Cargando…
Comparison of two staining techniques on the manual and automated canine sperm morphology analysis
Detailed and direct analysis of semen, including sperm morphology, enables a diagnosis of male fertility. This study aimed to describe an economical and verified protocol for canine spermiograms and compare the effectiveness of Sperm Stain(®) and Sperm Blue(®) (Microptic, Spain) in veterinary practi...
Autores principales: | , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
John Wiley and Sons Inc.
2022
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9311200/ https://www.ncbi.nlm.nih.gov/pubmed/35212033 http://dx.doi.org/10.1111/rda.14100 |
_version_ | 1784753555069992960 |
---|---|
author | Surmacz, Paulina Niwinska, Anna Kautz, Ewa Gizinski, Slawomir Faundez, Ricardo |
author_facet | Surmacz, Paulina Niwinska, Anna Kautz, Ewa Gizinski, Slawomir Faundez, Ricardo |
author_sort | Surmacz, Paulina |
collection | PubMed |
description | Detailed and direct analysis of semen, including sperm morphology, enables a diagnosis of male fertility. This study aimed to describe an economical and verified protocol for canine spermiograms and compare the effectiveness of Sperm Stain(®) and Sperm Blue(®) (Microptic, Spain) in veterinary practice. Sperm assessment was conducted manually, using a standard optical microscope, and via computerized semen analysis using the SCA(®) CASA (Sperm Class Analyzer(®) CASA System‐MICROPTIC, Spain). This study showed that Sperm Blue(®) is a better solution for computerized sperm quality analysis of healthy dogs. At the same time, Sperm Stain(®) turned out to be more helpful in identifying specific morphological defects of sperm. Automated canine sperm morphology analysis worked better with Sperm Blue stain, but Sperm Stain simplified manual evaluation of various organelles’ defects. Standard, manual examination is more error‐prone for an inexperienced andrology technician, but it seems to be still a gold standard technique for canine sperm assessment. |
format | Online Article Text |
id | pubmed-9311200 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | John Wiley and Sons Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-93112002022-07-29 Comparison of two staining techniques on the manual and automated canine sperm morphology analysis Surmacz, Paulina Niwinska, Anna Kautz, Ewa Gizinski, Slawomir Faundez, Ricardo Reprod Domest Anim Short Communications Detailed and direct analysis of semen, including sperm morphology, enables a diagnosis of male fertility. This study aimed to describe an economical and verified protocol for canine spermiograms and compare the effectiveness of Sperm Stain(®) and Sperm Blue(®) (Microptic, Spain) in veterinary practice. Sperm assessment was conducted manually, using a standard optical microscope, and via computerized semen analysis using the SCA(®) CASA (Sperm Class Analyzer(®) CASA System‐MICROPTIC, Spain). This study showed that Sperm Blue(®) is a better solution for computerized sperm quality analysis of healthy dogs. At the same time, Sperm Stain(®) turned out to be more helpful in identifying specific morphological defects of sperm. Automated canine sperm morphology analysis worked better with Sperm Blue stain, but Sperm Stain simplified manual evaluation of various organelles’ defects. Standard, manual examination is more error‐prone for an inexperienced andrology technician, but it seems to be still a gold standard technique for canine sperm assessment. John Wiley and Sons Inc. 2022-03-04 2022-06 /pmc/articles/PMC9311200/ /pubmed/35212033 http://dx.doi.org/10.1111/rda.14100 Text en © 2022 The Authors. Reproduction in Domestic Animals published by Wiley‐VCH GmbH. https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc-nd/4.0/ (https://creativecommons.org/licenses/by-nc-nd/4.0/) License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made. |
spellingShingle | Short Communications Surmacz, Paulina Niwinska, Anna Kautz, Ewa Gizinski, Slawomir Faundez, Ricardo Comparison of two staining techniques on the manual and automated canine sperm morphology analysis |
title | Comparison of two staining techniques on the manual and automated canine sperm morphology analysis |
title_full | Comparison of two staining techniques on the manual and automated canine sperm morphology analysis |
title_fullStr | Comparison of two staining techniques on the manual and automated canine sperm morphology analysis |
title_full_unstemmed | Comparison of two staining techniques on the manual and automated canine sperm morphology analysis |
title_short | Comparison of two staining techniques on the manual and automated canine sperm morphology analysis |
title_sort | comparison of two staining techniques on the manual and automated canine sperm morphology analysis |
topic | Short Communications |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9311200/ https://www.ncbi.nlm.nih.gov/pubmed/35212033 http://dx.doi.org/10.1111/rda.14100 |
work_keys_str_mv | AT surmaczpaulina comparisonoftwostainingtechniquesonthemanualandautomatedcaninespermmorphologyanalysis AT niwinskaanna comparisonoftwostainingtechniquesonthemanualandautomatedcaninespermmorphologyanalysis AT kautzewa comparisonoftwostainingtechniquesonthemanualandautomatedcaninespermmorphologyanalysis AT gizinskislawomir comparisonoftwostainingtechniquesonthemanualandautomatedcaninespermmorphologyanalysis AT faundezricardo comparisonoftwostainingtechniquesonthemanualandautomatedcaninespermmorphologyanalysis |