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Transcriptomic and Metabolomic Analysis of the Response of Quinoa Seedlings to Low Temperatures

Quinoa, a cool-weather high-altitude crop, is susceptible to low-temperature stress throughout its reproductive phase. Herein, we performed broadly targeted metabolic profiling of quinoa seedlings to explore the metabolites’ dynamics in response to low-temperature stress and transcriptome analysis t...

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Autores principales: Xie, Heng, Wang, Qianchao, Zhang, Ping, Zhang, Xuesong, Huang, Tingzhi, Guo, Yirui, Liu, Junna, Li, Li, Li, Hanxue, Qin, Peng
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9312504/
https://www.ncbi.nlm.nih.gov/pubmed/35883533
http://dx.doi.org/10.3390/biom12070977
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author Xie, Heng
Wang, Qianchao
Zhang, Ping
Zhang, Xuesong
Huang, Tingzhi
Guo, Yirui
Liu, Junna
Li, Li
Li, Hanxue
Qin, Peng
author_facet Xie, Heng
Wang, Qianchao
Zhang, Ping
Zhang, Xuesong
Huang, Tingzhi
Guo, Yirui
Liu, Junna
Li, Li
Li, Hanxue
Qin, Peng
author_sort Xie, Heng
collection PubMed
description Quinoa, a cool-weather high-altitude crop, is susceptible to low-temperature stress throughout its reproductive phase. Herein, we performed broadly targeted metabolic profiling of quinoa seedlings to explore the metabolites’ dynamics in response to low-temperature stress and transcriptome analysis to determine the underlying genetic mechanisms. Two variants, namely, Dian Quinoa 2324 and Dian Quinoa 281, were exposed to temperatures of −2, 5, and 22 °C. A total of 794 metabolites were detected; 52,845 genes, including 6628 novel genes, were annotated using UPLC-MS/MS analysis and the Illumina HiSeq system. Combined with morphological indicators to resolve the mechanism underlying quinoa seedling response to low-temperature stress, the molecular mechanisms of quinoa changed considerably based on temperature exposure. Soluble sugars heavily accumulated in plants with cold damage and changes in regulatory networks under freeze damage, such as the upregulation of α-linolenic acid metabolism and a reduction in energy substrates, may explain the spatial patterns of biosynthesis and accumulation of these metabolites. Genes that are actively expressed during cold responses, as revealed by co-expression analyses, may be involved in the regulation thereof. These results provide insights into the metabolic factors in quinoa under low-temperature stress and provide a reference for the screening of quinoa varieties resistant to low temperature.
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spelling pubmed-93125042022-07-26 Transcriptomic and Metabolomic Analysis of the Response of Quinoa Seedlings to Low Temperatures Xie, Heng Wang, Qianchao Zhang, Ping Zhang, Xuesong Huang, Tingzhi Guo, Yirui Liu, Junna Li, Li Li, Hanxue Qin, Peng Biomolecules Article Quinoa, a cool-weather high-altitude crop, is susceptible to low-temperature stress throughout its reproductive phase. Herein, we performed broadly targeted metabolic profiling of quinoa seedlings to explore the metabolites’ dynamics in response to low-temperature stress and transcriptome analysis to determine the underlying genetic mechanisms. Two variants, namely, Dian Quinoa 2324 and Dian Quinoa 281, were exposed to temperatures of −2, 5, and 22 °C. A total of 794 metabolites were detected; 52,845 genes, including 6628 novel genes, were annotated using UPLC-MS/MS analysis and the Illumina HiSeq system. Combined with morphological indicators to resolve the mechanism underlying quinoa seedling response to low-temperature stress, the molecular mechanisms of quinoa changed considerably based on temperature exposure. Soluble sugars heavily accumulated in plants with cold damage and changes in regulatory networks under freeze damage, such as the upregulation of α-linolenic acid metabolism and a reduction in energy substrates, may explain the spatial patterns of biosynthesis and accumulation of these metabolites. Genes that are actively expressed during cold responses, as revealed by co-expression analyses, may be involved in the regulation thereof. These results provide insights into the metabolic factors in quinoa under low-temperature stress and provide a reference for the screening of quinoa varieties resistant to low temperature. MDPI 2022-07-12 /pmc/articles/PMC9312504/ /pubmed/35883533 http://dx.doi.org/10.3390/biom12070977 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Xie, Heng
Wang, Qianchao
Zhang, Ping
Zhang, Xuesong
Huang, Tingzhi
Guo, Yirui
Liu, Junna
Li, Li
Li, Hanxue
Qin, Peng
Transcriptomic and Metabolomic Analysis of the Response of Quinoa Seedlings to Low Temperatures
title Transcriptomic and Metabolomic Analysis of the Response of Quinoa Seedlings to Low Temperatures
title_full Transcriptomic and Metabolomic Analysis of the Response of Quinoa Seedlings to Low Temperatures
title_fullStr Transcriptomic and Metabolomic Analysis of the Response of Quinoa Seedlings to Low Temperatures
title_full_unstemmed Transcriptomic and Metabolomic Analysis of the Response of Quinoa Seedlings to Low Temperatures
title_short Transcriptomic and Metabolomic Analysis of the Response of Quinoa Seedlings to Low Temperatures
title_sort transcriptomic and metabolomic analysis of the response of quinoa seedlings to low temperatures
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9312504/
https://www.ncbi.nlm.nih.gov/pubmed/35883533
http://dx.doi.org/10.3390/biom12070977
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