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Solid-state NMR analysis of unlabeled fungal cell walls from Aspergillus and Candida species

Fungal infections cause high mortality in immunocompromised individuals, which has emerged as a significant threat to human health. The efforts devoted to the development of antifungal agents targeting the cell wall polysaccharides have been hindered by our incomplete picture of the assembly and rem...

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Autores principales: Fernando, Liyanage D., Dickwella Widanage, Malitha C., Shekar, S. Chandra, Mentink-Vigier, Frederic, Wang, Ping, Wi, Sungsool, Wang, Tuo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9310124/
https://www.ncbi.nlm.nih.gov/pubmed/35899175
http://dx.doi.org/10.1016/j.yjsbx.2022.100070
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author Fernando, Liyanage D.
Dickwella Widanage, Malitha C.
Shekar, S. Chandra
Mentink-Vigier, Frederic
Wang, Ping
Wi, Sungsool
Wang, Tuo
author_facet Fernando, Liyanage D.
Dickwella Widanage, Malitha C.
Shekar, S. Chandra
Mentink-Vigier, Frederic
Wang, Ping
Wi, Sungsool
Wang, Tuo
author_sort Fernando, Liyanage D.
collection PubMed
description Fungal infections cause high mortality in immunocompromised individuals, which has emerged as a significant threat to human health. The efforts devoted to the development of antifungal agents targeting the cell wall polysaccharides have been hindered by our incomplete picture of the assembly and remodeling of fungal cell walls. High-resolution solid-state nuclear magnetic resonance (ss NMR) studies have substantially revised our understanding of the polymorphic structure of polysaccharides and the nanoscale organization of cell walls in Aspergillus fumigatus and multiple other fungi. However, this approach requires (13)C/(15)N-enrichment of the sample being studied, severely restricting its application. Here we employ the dynamic nuclear polarization (DNP) technique to compare the unlabeled cell wall materials of A. fumigatus and C. albicans prepared using both liquid and solid media. For each fungus, we have identified a highly conserved carbohydrate core for the cell walls of conidia and mycelia, and from liquid and solid cultures. Using samples prepared in different media, the recently identified function of α-glucan, which packs with chitin to form the mechanical centers, has been confirmed through conventional ss NMR measurements of polymer dynamics. These timely efforts not only validate the structural principles recently discovered for A. fumigatus cell walls in different morphological stages, but also open up the possibility of extending the current investigation to other fungal materials and cellular systems that are challenging to label.
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spelling pubmed-93101242022-07-26 Solid-state NMR analysis of unlabeled fungal cell walls from Aspergillus and Candida species Fernando, Liyanage D. Dickwella Widanage, Malitha C. Shekar, S. Chandra Mentink-Vigier, Frederic Wang, Ping Wi, Sungsool Wang, Tuo J Struct Biol X Advances in biomolecular structure and dynamics elucidation by solid-state NMR Fungal infections cause high mortality in immunocompromised individuals, which has emerged as a significant threat to human health. The efforts devoted to the development of antifungal agents targeting the cell wall polysaccharides have been hindered by our incomplete picture of the assembly and remodeling of fungal cell walls. High-resolution solid-state nuclear magnetic resonance (ss NMR) studies have substantially revised our understanding of the polymorphic structure of polysaccharides and the nanoscale organization of cell walls in Aspergillus fumigatus and multiple other fungi. However, this approach requires (13)C/(15)N-enrichment of the sample being studied, severely restricting its application. Here we employ the dynamic nuclear polarization (DNP) technique to compare the unlabeled cell wall materials of A. fumigatus and C. albicans prepared using both liquid and solid media. For each fungus, we have identified a highly conserved carbohydrate core for the cell walls of conidia and mycelia, and from liquid and solid cultures. Using samples prepared in different media, the recently identified function of α-glucan, which packs with chitin to form the mechanical centers, has been confirmed through conventional ss NMR measurements of polymer dynamics. These timely efforts not only validate the structural principles recently discovered for A. fumigatus cell walls in different morphological stages, but also open up the possibility of extending the current investigation to other fungal materials and cellular systems that are challenging to label. Elsevier 2022-07-19 /pmc/articles/PMC9310124/ /pubmed/35899175 http://dx.doi.org/10.1016/j.yjsbx.2022.100070 Text en © 2022 Published by Elsevier Inc. https://creativecommons.org/licenses/by/4.0/This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Advances in biomolecular structure and dynamics elucidation by solid-state NMR
Fernando, Liyanage D.
Dickwella Widanage, Malitha C.
Shekar, S. Chandra
Mentink-Vigier, Frederic
Wang, Ping
Wi, Sungsool
Wang, Tuo
Solid-state NMR analysis of unlabeled fungal cell walls from Aspergillus and Candida species
title Solid-state NMR analysis of unlabeled fungal cell walls from Aspergillus and Candida species
title_full Solid-state NMR analysis of unlabeled fungal cell walls from Aspergillus and Candida species
title_fullStr Solid-state NMR analysis of unlabeled fungal cell walls from Aspergillus and Candida species
title_full_unstemmed Solid-state NMR analysis of unlabeled fungal cell walls from Aspergillus and Candida species
title_short Solid-state NMR analysis of unlabeled fungal cell walls from Aspergillus and Candida species
title_sort solid-state nmr analysis of unlabeled fungal cell walls from aspergillus and candida species
topic Advances in biomolecular structure and dynamics elucidation by solid-state NMR
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9310124/
https://www.ncbi.nlm.nih.gov/pubmed/35899175
http://dx.doi.org/10.1016/j.yjsbx.2022.100070
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