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Differential Refractometric Biosensor for Reliable Human IgG Detection: Proof of Concept

A new sensing platform based on long-period fiber gratings (LPFGs) for direct, fast, and selective detection of human immunoglobulin G (IgG; Mw = 150 KDa) was developed and characterized. The transducer’s high selectivity is based on the specific interaction of a molecularly imprinted polymer (MIPs)...

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Autores principales: Mendes, João P., Coelho, Luís C. C., Jorge, Pedro A. S., Pereira, Carlos M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9312733/
https://www.ncbi.nlm.nih.gov/pubmed/35884318
http://dx.doi.org/10.3390/bios12070515
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author Mendes, João P.
Coelho, Luís C. C.
Jorge, Pedro A. S.
Pereira, Carlos M.
author_facet Mendes, João P.
Coelho, Luís C. C.
Jorge, Pedro A. S.
Pereira, Carlos M.
author_sort Mendes, João P.
collection PubMed
description A new sensing platform based on long-period fiber gratings (LPFGs) for direct, fast, and selective detection of human immunoglobulin G (IgG; Mw = 150 KDa) was developed and characterized. The transducer’s high selectivity is based on the specific interaction of a molecularly imprinted polymer (MIPs) design for IgG detection. The sensing scheme is based on differential refractometric measurements, including a correction system based on a non-imprinted polymer (NIP)-coated LPFG, allowing reliable and more sensitive measurements, improving the rejection of false positives in around 30%. The molecular imprinted binding sites were performed on the surface of a LPFG with a sensitivity of about 130 nm/RIU and a FOM of 16 RIU(−1). The low-cost and easy to build device was tested in a working range from 1 to 100 nmol/L, revealing a limit of detection (LOD) and a sensitivity of 0.25 nmol/L (0.037 µg/mL) and 0.057 nm.L/nmol, respectively. The sensor also successfully differentiates the target analyte from the other abundant elements that are present in the human blood plasma.
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spelling pubmed-93127332022-07-26 Differential Refractometric Biosensor for Reliable Human IgG Detection: Proof of Concept Mendes, João P. Coelho, Luís C. C. Jorge, Pedro A. S. Pereira, Carlos M. Biosensors (Basel) Article A new sensing platform based on long-period fiber gratings (LPFGs) for direct, fast, and selective detection of human immunoglobulin G (IgG; Mw = 150 KDa) was developed and characterized. The transducer’s high selectivity is based on the specific interaction of a molecularly imprinted polymer (MIPs) design for IgG detection. The sensing scheme is based on differential refractometric measurements, including a correction system based on a non-imprinted polymer (NIP)-coated LPFG, allowing reliable and more sensitive measurements, improving the rejection of false positives in around 30%. The molecular imprinted binding sites were performed on the surface of a LPFG with a sensitivity of about 130 nm/RIU and a FOM of 16 RIU(−1). The low-cost and easy to build device was tested in a working range from 1 to 100 nmol/L, revealing a limit of detection (LOD) and a sensitivity of 0.25 nmol/L (0.037 µg/mL) and 0.057 nm.L/nmol, respectively. The sensor also successfully differentiates the target analyte from the other abundant elements that are present in the human blood plasma. MDPI 2022-07-12 /pmc/articles/PMC9312733/ /pubmed/35884318 http://dx.doi.org/10.3390/bios12070515 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Mendes, João P.
Coelho, Luís C. C.
Jorge, Pedro A. S.
Pereira, Carlos M.
Differential Refractometric Biosensor for Reliable Human IgG Detection: Proof of Concept
title Differential Refractometric Biosensor for Reliable Human IgG Detection: Proof of Concept
title_full Differential Refractometric Biosensor for Reliable Human IgG Detection: Proof of Concept
title_fullStr Differential Refractometric Biosensor for Reliable Human IgG Detection: Proof of Concept
title_full_unstemmed Differential Refractometric Biosensor for Reliable Human IgG Detection: Proof of Concept
title_short Differential Refractometric Biosensor for Reliable Human IgG Detection: Proof of Concept
title_sort differential refractometric biosensor for reliable human igg detection: proof of concept
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9312733/
https://www.ncbi.nlm.nih.gov/pubmed/35884318
http://dx.doi.org/10.3390/bios12070515
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