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Inhibition of Agrobacterium tumefaciens Growth and Biofilm Formation by Tannic Acid

Agrobacterium tumefaciens underlies the pathogenesis of crown gall disease and is characterized by tumor-like gall formation on the stems and roots of a wide variety of economically important plant species. The bacterium initiates infection by colonizing and forming biofilms on plant surfaces, and t...

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Autores principales: Jailani, Afreen, Ahmed, Bilal, Lee, Jin-Hyung, Lee, Jintae
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9312696/
https://www.ncbi.nlm.nih.gov/pubmed/35884920
http://dx.doi.org/10.3390/biomedicines10071619
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author Jailani, Afreen
Ahmed, Bilal
Lee, Jin-Hyung
Lee, Jintae
author_facet Jailani, Afreen
Ahmed, Bilal
Lee, Jin-Hyung
Lee, Jintae
author_sort Jailani, Afreen
collection PubMed
description Agrobacterium tumefaciens underlies the pathogenesis of crown gall disease and is characterized by tumor-like gall formation on the stems and roots of a wide variety of economically important plant species. The bacterium initiates infection by colonizing and forming biofilms on plant surfaces, and thus, novel compounds are required to prevent its growth and biofilm formation. In this study, we investigated the ability of tannic acid, which is ubiquitously present in woody plants, to specifically inhibit the growth and biofilm formation of A. tumefaciens. Tannic acid showed antibacterial activity and significantly reduced the biofilm formation on polystyrene and on the roots of Raphanus sativus as determined by 3D bright-field and scanning electron microscopy (SEM) images. Furthermore, tannic acid dose-dependently reduced the virulence features of A. tumefaciens, which are swimming motility, exopolysaccharide production, protease production, and cell surface hydrophobicity. Transcriptional analysis of cells (Abs600 nm = 1.0) incubated with tannic acid for 24 h at 30 °C showed tannic acid most significantly downregulated the exoR gene, which is required for adhesion to surfaces. Tannic acid at 100 or 200 µg/mL limited the iron supply to A. tumefaciens and similarly reduced the biofilm formation to that performed by 0.1 mM EDTA. Notably, tannic acid did not significantly affect R. sativus germination even at 400 µg/mL. The findings of this study suggest that tannic acid has the potential to prevent growth and biofilm formation by A. tumefaciens and thus infections resulting from A. tumefaciens colonization.
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spelling pubmed-93126962022-07-26 Inhibition of Agrobacterium tumefaciens Growth and Biofilm Formation by Tannic Acid Jailani, Afreen Ahmed, Bilal Lee, Jin-Hyung Lee, Jintae Biomedicines Article Agrobacterium tumefaciens underlies the pathogenesis of crown gall disease and is characterized by tumor-like gall formation on the stems and roots of a wide variety of economically important plant species. The bacterium initiates infection by colonizing and forming biofilms on plant surfaces, and thus, novel compounds are required to prevent its growth and biofilm formation. In this study, we investigated the ability of tannic acid, which is ubiquitously present in woody plants, to specifically inhibit the growth and biofilm formation of A. tumefaciens. Tannic acid showed antibacterial activity and significantly reduced the biofilm formation on polystyrene and on the roots of Raphanus sativus as determined by 3D bright-field and scanning electron microscopy (SEM) images. Furthermore, tannic acid dose-dependently reduced the virulence features of A. tumefaciens, which are swimming motility, exopolysaccharide production, protease production, and cell surface hydrophobicity. Transcriptional analysis of cells (Abs600 nm = 1.0) incubated with tannic acid for 24 h at 30 °C showed tannic acid most significantly downregulated the exoR gene, which is required for adhesion to surfaces. Tannic acid at 100 or 200 µg/mL limited the iron supply to A. tumefaciens and similarly reduced the biofilm formation to that performed by 0.1 mM EDTA. Notably, tannic acid did not significantly affect R. sativus germination even at 400 µg/mL. The findings of this study suggest that tannic acid has the potential to prevent growth and biofilm formation by A. tumefaciens and thus infections resulting from A. tumefaciens colonization. MDPI 2022-07-06 /pmc/articles/PMC9312696/ /pubmed/35884920 http://dx.doi.org/10.3390/biomedicines10071619 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Jailani, Afreen
Ahmed, Bilal
Lee, Jin-Hyung
Lee, Jintae
Inhibition of Agrobacterium tumefaciens Growth and Biofilm Formation by Tannic Acid
title Inhibition of Agrobacterium tumefaciens Growth and Biofilm Formation by Tannic Acid
title_full Inhibition of Agrobacterium tumefaciens Growth and Biofilm Formation by Tannic Acid
title_fullStr Inhibition of Agrobacterium tumefaciens Growth and Biofilm Formation by Tannic Acid
title_full_unstemmed Inhibition of Agrobacterium tumefaciens Growth and Biofilm Formation by Tannic Acid
title_short Inhibition of Agrobacterium tumefaciens Growth and Biofilm Formation by Tannic Acid
title_sort inhibition of agrobacterium tumefaciens growth and biofilm formation by tannic acid
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9312696/
https://www.ncbi.nlm.nih.gov/pubmed/35884920
http://dx.doi.org/10.3390/biomedicines10071619
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