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Comparison of Two Nuclear Magnetic Resonance Spectroscopy Methods for the Measurement of Lipoprotein Particle Concentrations

Background: Measuring lipoprotein particle concentrations may help to improve cardiovascular risk stratification. Both the lipofit (Numares) and lipoprofile (LabCorp) NMR methods are widely used for the quantification of lipoprotein particle concentrations. Objective: The aim of the present work was...

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Autores principales: Rief, Martin, Raggam, Reinhard, Rief, Peter, Metnitz, Philipp, Stojakovic, Tatjana, Reinthaler, Markus, Brodmann, Marianne, März, Winfried, Scharnagl, Hubert, Silbernagel, Günther
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9312544/
https://www.ncbi.nlm.nih.gov/pubmed/35885071
http://dx.doi.org/10.3390/biomedicines10071766
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author Rief, Martin
Raggam, Reinhard
Rief, Peter
Metnitz, Philipp
Stojakovic, Tatjana
Reinthaler, Markus
Brodmann, Marianne
März, Winfried
Scharnagl, Hubert
Silbernagel, Günther
author_facet Rief, Martin
Raggam, Reinhard
Rief, Peter
Metnitz, Philipp
Stojakovic, Tatjana
Reinthaler, Markus
Brodmann, Marianne
März, Winfried
Scharnagl, Hubert
Silbernagel, Günther
author_sort Rief, Martin
collection PubMed
description Background: Measuring lipoprotein particle concentrations may help to improve cardiovascular risk stratification. Both the lipofit (Numares) and lipoprofile (LabCorp) NMR methods are widely used for the quantification of lipoprotein particle concentrations. Objective: The aim of the present work was to perform a method comparison between the lipofit and lipoprofile NMR methods. In addition, there was the objective to compare lipofit and lipoprofile measurements of standard lipids with clinical chemistry-based results. Methods: Total, LDL, and HDL cholesterol and triglycerides were measured with ß-quantification in serum samples from 150 individuals. NMR measurements of standard lipids and lipoprotein particle concentrations were performed by Numares and LabCorp. Results: For both NMR methods, differences of mean concentrations compared to ß-quantification-derived measurements were ≤5.5% for all standard lipids. There was a strong correlation between ß-quantification- and NMR-derived measurements of total and LDL cholesterol and triglycerides (all r > 0.93). For both, the lipofit (r = 0.81) and lipoprofile (r = 0.84) methods, correlation coefficients were lower for HDL cholesterol. There was a reasonable correlation between LDL and HDL lipoprotein particle concentrations measured with both NMR methods (both r > 0.9). However, mean concentrations of major and subclass lipoprotein particle concentrations were not as strong. Conclusions: The present analysis suggests that reliable measurement of standard lipids is possible with these two NMR methods. Harmonization efforts would be needed for better comparability of particle concentration data.
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spelling pubmed-93125442022-07-26 Comparison of Two Nuclear Magnetic Resonance Spectroscopy Methods for the Measurement of Lipoprotein Particle Concentrations Rief, Martin Raggam, Reinhard Rief, Peter Metnitz, Philipp Stojakovic, Tatjana Reinthaler, Markus Brodmann, Marianne März, Winfried Scharnagl, Hubert Silbernagel, Günther Biomedicines Article Background: Measuring lipoprotein particle concentrations may help to improve cardiovascular risk stratification. Both the lipofit (Numares) and lipoprofile (LabCorp) NMR methods are widely used for the quantification of lipoprotein particle concentrations. Objective: The aim of the present work was to perform a method comparison between the lipofit and lipoprofile NMR methods. In addition, there was the objective to compare lipofit and lipoprofile measurements of standard lipids with clinical chemistry-based results. Methods: Total, LDL, and HDL cholesterol and triglycerides were measured with ß-quantification in serum samples from 150 individuals. NMR measurements of standard lipids and lipoprotein particle concentrations were performed by Numares and LabCorp. Results: For both NMR methods, differences of mean concentrations compared to ß-quantification-derived measurements were ≤5.5% for all standard lipids. There was a strong correlation between ß-quantification- and NMR-derived measurements of total and LDL cholesterol and triglycerides (all r > 0.93). For both, the lipofit (r = 0.81) and lipoprofile (r = 0.84) methods, correlation coefficients were lower for HDL cholesterol. There was a reasonable correlation between LDL and HDL lipoprotein particle concentrations measured with both NMR methods (both r > 0.9). However, mean concentrations of major and subclass lipoprotein particle concentrations were not as strong. Conclusions: The present analysis suggests that reliable measurement of standard lipids is possible with these two NMR methods. Harmonization efforts would be needed for better comparability of particle concentration data. MDPI 2022-07-21 /pmc/articles/PMC9312544/ /pubmed/35885071 http://dx.doi.org/10.3390/biomedicines10071766 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Rief, Martin
Raggam, Reinhard
Rief, Peter
Metnitz, Philipp
Stojakovic, Tatjana
Reinthaler, Markus
Brodmann, Marianne
März, Winfried
Scharnagl, Hubert
Silbernagel, Günther
Comparison of Two Nuclear Magnetic Resonance Spectroscopy Methods for the Measurement of Lipoprotein Particle Concentrations
title Comparison of Two Nuclear Magnetic Resonance Spectroscopy Methods for the Measurement of Lipoprotein Particle Concentrations
title_full Comparison of Two Nuclear Magnetic Resonance Spectroscopy Methods for the Measurement of Lipoprotein Particle Concentrations
title_fullStr Comparison of Two Nuclear Magnetic Resonance Spectroscopy Methods for the Measurement of Lipoprotein Particle Concentrations
title_full_unstemmed Comparison of Two Nuclear Magnetic Resonance Spectroscopy Methods for the Measurement of Lipoprotein Particle Concentrations
title_short Comparison of Two Nuclear Magnetic Resonance Spectroscopy Methods for the Measurement of Lipoprotein Particle Concentrations
title_sort comparison of two nuclear magnetic resonance spectroscopy methods for the measurement of lipoprotein particle concentrations
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9312544/
https://www.ncbi.nlm.nih.gov/pubmed/35885071
http://dx.doi.org/10.3390/biomedicines10071766
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