Corynebacterium conjunctivae: A New Corynebacterium Species Isolated from the Ocular Surface of Healthy Horses

SIMPLE SUMMARY: The identification of commensal bacteria in normal eyes is relevant because they contribute to ocular defense mechanisms. Studies focused on identifying the normal ocular flora in horses confirm that Gram-positive bacteria are predominant, with the genus Corynebacterium being among the most frequently identified. However, identification at the species level is uncommon, which limits precise knowledge about the diversity of the corynebacterial species in equine eyes. The purpose of this study was to characterize some commensal Corynebacterium-like organisms recovered from the conjunctival sac of healthy adult horses using phenotypic, chemotaxonomic and molecular genetic methodologies. Based on the results obtained, we propose a new Corynebacterium species, Corynebacterium conjunctivae, as a commensal organism of the horse eye. ABSTRACT: Twenty-two unidentified Gram-positive, rod-shaped organisms were recovered from the conjunctival surface of apparently healthy horses and subjected to a polyphasic taxonomic analysis. Based on cellular morphology and biochemical criteria, the isolates were tentatively assigned to the genus Corynebacterium, although they did not match any recognized species. Comparative 16S rRNA gene sequencing studies demonstrated that all of the isolates were phylogenetically members of the genus Corynebacterium. The isolates shared 99.4 to 100% 16S rRNA gene sequence similarity among the strains and 96.5% similarity with Corynebacterium tapiri 2385/12(T), which was the closest phylogenetically related species. The DNA G+C content was 58.4 mol%. The major fatty acids were C(15:0), C(16:0), C(17:1) ω8c and C(18:1) ω9c, while the predominant mycolic acids consisted of C(30:0), C(32:0) and C(34:0). The isolates were distinguished from related Corynebacterium species by a number of phenotypic properties. On the basis of phenotypic and phylogenetic evidence, it is proposed that the unknown isolates from horses be classified in the genus Corynebacterium as Corynebacterium conjunctivae sp. nov. The type strain of C. conjunctivae is ICM19-01138(T) (DSM 109759(T) = CCUG 73728(T)).