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Puerarin Attenuates Oxidative Stress and Ferroptosis via AMPK/PGC1α/Nrf2 Pathway after Subarachnoid Hemorrhage in Rats

Puerarin was shown to exert anti-oxidative and anti-ferroptosis effects in multiple diseases. The goal of this study was to explore the neuroprotective effect of puerarin on early brain injury (EBI) after subarachnoid hemorrhage (SAH) in rats. A total of 177 adult male Sprague Dawley rats were used....

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Autores principales: Huang, Yi, Wu, Honggang, Hu, Yongmei, Zhou, Chenhui, Wu, Jiawei, Wu, Yiwen, Wang, Haifeng, Lenahan, Cameron, Huang, Lei, Nie, Sheng, Gao, Xiang, Sun, Jie
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9312059/
https://www.ncbi.nlm.nih.gov/pubmed/35883750
http://dx.doi.org/10.3390/antiox11071259
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author Huang, Yi
Wu, Honggang
Hu, Yongmei
Zhou, Chenhui
Wu, Jiawei
Wu, Yiwen
Wang, Haifeng
Lenahan, Cameron
Huang, Lei
Nie, Sheng
Gao, Xiang
Sun, Jie
author_facet Huang, Yi
Wu, Honggang
Hu, Yongmei
Zhou, Chenhui
Wu, Jiawei
Wu, Yiwen
Wang, Haifeng
Lenahan, Cameron
Huang, Lei
Nie, Sheng
Gao, Xiang
Sun, Jie
author_sort Huang, Yi
collection PubMed
description Puerarin was shown to exert anti-oxidative and anti-ferroptosis effects in multiple diseases. The goal of this study was to explore the neuroprotective effect of puerarin on early brain injury (EBI) after subarachnoid hemorrhage (SAH) in rats. A total of 177 adult male Sprague Dawley rats were used. SAH was included via endovascular perforation. Intranasal puerarin or intracerebroventricular dorsomorphin (AMPK inhibitor) and SR18292 (PGC1α inhibitor) were administered. The protein levels of pAMPK, PGC1α, Nrf2, 4HNE, HO1, MDA, ACSL4, GSSG, and iron concentration in the ipsilateral hemisphere were significantly increased, whereas SOD, GPX4, and GSH were decreased at 24 h after SAH. Moreover, puerarin treatment significantly increased the protein levels of pAMPK, PGC1α, Nrf2, HO1, SOD, GPX4, and GSH, but decreased the levels of 4HNE, MDA, ACSL4, GSSG, and iron concentration in the ipsilateral hemisphere at 24 h after SAH. Dorsomorphin or SR18292 partially abolished the beneficial effects of puerarin exerted on neurological dysfunction, oxidative stress injury, and ferroptosis. In conclusion, puerarin improved neurobehavioral impairments and attenuated oxidative-stress-induced brain ferroptosis after SAH in rats. The neuroprotection acted through the activation of the AMPK/PGC1α/Nrf2-signaling pathway. Thus, puerarin may serve as new therapeutics against EBI in SAH patients.
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spelling pubmed-93120592022-07-26 Puerarin Attenuates Oxidative Stress and Ferroptosis via AMPK/PGC1α/Nrf2 Pathway after Subarachnoid Hemorrhage in Rats Huang, Yi Wu, Honggang Hu, Yongmei Zhou, Chenhui Wu, Jiawei Wu, Yiwen Wang, Haifeng Lenahan, Cameron Huang, Lei Nie, Sheng Gao, Xiang Sun, Jie Antioxidants (Basel) Article Puerarin was shown to exert anti-oxidative and anti-ferroptosis effects in multiple diseases. The goal of this study was to explore the neuroprotective effect of puerarin on early brain injury (EBI) after subarachnoid hemorrhage (SAH) in rats. A total of 177 adult male Sprague Dawley rats were used. SAH was included via endovascular perforation. Intranasal puerarin or intracerebroventricular dorsomorphin (AMPK inhibitor) and SR18292 (PGC1α inhibitor) were administered. The protein levels of pAMPK, PGC1α, Nrf2, 4HNE, HO1, MDA, ACSL4, GSSG, and iron concentration in the ipsilateral hemisphere were significantly increased, whereas SOD, GPX4, and GSH were decreased at 24 h after SAH. Moreover, puerarin treatment significantly increased the protein levels of pAMPK, PGC1α, Nrf2, HO1, SOD, GPX4, and GSH, but decreased the levels of 4HNE, MDA, ACSL4, GSSG, and iron concentration in the ipsilateral hemisphere at 24 h after SAH. Dorsomorphin or SR18292 partially abolished the beneficial effects of puerarin exerted on neurological dysfunction, oxidative stress injury, and ferroptosis. In conclusion, puerarin improved neurobehavioral impairments and attenuated oxidative-stress-induced brain ferroptosis after SAH in rats. The neuroprotection acted through the activation of the AMPK/PGC1α/Nrf2-signaling pathway. Thus, puerarin may serve as new therapeutics against EBI in SAH patients. MDPI 2022-06-27 /pmc/articles/PMC9312059/ /pubmed/35883750 http://dx.doi.org/10.3390/antiox11071259 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Huang, Yi
Wu, Honggang
Hu, Yongmei
Zhou, Chenhui
Wu, Jiawei
Wu, Yiwen
Wang, Haifeng
Lenahan, Cameron
Huang, Lei
Nie, Sheng
Gao, Xiang
Sun, Jie
Puerarin Attenuates Oxidative Stress and Ferroptosis via AMPK/PGC1α/Nrf2 Pathway after Subarachnoid Hemorrhage in Rats
title Puerarin Attenuates Oxidative Stress and Ferroptosis via AMPK/PGC1α/Nrf2 Pathway after Subarachnoid Hemorrhage in Rats
title_full Puerarin Attenuates Oxidative Stress and Ferroptosis via AMPK/PGC1α/Nrf2 Pathway after Subarachnoid Hemorrhage in Rats
title_fullStr Puerarin Attenuates Oxidative Stress and Ferroptosis via AMPK/PGC1α/Nrf2 Pathway after Subarachnoid Hemorrhage in Rats
title_full_unstemmed Puerarin Attenuates Oxidative Stress and Ferroptosis via AMPK/PGC1α/Nrf2 Pathway after Subarachnoid Hemorrhage in Rats
title_short Puerarin Attenuates Oxidative Stress and Ferroptosis via AMPK/PGC1α/Nrf2 Pathway after Subarachnoid Hemorrhage in Rats
title_sort puerarin attenuates oxidative stress and ferroptosis via ampk/pgc1α/nrf2 pathway after subarachnoid hemorrhage in rats
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9312059/
https://www.ncbi.nlm.nih.gov/pubmed/35883750
http://dx.doi.org/10.3390/antiox11071259
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