Development of a highly efficient prime editor 2 system in plants

Low efficiency has seriously restricted the application of prime editing (PE) systems in plants. In this study, we develop an enhanced plant prime editor 2 system, enpPE2, by stacking various optimization strategies, including updating the PE architecture to PEmax and expressing engineered pegRNA wi...

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Detalles Bibliográficos
Autores principales: Li, Juan, Chen, Like, Liang, Jing, Xu, Rongfang, Jiang, Yingli, Li, Yizhen, Ding, Jian, Li, Min, Qin, Ruiying, Wei, Pengcheng
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2022
Materias:
Short Report
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9310484/
https://www.ncbi.nlm.nih.gov/pubmed/35879771
http://dx.doi.org/10.1186/s13059-022-02730-x
Descripción
Sumario:Low efficiency has seriously restricted the application of prime editing (PE) systems in plants. In this study, we develop an enhanced plant prime editor 2 system, enpPE2, by stacking various optimization strategies, including updating the PE architecture to PEmax and expressing engineered pegRNA with a structured motif under the control of a composite promoter. In T(0) rice plants, enpPE2 exhibits editing frequencies of 64.58% to 77.08%, which are much higher than the frequencies with unmodified pPE2. Our results indicate that the enpPE2 system provides a robust and powerful tool for the precise modification of plant genomes. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s13059-022-02730-x.

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