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A multi‐laboratory assessment of lupus anticoagulant assays performed on the ACL TOP 50 family for harmonized testing in a large laboratory network
INTRODUCTION: Lupus anticoagulant (LA) testing is commonly performed within hemostasis laboratories, and the ACL TOP 50 family of instruments represent a new “single platform” of hemostasis instrumentation. Our aim was to evaluate these instruments and manufacturer reagents or alternatives for utili...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
John Wiley and Sons Inc.
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9311435/ https://www.ncbi.nlm.nih.gov/pubmed/35234361 http://dx.doi.org/10.1111/ijlh.13818 |
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author | Favaloro, Emmanuel J. Mohammed, Soma Vong, Ronny Chapman, Kent Swanepoel, Priscilla Kershaw, Geoffrey Cai, Nancy Just, Sarah Connelly, Lynne Brighton, Timothy Pasalic, Leonardo |
author_facet | Favaloro, Emmanuel J. Mohammed, Soma Vong, Ronny Chapman, Kent Swanepoel, Priscilla Kershaw, Geoffrey Cai, Nancy Just, Sarah Connelly, Lynne Brighton, Timothy Pasalic, Leonardo |
author_sort | Favaloro, Emmanuel J. |
collection | PubMed |
description | INTRODUCTION: Lupus anticoagulant (LA) testing is commonly performed within hemostasis laboratories, and the ACL TOP 50 family of instruments represent a new “single platform” of hemostasis instrumentation. Our aim was to evaluate these instruments and manufacturer reagents or alternatives for utility in LA testing. METHODS: Comparative evaluations of LA testing using newly installed ACL TOPs 550 and 750 as well as comparative assessments with existing “reference,” predominantly Stago, instrumentation, and reagents. Evaluations comprised both dilute Russell viper venom time (dRVVT) and activated partial thromboplastin time (APTT)‐based assays. Establishment of normal reference ranges (NRR). RESULTS: The HemosIL dRVVT‐based assays showed good comparability with the existing Stago reference method (R > 0.9) and could be considered as verified as fit for purpose. A variety of APTT assays was additionally evaluated for LA utility, and we identified from the assessment good utility of a non‐Werfen solution in Hyphen BioMed Cephen reagents. NRR were established based on ≥120 normal individual plasma samples. CONCLUSION: This evaluation of LA reagents on ACL TOP 50 Family instruments identified overall acceptable performance of both dRVVT (Werfen solution) and APTT (non‐Werfen solution) to enable harmonization of LA testing in our large network. |
format | Online Article Text |
id | pubmed-9311435 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | John Wiley and Sons Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-93114352022-07-29 A multi‐laboratory assessment of lupus anticoagulant assays performed on the ACL TOP 50 family for harmonized testing in a large laboratory network Favaloro, Emmanuel J. Mohammed, Soma Vong, Ronny Chapman, Kent Swanepoel, Priscilla Kershaw, Geoffrey Cai, Nancy Just, Sarah Connelly, Lynne Brighton, Timothy Pasalic, Leonardo Int J Lab Hematol Original Articles INTRODUCTION: Lupus anticoagulant (LA) testing is commonly performed within hemostasis laboratories, and the ACL TOP 50 family of instruments represent a new “single platform” of hemostasis instrumentation. Our aim was to evaluate these instruments and manufacturer reagents or alternatives for utility in LA testing. METHODS: Comparative evaluations of LA testing using newly installed ACL TOPs 550 and 750 as well as comparative assessments with existing “reference,” predominantly Stago, instrumentation, and reagents. Evaluations comprised both dilute Russell viper venom time (dRVVT) and activated partial thromboplastin time (APTT)‐based assays. Establishment of normal reference ranges (NRR). RESULTS: The HemosIL dRVVT‐based assays showed good comparability with the existing Stago reference method (R > 0.9) and could be considered as verified as fit for purpose. A variety of APTT assays was additionally evaluated for LA utility, and we identified from the assessment good utility of a non‐Werfen solution in Hyphen BioMed Cephen reagents. NRR were established based on ≥120 normal individual plasma samples. CONCLUSION: This evaluation of LA reagents on ACL TOP 50 Family instruments identified overall acceptable performance of both dRVVT (Werfen solution) and APTT (non‐Werfen solution) to enable harmonization of LA testing in our large network. John Wiley and Sons Inc. 2022-03-01 2022-06 /pmc/articles/PMC9311435/ /pubmed/35234361 http://dx.doi.org/10.1111/ijlh.13818 Text en © 2022 The Authors. International Journal of Laboratory Hematology published by John Wiley & Sons Ltd. https://creativecommons.org/licenses/by/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Original Articles Favaloro, Emmanuel J. Mohammed, Soma Vong, Ronny Chapman, Kent Swanepoel, Priscilla Kershaw, Geoffrey Cai, Nancy Just, Sarah Connelly, Lynne Brighton, Timothy Pasalic, Leonardo A multi‐laboratory assessment of lupus anticoagulant assays performed on the ACL TOP 50 family for harmonized testing in a large laboratory network |
title | A multi‐laboratory assessment of lupus anticoagulant assays performed on the ACL TOP 50 family for harmonized testing in a large laboratory network |
title_full | A multi‐laboratory assessment of lupus anticoagulant assays performed on the ACL TOP 50 family for harmonized testing in a large laboratory network |
title_fullStr | A multi‐laboratory assessment of lupus anticoagulant assays performed on the ACL TOP 50 family for harmonized testing in a large laboratory network |
title_full_unstemmed | A multi‐laboratory assessment of lupus anticoagulant assays performed on the ACL TOP 50 family for harmonized testing in a large laboratory network |
title_short | A multi‐laboratory assessment of lupus anticoagulant assays performed on the ACL TOP 50 family for harmonized testing in a large laboratory network |
title_sort | multi‐laboratory assessment of lupus anticoagulant assays performed on the acl top 50 family for harmonized testing in a large laboratory network |
topic | Original Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9311435/ https://www.ncbi.nlm.nih.gov/pubmed/35234361 http://dx.doi.org/10.1111/ijlh.13818 |
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