A Diet Supplemented with Polyphenols, Prebiotics and Omega-3 Fatty Acids Modulates the Intestinal Microbiota and Improves the Profile of Metabolites Linked with Anxiety in Dogs

SIMPLE SUMMARY: This study used a nutrition-based approach to examine the effects of foods supplemented with fish oil and a polyphenol blend (citrus pulp, carrot, and spinach) with or without added tomato pomace on anxiety-related biomarkers in dogs. First, all dogs consumed the same initial food, then either the control or test (with tomato pomace) foods, then the washout food, then switched over to the test or control foods, each for 30-day periods. Many more changes in plasma and fecal metabolites were observed when comparing the washout food with the control or test foods than when the control and test foods were compared. Plasma levels of several metabolites that were previously associated with anxiety disorders, including 4-ethylphenyl sulfate, were decreased with the control or test foods compared with the washout food. In addition, bacterial genera that are decreased in the feces of those with anxiety-like disorders were increased following the consumption of the control or test foods. Overall, these data indicate that foods supplemented with omega-3 fatty acids and selected fiber and polyphenol sources lead to beneficial changes in anxiety-related metabolites and gut bacteria. ABSTRACT: A nutrition-based approach was utilized to examine the effects of fish oil and a polyphenol blend (with or without tomato pomace) on the fecal microbiota and plasma/fecal metabolomes. Forty dogs, aged 5–14 years, were fed a washout food, then randomized to consume a control (fish oil and polyphenol blend without tomato pomace) or test (fish oil and polyphenol blend with tomato pomace) food, then the washout food, and crossed over to consume the test or control food; each for 30 days. Several metabolites differed when comparing consumption of the washout with either the control or test foods, but few changed significantly between the test and control foods. Plasma levels of 4-ethylphenyl sulfate (4-EPS), a metabolite associated with anxiety disorders, demonstrated the largest decrease between the washout food and the control/test foods. Plasma 4-EPS levels were also significantly lower after dogs ate the test food compared with the control food. Other plasma metabolites linked with anxiety disorders were decreased following consumption of the control/test foods. Significant increases in Blautia, Parabacteroides, and Odoribacter in the fecal microbiota correlated with decreases in 4-EPS when dogs ate the control/test foods. These data indicate that foods supplemented with polyphenols and omega-3 fatty acids can modulate the gut microbiota to improve the profile of anxiety-linked metabolites.