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Antioxidant and Neuroprotective Effects of Paeonol against Oxidative Stress and Altered Carrier-Mediated Transport System on NSC-34 Cell Lines

Paeonol is a naturally occurring phenolic agent that attenuates neurotoxicity in neurodegenerative diseases. We aimed to investigate the antioxidant and protective effects of paeonol and determine its transport mechanism in wild-type (WT; NSC-34/hSOD1(WT)) and mutant-type (MT; NSC-34/hSOD1(G93A)) mo...

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Autores principales: Latif, Sana, Choi, Seung-Hye, Gyawali, Asmita, Hyeon, Seung Jae, Kang, Young-Sook, Ryu, Hoon
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9311606/
https://www.ncbi.nlm.nih.gov/pubmed/35883881
http://dx.doi.org/10.3390/antiox11071392
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author Latif, Sana
Choi, Seung-Hye
Gyawali, Asmita
Hyeon, Seung Jae
Kang, Young-Sook
Ryu, Hoon
author_facet Latif, Sana
Choi, Seung-Hye
Gyawali, Asmita
Hyeon, Seung Jae
Kang, Young-Sook
Ryu, Hoon
author_sort Latif, Sana
collection PubMed
description Paeonol is a naturally occurring phenolic agent that attenuates neurotoxicity in neurodegenerative diseases. We aimed to investigate the antioxidant and protective effects of paeonol and determine its transport mechanism in wild-type (WT; NSC-34/hSOD1(WT)) and mutant-type (MT; NSC-34/hSOD1(G93A)) motor neuron-like amyotrophic lateral sclerosis (ALS) cell lines. Cytotoxicity induced by glutamate, lipopolysaccharides, and H(2)O(2) reduced viability of cell; however, the addition of paeonol improved cell viability against neurotoxicity. The [(3)H]paeonol uptake was increased in the presence of H(2)O(2) in both cell lines. Paeonol recovered ALS model cell lines by reducing mitochondrial oxidative stress induced by glutamate. The transport of paeonol was time-, concentration-, and pH-dependent in both NSC-34 cell lines. Kinetic parameters showed two transport sites with altered affinity and capacity in the MT cell line compared to the WT cell line. [(3)H]Paeonol uptake increased in the MT cell line transfected with organic anion transporter1 (Oat1)/Slc22a6 small interfering RNA compared to that in the control. Plasma membrane monoamine transporter (Pmat) was also involved in the uptake of paeonol by ALS model cell lines. Overall, paeonol exhibits neuroprotective activity via a carrier-mediated transport system and may be a beneficial therapy for preventing motor neuronal damage under ALS-like conditions.
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spelling pubmed-93116062022-07-26 Antioxidant and Neuroprotective Effects of Paeonol against Oxidative Stress and Altered Carrier-Mediated Transport System on NSC-34 Cell Lines Latif, Sana Choi, Seung-Hye Gyawali, Asmita Hyeon, Seung Jae Kang, Young-Sook Ryu, Hoon Antioxidants (Basel) Article Paeonol is a naturally occurring phenolic agent that attenuates neurotoxicity in neurodegenerative diseases. We aimed to investigate the antioxidant and protective effects of paeonol and determine its transport mechanism in wild-type (WT; NSC-34/hSOD1(WT)) and mutant-type (MT; NSC-34/hSOD1(G93A)) motor neuron-like amyotrophic lateral sclerosis (ALS) cell lines. Cytotoxicity induced by glutamate, lipopolysaccharides, and H(2)O(2) reduced viability of cell; however, the addition of paeonol improved cell viability against neurotoxicity. The [(3)H]paeonol uptake was increased in the presence of H(2)O(2) in both cell lines. Paeonol recovered ALS model cell lines by reducing mitochondrial oxidative stress induced by glutamate. The transport of paeonol was time-, concentration-, and pH-dependent in both NSC-34 cell lines. Kinetic parameters showed two transport sites with altered affinity and capacity in the MT cell line compared to the WT cell line. [(3)H]Paeonol uptake increased in the MT cell line transfected with organic anion transporter1 (Oat1)/Slc22a6 small interfering RNA compared to that in the control. Plasma membrane monoamine transporter (Pmat) was also involved in the uptake of paeonol by ALS model cell lines. Overall, paeonol exhibits neuroprotective activity via a carrier-mediated transport system and may be a beneficial therapy for preventing motor neuronal damage under ALS-like conditions. MDPI 2022-07-18 /pmc/articles/PMC9311606/ /pubmed/35883881 http://dx.doi.org/10.3390/antiox11071392 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Latif, Sana
Choi, Seung-Hye
Gyawali, Asmita
Hyeon, Seung Jae
Kang, Young-Sook
Ryu, Hoon
Antioxidant and Neuroprotective Effects of Paeonol against Oxidative Stress and Altered Carrier-Mediated Transport System on NSC-34 Cell Lines
title Antioxidant and Neuroprotective Effects of Paeonol against Oxidative Stress and Altered Carrier-Mediated Transport System on NSC-34 Cell Lines
title_full Antioxidant and Neuroprotective Effects of Paeonol against Oxidative Stress and Altered Carrier-Mediated Transport System on NSC-34 Cell Lines
title_fullStr Antioxidant and Neuroprotective Effects of Paeonol against Oxidative Stress and Altered Carrier-Mediated Transport System on NSC-34 Cell Lines
title_full_unstemmed Antioxidant and Neuroprotective Effects of Paeonol against Oxidative Stress and Altered Carrier-Mediated Transport System on NSC-34 Cell Lines
title_short Antioxidant and Neuroprotective Effects of Paeonol against Oxidative Stress and Altered Carrier-Mediated Transport System on NSC-34 Cell Lines
title_sort antioxidant and neuroprotective effects of paeonol against oxidative stress and altered carrier-mediated transport system on nsc-34 cell lines
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9311606/
https://www.ncbi.nlm.nih.gov/pubmed/35883881
http://dx.doi.org/10.3390/antiox11071392
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