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Multifrequency Investigation of Single- and Double-Stranded DNA with Scalable Metamaterial-Based THz Biosensors

Due to the occurrence of THz-excited vibrational modes in biomacromolecules, the THz frequency range has been identified as particularly suitable for developing and applying new bioanalytical methods. We present a scalable THz metamaterial-based biosensor being utilized for the multifrequency invest...

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Autores principales: Weisenstein, Christian, Richter, Merle, Wigger, Anna Katharina, Bosserhoff, Anja K., Haring Bolívar, Peter
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9312848/
https://www.ncbi.nlm.nih.gov/pubmed/35884286
http://dx.doi.org/10.3390/bios12070483
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author Weisenstein, Christian
Richter, Merle
Wigger, Anna Katharina
Bosserhoff, Anja K.
Haring Bolívar, Peter
author_facet Weisenstein, Christian
Richter, Merle
Wigger, Anna Katharina
Bosserhoff, Anja K.
Haring Bolívar, Peter
author_sort Weisenstein, Christian
collection PubMed
description Due to the occurrence of THz-excited vibrational modes in biomacromolecules, the THz frequency range has been identified as particularly suitable for developing and applying new bioanalytical methods. We present a scalable THz metamaterial-based biosensor being utilized for the multifrequency investigation of single- and double-stranded DNA (ssDNA and dsDNA) samples. It is demonstrated that the metamaterial resonance frequency shift by the DNA’s presence depends on frequency. Our experiments with the scalable THz biosensors demonstrate a major change in the degree of the power function for dsDNA by 1.53 ± 0.06 and, in comparison, 0.34 ± 0.11 for ssDNA as a function of metamaterial resonance frequency. Thus, there is a significant advantage for dsDNA detection that can be used for increased sensitivity of biomolecular detection at higher frequencies. This work represents a first step for application-specific biosensors with potential advantages in sensitivity, specificity, and robustness.
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spelling pubmed-93128482022-07-26 Multifrequency Investigation of Single- and Double-Stranded DNA with Scalable Metamaterial-Based THz Biosensors Weisenstein, Christian Richter, Merle Wigger, Anna Katharina Bosserhoff, Anja K. Haring Bolívar, Peter Biosensors (Basel) Article Due to the occurrence of THz-excited vibrational modes in biomacromolecules, the THz frequency range has been identified as particularly suitable for developing and applying new bioanalytical methods. We present a scalable THz metamaterial-based biosensor being utilized for the multifrequency investigation of single- and double-stranded DNA (ssDNA and dsDNA) samples. It is demonstrated that the metamaterial resonance frequency shift by the DNA’s presence depends on frequency. Our experiments with the scalable THz biosensors demonstrate a major change in the degree of the power function for dsDNA by 1.53 ± 0.06 and, in comparison, 0.34 ± 0.11 for ssDNA as a function of metamaterial resonance frequency. Thus, there is a significant advantage for dsDNA detection that can be used for increased sensitivity of biomolecular detection at higher frequencies. This work represents a first step for application-specific biosensors with potential advantages in sensitivity, specificity, and robustness. MDPI 2022-07-01 /pmc/articles/PMC9312848/ /pubmed/35884286 http://dx.doi.org/10.3390/bios12070483 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Weisenstein, Christian
Richter, Merle
Wigger, Anna Katharina
Bosserhoff, Anja K.
Haring Bolívar, Peter
Multifrequency Investigation of Single- and Double-Stranded DNA with Scalable Metamaterial-Based THz Biosensors
title Multifrequency Investigation of Single- and Double-Stranded DNA with Scalable Metamaterial-Based THz Biosensors
title_full Multifrequency Investigation of Single- and Double-Stranded DNA with Scalable Metamaterial-Based THz Biosensors
title_fullStr Multifrequency Investigation of Single- and Double-Stranded DNA with Scalable Metamaterial-Based THz Biosensors
title_full_unstemmed Multifrequency Investigation of Single- and Double-Stranded DNA with Scalable Metamaterial-Based THz Biosensors
title_short Multifrequency Investigation of Single- and Double-Stranded DNA with Scalable Metamaterial-Based THz Biosensors
title_sort multifrequency investigation of single- and double-stranded dna with scalable metamaterial-based thz biosensors
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9312848/
https://www.ncbi.nlm.nih.gov/pubmed/35884286
http://dx.doi.org/10.3390/bios12070483
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