- and Nitrite-Oxidizer Community Structure
title_full Machine Learning Prediction of Nitrification From Ammonia- and Nitrite-Oxidizer Community Structure
title_fullStr Machine Learning Prediction of Nitrification From Ammonia- and Nitrite-Oxidizer Community Structure
title_full_unstemmed Machine Learning Prediction of Nitrification From Ammonia- and Nitrite-Oxidizer Community Structure
title_short Machine Learning Prediction of Nitrification From Ammonia- and Nitrite-Oxidizer Community Structure
title_sort machine learning prediction of nitrification from ammonia- and nitrite-oxidizer community structure
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9309558/
https://www.ncbi.nlm.nih.gov/pubmed/35898910
http://dx.doi.org/10.3389/fmicb.2022.899565
work_keys_str_mv AT leeconard machinelearningpredictionofnitrificationfromammoniaandnitriteoxidizercommunitystructure
AT aminifatemeh machinelearningpredictionofnitrificationfromammoniaandnitriteoxidizercommunitystructure
AT huguiping machinelearningpredictionofnitrificationfromammoniaandnitriteoxidizercommunitystructure
AT halversonlarryj machinelearningpredictionofnitrificationfromammoniaandnitriteoxidizercommunitystructure