Development of a highly efficient prime editor 2 system in plants

Low efficiency has seriously restricted the application of prime editing (PE) systems in plants. In this study, we develop an enhanced plant prime editor 2 system, enpPE2, by stacking various optimization strategies, including updating the PE architecture to PEmax and expressing engineered pegRNA wi...

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Autores principales: Li, Juan, Chen, Like, Liang, Jing, Xu, Rongfang, Jiang, Yingli, Li, Yizhen, Ding, Jian, Li, Min, Qin, Ruiying, Wei, Pengcheng
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2022
Materias:
Short Report
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9310484/
https://www.ncbi.nlm.nih.gov/pubmed/35879771
http://dx.doi.org/10.1186/s13059-022-02730-x
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author Li, Juan
Chen, Like
Liang, Jing
Xu, Rongfang
Jiang, Yingli
Li, Yizhen
Ding, Jian
Li, Min
Qin, Ruiying
Wei, Pengcheng
author_facet Li, Juan
Chen, Like
Liang, Jing
Xu, Rongfang
Jiang, Yingli
Li, Yizhen
Ding, Jian
Li, Min
Qin, Ruiying
Wei, Pengcheng
author_sort Li, Juan
collection PubMed
description Low efficiency has seriously restricted the application of prime editing (PE) systems in plants. In this study, we develop an enhanced plant prime editor 2 system, enpPE2, by stacking various optimization strategies, including updating the PE architecture to PEmax and expressing engineered pegRNA with a structured motif under the control of a composite promoter. In T(0) rice plants, enpPE2 exhibits editing frequencies of 64.58% to 77.08%, which are much higher than the frequencies with unmodified pPE2. Our results indicate that the enpPE2 system provides a robust and powerful tool for the precise modification of plant genomes. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s13059-022-02730-x.
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spelling pubmed-93104842022-07-26 Development of a highly efficient prime editor 2 system in plants Li, Juan Chen, Like Liang, Jing Xu, Rongfang Jiang, Yingli Li, Yizhen Ding, Jian Li, Min Qin, Ruiying Wei, Pengcheng Genome Biol Short Report Low efficiency has seriously restricted the application of prime editing (PE) systems in plants. In this study, we develop an enhanced plant prime editor 2 system, enpPE2, by stacking various optimization strategies, including updating the PE architecture to PEmax and expressing engineered pegRNA with a structured motif under the control of a composite promoter. In T(0) rice plants, enpPE2 exhibits editing frequencies of 64.58% to 77.08%, which are much higher than the frequencies with unmodified pPE2. Our results indicate that the enpPE2 system provides a robust and powerful tool for the precise modification of plant genomes. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s13059-022-02730-x. BioMed Central 2022-07-25 /pmc/articles/PMC9310484/ /pubmed/35879771 http://dx.doi.org/10.1186/s13059-022-02730-x Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
spellingShingle Short Report
Li, Juan
Chen, Like
Liang, Jing
Xu, Rongfang
Jiang, Yingli
Li, Yizhen
Ding, Jian
Li, Min
Qin, Ruiying
Wei, Pengcheng
Development of a highly efficient prime editor 2 system in plants
title Development of a highly efficient prime editor 2 system in plants
title_full Development of a highly efficient prime editor 2 system in plants
title_fullStr Development of a highly efficient prime editor 2 system in plants
title_full_unstemmed Development of a highly efficient prime editor 2 system in plants
title_short Development of a highly efficient prime editor 2 system in plants
title_sort development of a highly efficient prime editor 2 system in plants
topic Short Report
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9310484/
https://www.ncbi.nlm.nih.gov/pubmed/35879771
http://dx.doi.org/10.1186/s13059-022-02730-x
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