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KANADI promotes thallus differentiation and FR‐induced gametangiophore formation in the liverwort Marchantia
In angiosperms, KANADI transcription factors have roles in the sporophyte generation regulating tissue polarity, organogenesis and shade avoidance responses, but are not required during the gametophyte generation. Whether these roles are conserved in the gametophyte‐dominant bryophyte lineages is un...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
John Wiley and Sons Inc.
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9311212/ https://www.ncbi.nlm.nih.gov/pubmed/35181887 http://dx.doi.org/10.1111/nph.18046 |
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author | Briginshaw, Liam N. Flores‐Sandoval, Eduardo Dierschke, Tom Alvarez, John P. Bowman, John L. |
author_facet | Briginshaw, Liam N. Flores‐Sandoval, Eduardo Dierschke, Tom Alvarez, John P. Bowman, John L. |
author_sort | Briginshaw, Liam N. |
collection | PubMed |
description | In angiosperms, KANADI transcription factors have roles in the sporophyte generation regulating tissue polarity, organogenesis and shade avoidance responses, but are not required during the gametophyte generation. Whether these roles are conserved in the gametophyte‐dominant bryophyte lineages is unknown, which we examined by characterising the sole KANADI ortholog, MpKAN, in the liverwort Marchantia polymorpha. In contrast to angiosperm orthologs, MpKAN functions in the gametophyte generation in Marchantia, where it regulates apical branching and tissue differentiation, but does not influence tissue polarity in either generation. MpKAN can partially rescue the sporophyte polarity defects of kanadi mutants in Arabidopsis, indicating that MpKAN has conserved biochemical activity to its angiosperm counterparts. Mpkan loss‐of‐function plants display defects in far‐red (FR) light responses. Mpkan plants have reduced FR‐induced growth tropisms, have a delayed transition to sexual reproduction and fail to correctly form gametangiophores. Our results indicate that MpKAN is a modulator of FR responses, which may reflect a conserved role for KANADI across land plants. Under FR, MpKAN negatively regulates MpDELLA expression, suggesting that MpKAN and MpDELLA act in a pathway regulating FR responses, placing MpKAN in a gene regulatory network exhibiting similarities with those of angiosperms. |
format | Online Article Text |
id | pubmed-9311212 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | John Wiley and Sons Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-93112122022-07-29 KANADI promotes thallus differentiation and FR‐induced gametangiophore formation in the liverwort Marchantia Briginshaw, Liam N. Flores‐Sandoval, Eduardo Dierschke, Tom Alvarez, John P. Bowman, John L. New Phytol Research In angiosperms, KANADI transcription factors have roles in the sporophyte generation regulating tissue polarity, organogenesis and shade avoidance responses, but are not required during the gametophyte generation. Whether these roles are conserved in the gametophyte‐dominant bryophyte lineages is unknown, which we examined by characterising the sole KANADI ortholog, MpKAN, in the liverwort Marchantia polymorpha. In contrast to angiosperm orthologs, MpKAN functions in the gametophyte generation in Marchantia, where it regulates apical branching and tissue differentiation, but does not influence tissue polarity in either generation. MpKAN can partially rescue the sporophyte polarity defects of kanadi mutants in Arabidopsis, indicating that MpKAN has conserved biochemical activity to its angiosperm counterparts. Mpkan loss‐of‐function plants display defects in far‐red (FR) light responses. Mpkan plants have reduced FR‐induced growth tropisms, have a delayed transition to sexual reproduction and fail to correctly form gametangiophores. Our results indicate that MpKAN is a modulator of FR responses, which may reflect a conserved role for KANADI across land plants. Under FR, MpKAN negatively regulates MpDELLA expression, suggesting that MpKAN and MpDELLA act in a pathway regulating FR responses, placing MpKAN in a gene regulatory network exhibiting similarities with those of angiosperms. John Wiley and Sons Inc. 2022-03-23 2022-05 /pmc/articles/PMC9311212/ /pubmed/35181887 http://dx.doi.org/10.1111/nph.18046 Text en © 2022 The Authors. New Phytologist © 2022 New Phytologist Foundation https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc-nd/4.0/ (https://creativecommons.org/licenses/by-nc-nd/4.0/) License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made. |
spellingShingle | Research Briginshaw, Liam N. Flores‐Sandoval, Eduardo Dierschke, Tom Alvarez, John P. Bowman, John L. KANADI promotes thallus differentiation and FR‐induced gametangiophore formation in the liverwort Marchantia |
title | KANADI promotes thallus differentiation and FR‐induced gametangiophore formation in the liverwort Marchantia
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title_full | KANADI promotes thallus differentiation and FR‐induced gametangiophore formation in the liverwort Marchantia
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title_fullStr | KANADI promotes thallus differentiation and FR‐induced gametangiophore formation in the liverwort Marchantia
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title_full_unstemmed | KANADI promotes thallus differentiation and FR‐induced gametangiophore formation in the liverwort Marchantia
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title_short | KANADI promotes thallus differentiation and FR‐induced gametangiophore formation in the liverwort Marchantia
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title_sort | kanadi promotes thallus differentiation and fr‐induced gametangiophore formation in the liverwort marchantia |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9311212/ https://www.ncbi.nlm.nih.gov/pubmed/35181887 http://dx.doi.org/10.1111/nph.18046 |
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