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3D Human Organoids: The Next “Viral” Model for the Molecular Basis of Infectious Diseases

The COVID-19 pandemic has driven the scientific community to adopt an efficient and reliable model that could keep up with the infectious disease arms race. Coinciding with the pandemic, three dimensional (3D) human organoids technology has also gained traction in the field of infectious disease. An...

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Autores principales: Chia, Shirley Pei Shan, Kong, Sharleen Li Ying, Pang, Jeremy Kah Sheng, Soh, Boon-Seng
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9312734/
https://www.ncbi.nlm.nih.gov/pubmed/35884846
http://dx.doi.org/10.3390/biomedicines10071541
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author Chia, Shirley Pei Shan
Kong, Sharleen Li Ying
Pang, Jeremy Kah Sheng
Soh, Boon-Seng
author_facet Chia, Shirley Pei Shan
Kong, Sharleen Li Ying
Pang, Jeremy Kah Sheng
Soh, Boon-Seng
author_sort Chia, Shirley Pei Shan
collection PubMed
description The COVID-19 pandemic has driven the scientific community to adopt an efficient and reliable model that could keep up with the infectious disease arms race. Coinciding with the pandemic, three dimensional (3D) human organoids technology has also gained traction in the field of infectious disease. An in vitro construct that can closely resemble the in vivo organ, organoid technology could bridge the gap between the traditional two-dimensional (2D) cell culture and animal models. By harnessing the multi-lineage characteristic of the organoid that allows for the recapitulation of the organotypic structure and functions, 3D human organoids have emerged as an essential tool in the field of infectious disease research. In this review, we will be providing a comparison between conventional systems and organoid models. We will also be highlighting how organoids played a role in modelling common infectious diseases and molecular mechanisms behind the pathogenesis of causative agents. Additionally, we present the limitations associated with the current organoid models and innovative strategies that could resolve these shortcomings.
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spelling pubmed-93127342022-07-26 3D Human Organoids: The Next “Viral” Model for the Molecular Basis of Infectious Diseases Chia, Shirley Pei Shan Kong, Sharleen Li Ying Pang, Jeremy Kah Sheng Soh, Boon-Seng Biomedicines Review The COVID-19 pandemic has driven the scientific community to adopt an efficient and reliable model that could keep up with the infectious disease arms race. Coinciding with the pandemic, three dimensional (3D) human organoids technology has also gained traction in the field of infectious disease. An in vitro construct that can closely resemble the in vivo organ, organoid technology could bridge the gap between the traditional two-dimensional (2D) cell culture and animal models. By harnessing the multi-lineage characteristic of the organoid that allows for the recapitulation of the organotypic structure and functions, 3D human organoids have emerged as an essential tool in the field of infectious disease research. In this review, we will be providing a comparison between conventional systems and organoid models. We will also be highlighting how organoids played a role in modelling common infectious diseases and molecular mechanisms behind the pathogenesis of causative agents. Additionally, we present the limitations associated with the current organoid models and innovative strategies that could resolve these shortcomings. MDPI 2022-06-28 /pmc/articles/PMC9312734/ /pubmed/35884846 http://dx.doi.org/10.3390/biomedicines10071541 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Review
Chia, Shirley Pei Shan
Kong, Sharleen Li Ying
Pang, Jeremy Kah Sheng
Soh, Boon-Seng
3D Human Organoids: The Next “Viral” Model for the Molecular Basis of Infectious Diseases
title 3D Human Organoids: The Next “Viral” Model for the Molecular Basis of Infectious Diseases
title_full 3D Human Organoids: The Next “Viral” Model for the Molecular Basis of Infectious Diseases
title_fullStr 3D Human Organoids: The Next “Viral” Model for the Molecular Basis of Infectious Diseases
title_full_unstemmed 3D Human Organoids: The Next “Viral” Model for the Molecular Basis of Infectious Diseases
title_short 3D Human Organoids: The Next “Viral” Model for the Molecular Basis of Infectious Diseases
title_sort 3d human organoids: the next “viral” model for the molecular basis of infectious diseases
topic Review
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9312734/
https://www.ncbi.nlm.nih.gov/pubmed/35884846
http://dx.doi.org/10.3390/biomedicines10071541
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