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Efficient Robust Yield Method for Preparing Bacterial Ghosts by Escherichia coli Phage ID52 Lysis Protein E

Bacterial ghosts (BGs) are nonliving empty bacterial shells without cytoplasm retaining original morphology and identical antigenicity of natural bacteria, making them high potential and promising vaccine candidates and delivery vehicles. However, the low yield of commonly used BGs preparation metho...

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Autores principales: Ma, Yi, Zhu, Wenjun, Zhu, Guanshu, Xu, Yue, Li, Shuyu, Chen, Rui, Chen, Lidan, Wang, Jufang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9311611/
https://www.ncbi.nlm.nih.gov/pubmed/35877351
http://dx.doi.org/10.3390/bioengineering9070300
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author Ma, Yi
Zhu, Wenjun
Zhu, Guanshu
Xu, Yue
Li, Shuyu
Chen, Rui
Chen, Lidan
Wang, Jufang
author_facet Ma, Yi
Zhu, Wenjun
Zhu, Guanshu
Xu, Yue
Li, Shuyu
Chen, Rui
Chen, Lidan
Wang, Jufang
author_sort Ma, Yi
collection PubMed
description Bacterial ghosts (BGs) are nonliving empty bacterial shells without cytoplasm retaining original morphology and identical antigenicity of natural bacteria, making them high potential and promising vaccine candidates and delivery vehicles. However, the low yield of commonly used BGs preparation methods limits its mass production and widely application. In order to improve BGs production, E. coli phage ID52 lysis protein E was introduced to generating BGs for the first time. Above all, we compared the lysis activity of lysis protein of E. coli phage φX174 and E. coli phage ID52 as well as the effects of promoters on the lysis activity of ID52-E, which shown that the lysis activity and BGs formation rate of protein ID52-E was significantly higher than protein φX174-E. Further, the lysis activity of ID52-E was significantly improved under the control of L-arabinose inducible promoter which initial induction OD(600) reached as high as 2.0. The applicability of lysis protein ID52-E induced by L-arabinose was proved by preparing probiotic E. coli Nissle 1917 BGs and pathogenic Salmonella typhimurium BGs in mass production. This paper introduced a novel and highly efficient method for BGs preparation depending on recombinant expression of E. coli phage ID52-E under eco-friendly and reasonable price inducer L-arabinose.
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spelling pubmed-93116112022-07-26 Efficient Robust Yield Method for Preparing Bacterial Ghosts by Escherichia coli Phage ID52 Lysis Protein E Ma, Yi Zhu, Wenjun Zhu, Guanshu Xu, Yue Li, Shuyu Chen, Rui Chen, Lidan Wang, Jufang Bioengineering (Basel) Article Bacterial ghosts (BGs) are nonliving empty bacterial shells without cytoplasm retaining original morphology and identical antigenicity of natural bacteria, making them high potential and promising vaccine candidates and delivery vehicles. However, the low yield of commonly used BGs preparation methods limits its mass production and widely application. In order to improve BGs production, E. coli phage ID52 lysis protein E was introduced to generating BGs for the first time. Above all, we compared the lysis activity of lysis protein of E. coli phage φX174 and E. coli phage ID52 as well as the effects of promoters on the lysis activity of ID52-E, which shown that the lysis activity and BGs formation rate of protein ID52-E was significantly higher than protein φX174-E. Further, the lysis activity of ID52-E was significantly improved under the control of L-arabinose inducible promoter which initial induction OD(600) reached as high as 2.0. The applicability of lysis protein ID52-E induced by L-arabinose was proved by preparing probiotic E. coli Nissle 1917 BGs and pathogenic Salmonella typhimurium BGs in mass production. This paper introduced a novel and highly efficient method for BGs preparation depending on recombinant expression of E. coli phage ID52-E under eco-friendly and reasonable price inducer L-arabinose. MDPI 2022-07-07 /pmc/articles/PMC9311611/ /pubmed/35877351 http://dx.doi.org/10.3390/bioengineering9070300 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Ma, Yi
Zhu, Wenjun
Zhu, Guanshu
Xu, Yue
Li, Shuyu
Chen, Rui
Chen, Lidan
Wang, Jufang
Efficient Robust Yield Method for Preparing Bacterial Ghosts by Escherichia coli Phage ID52 Lysis Protein E
title Efficient Robust Yield Method for Preparing Bacterial Ghosts by Escherichia coli Phage ID52 Lysis Protein E
title_full Efficient Robust Yield Method for Preparing Bacterial Ghosts by Escherichia coli Phage ID52 Lysis Protein E
title_fullStr Efficient Robust Yield Method for Preparing Bacterial Ghosts by Escherichia coli Phage ID52 Lysis Protein E
title_full_unstemmed Efficient Robust Yield Method for Preparing Bacterial Ghosts by Escherichia coli Phage ID52 Lysis Protein E
title_short Efficient Robust Yield Method for Preparing Bacterial Ghosts by Escherichia coli Phage ID52 Lysis Protein E
title_sort efficient robust yield method for preparing bacterial ghosts by escherichia coli phage id52 lysis protein e
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9311611/
https://www.ncbi.nlm.nih.gov/pubmed/35877351
http://dx.doi.org/10.3390/bioengineering9070300
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