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Subcritical Water as a Pre-Treatment of Mixed Microbial Biomass for the Extraction of Polyhydroxyalkanoates

Polyhydroxyalkanoate (PHA) recovery from microbial cells relies on either solvent extraction (usually using halogenated solvents) and/or digestion of the non-PHA cell mass (NPCM) by the action of chemicals (e.g., hypochlorite) that raise environmental and health hazards. A greener alternative for PH...

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Autores principales: Meneses, Liane, Esmail, Asiyah, Matos, Mariana, Sevrin, Chantal, Grandfils, Christian, Barreiros, Susana, Reis, Maria A. M., Freitas, Filomena, Paiva, Alexandre
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9311994/
https://www.ncbi.nlm.nih.gov/pubmed/35877353
http://dx.doi.org/10.3390/bioengineering9070302
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author Meneses, Liane
Esmail, Asiyah
Matos, Mariana
Sevrin, Chantal
Grandfils, Christian
Barreiros, Susana
Reis, Maria A. M.
Freitas, Filomena
Paiva, Alexandre
author_facet Meneses, Liane
Esmail, Asiyah
Matos, Mariana
Sevrin, Chantal
Grandfils, Christian
Barreiros, Susana
Reis, Maria A. M.
Freitas, Filomena
Paiva, Alexandre
author_sort Meneses, Liane
collection PubMed
description Polyhydroxyalkanoate (PHA) recovery from microbial cells relies on either solvent extraction (usually using halogenated solvents) and/or digestion of the non-PHA cell mass (NPCM) by the action of chemicals (e.g., hypochlorite) that raise environmental and health hazards. A greener alternative for PHA recovery, subcritical water (SBW), was evaluated as a method for the dissolution of the NPCM of a mixed microbial culture (MMC) biomass. A temperature of 150 °C was found as a compromise to reach NPCM solubilization while mostly preventing the degradation of the biopolymer during the procedure. Such conditions yielded a polymer with a purity of 77%. PHA purity was further improved by combining the SBW treatment with hypochlorite digestion, in which a significantly lower hypochlorite concentration (0.1%, v/v) was sufficient to achieve an overall polymer purity of 80%. During the procedure, the biopolymer suffered some depolymerization, as evidenced by the lower molecular weight (M(w)) and higher polydispersity of the extracted samples. Although such changes in the biopolymer’s molecular mass distribution impact its mechanical properties, impairing its utilization in most conventional plastic uses, the obtained PHA can find use in several applications, for example as additives or for the preparation of graft or block co-polymers, in which low-M(w) oligomers are sought.
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spelling pubmed-93119942022-07-26 Subcritical Water as a Pre-Treatment of Mixed Microbial Biomass for the Extraction of Polyhydroxyalkanoates Meneses, Liane Esmail, Asiyah Matos, Mariana Sevrin, Chantal Grandfils, Christian Barreiros, Susana Reis, Maria A. M. Freitas, Filomena Paiva, Alexandre Bioengineering (Basel) Article Polyhydroxyalkanoate (PHA) recovery from microbial cells relies on either solvent extraction (usually using halogenated solvents) and/or digestion of the non-PHA cell mass (NPCM) by the action of chemicals (e.g., hypochlorite) that raise environmental and health hazards. A greener alternative for PHA recovery, subcritical water (SBW), was evaluated as a method for the dissolution of the NPCM of a mixed microbial culture (MMC) biomass. A temperature of 150 °C was found as a compromise to reach NPCM solubilization while mostly preventing the degradation of the biopolymer during the procedure. Such conditions yielded a polymer with a purity of 77%. PHA purity was further improved by combining the SBW treatment with hypochlorite digestion, in which a significantly lower hypochlorite concentration (0.1%, v/v) was sufficient to achieve an overall polymer purity of 80%. During the procedure, the biopolymer suffered some depolymerization, as evidenced by the lower molecular weight (M(w)) and higher polydispersity of the extracted samples. Although such changes in the biopolymer’s molecular mass distribution impact its mechanical properties, impairing its utilization in most conventional plastic uses, the obtained PHA can find use in several applications, for example as additives or for the preparation of graft or block co-polymers, in which low-M(w) oligomers are sought. MDPI 2022-07-08 /pmc/articles/PMC9311994/ /pubmed/35877353 http://dx.doi.org/10.3390/bioengineering9070302 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Meneses, Liane
Esmail, Asiyah
Matos, Mariana
Sevrin, Chantal
Grandfils, Christian
Barreiros, Susana
Reis, Maria A. M.
Freitas, Filomena
Paiva, Alexandre
Subcritical Water as a Pre-Treatment of Mixed Microbial Biomass for the Extraction of Polyhydroxyalkanoates
title Subcritical Water as a Pre-Treatment of Mixed Microbial Biomass for the Extraction of Polyhydroxyalkanoates
title_full Subcritical Water as a Pre-Treatment of Mixed Microbial Biomass for the Extraction of Polyhydroxyalkanoates
title_fullStr Subcritical Water as a Pre-Treatment of Mixed Microbial Biomass for the Extraction of Polyhydroxyalkanoates
title_full_unstemmed Subcritical Water as a Pre-Treatment of Mixed Microbial Biomass for the Extraction of Polyhydroxyalkanoates
title_short Subcritical Water as a Pre-Treatment of Mixed Microbial Biomass for the Extraction of Polyhydroxyalkanoates
title_sort subcritical water as a pre-treatment of mixed microbial biomass for the extraction of polyhydroxyalkanoates
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9311994/
https://www.ncbi.nlm.nih.gov/pubmed/35877353
http://dx.doi.org/10.3390/bioengineering9070302
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