Cargando…

Simultaneous Visualization of MiRNA-221 and Caspase-3 in Cancer Cells for Investigating the Feasibility of MiRNA-Targeted Therapy with a Dual-Color Fluorescent Nanosensor

MiRNA-targeted therapy holds great promise for precision cancer therapy. It is important to investigate the effect of changes in miRNA expression on apoptosis in order to evaluate miRNA-targeted therapy and achieve personalized therapy. In this study, we designed a dual-color fluorescent nanosensor...

Descripción completa

Detalles Bibliográficos
Autores principales: Ren, Mingyao, Chen, Zhe, Ge, Chuandong, Hu, Wei, Wang, Nianxing, Yang, Limin, Luan, Mingming, Xu, Jing
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9312853/
https://www.ncbi.nlm.nih.gov/pubmed/35884247
http://dx.doi.org/10.3390/bios12070444
_version_ 1784753934813888512
author Ren, Mingyao
Chen, Zhe
Ge, Chuandong
Hu, Wei
Wang, Nianxing
Yang, Limin
Luan, Mingming
Xu, Jing
author_facet Ren, Mingyao
Chen, Zhe
Ge, Chuandong
Hu, Wei
Wang, Nianxing
Yang, Limin
Luan, Mingming
Xu, Jing
author_sort Ren, Mingyao
collection PubMed
description MiRNA-targeted therapy holds great promise for precision cancer therapy. It is important to investigate the effect of changes in miRNA expression on apoptosis in order to evaluate miRNA-targeted therapy and achieve personalized therapy. In this study, we designed a dual-color fluorescent nanosensor consisting of grapheme oxide modified with a molecular beacon and peptide. The nanosensor can simultaneously detect and image miRNA-221 and apoptotic protein caspase-3 in living cells. Intracellular experiments showed that the nanosensor could be successfully applied for in situ monitoring of the effect of miRNA-221 expression changes on apoptosis by dual-color imaging. The current strategy could provide new avenues for investigating the feasibility of miRNA-targeted therapy, screening new anti-cancer drugs targeting miRNA and developing personalized treatment plans.
format Online
Article
Text
id pubmed-9312853
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-93128532022-07-26 Simultaneous Visualization of MiRNA-221 and Caspase-3 in Cancer Cells for Investigating the Feasibility of MiRNA-Targeted Therapy with a Dual-Color Fluorescent Nanosensor Ren, Mingyao Chen, Zhe Ge, Chuandong Hu, Wei Wang, Nianxing Yang, Limin Luan, Mingming Xu, Jing Biosensors (Basel) Communication MiRNA-targeted therapy holds great promise for precision cancer therapy. It is important to investigate the effect of changes in miRNA expression on apoptosis in order to evaluate miRNA-targeted therapy and achieve personalized therapy. In this study, we designed a dual-color fluorescent nanosensor consisting of grapheme oxide modified with a molecular beacon and peptide. The nanosensor can simultaneously detect and image miRNA-221 and apoptotic protein caspase-3 in living cells. Intracellular experiments showed that the nanosensor could be successfully applied for in situ monitoring of the effect of miRNA-221 expression changes on apoptosis by dual-color imaging. The current strategy could provide new avenues for investigating the feasibility of miRNA-targeted therapy, screening new anti-cancer drugs targeting miRNA and developing personalized treatment plans. MDPI 2022-06-23 /pmc/articles/PMC9312853/ /pubmed/35884247 http://dx.doi.org/10.3390/bios12070444 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Communication
Ren, Mingyao
Chen, Zhe
Ge, Chuandong
Hu, Wei
Wang, Nianxing
Yang, Limin
Luan, Mingming
Xu, Jing
Simultaneous Visualization of MiRNA-221 and Caspase-3 in Cancer Cells for Investigating the Feasibility of MiRNA-Targeted Therapy with a Dual-Color Fluorescent Nanosensor
title Simultaneous Visualization of MiRNA-221 and Caspase-3 in Cancer Cells for Investigating the Feasibility of MiRNA-Targeted Therapy with a Dual-Color Fluorescent Nanosensor
title_full Simultaneous Visualization of MiRNA-221 and Caspase-3 in Cancer Cells for Investigating the Feasibility of MiRNA-Targeted Therapy with a Dual-Color Fluorescent Nanosensor
title_fullStr Simultaneous Visualization of MiRNA-221 and Caspase-3 in Cancer Cells for Investigating the Feasibility of MiRNA-Targeted Therapy with a Dual-Color Fluorescent Nanosensor
title_full_unstemmed Simultaneous Visualization of MiRNA-221 and Caspase-3 in Cancer Cells for Investigating the Feasibility of MiRNA-Targeted Therapy with a Dual-Color Fluorescent Nanosensor
title_short Simultaneous Visualization of MiRNA-221 and Caspase-3 in Cancer Cells for Investigating the Feasibility of MiRNA-Targeted Therapy with a Dual-Color Fluorescent Nanosensor
title_sort simultaneous visualization of mirna-221 and caspase-3 in cancer cells for investigating the feasibility of mirna-targeted therapy with a dual-color fluorescent nanosensor
topic Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9312853/
https://www.ncbi.nlm.nih.gov/pubmed/35884247
http://dx.doi.org/10.3390/bios12070444
work_keys_str_mv AT renmingyao simultaneousvisualizationofmirna221andcaspase3incancercellsforinvestigatingthefeasibilityofmirnatargetedtherapywithadualcolorfluorescentnanosensor
AT chenzhe simultaneousvisualizationofmirna221andcaspase3incancercellsforinvestigatingthefeasibilityofmirnatargetedtherapywithadualcolorfluorescentnanosensor
AT gechuandong simultaneousvisualizationofmirna221andcaspase3incancercellsforinvestigatingthefeasibilityofmirnatargetedtherapywithadualcolorfluorescentnanosensor
AT huwei simultaneousvisualizationofmirna221andcaspase3incancercellsforinvestigatingthefeasibilityofmirnatargetedtherapywithadualcolorfluorescentnanosensor
AT wangnianxing simultaneousvisualizationofmirna221andcaspase3incancercellsforinvestigatingthefeasibilityofmirnatargetedtherapywithadualcolorfluorescentnanosensor
AT yanglimin simultaneousvisualizationofmirna221andcaspase3incancercellsforinvestigatingthefeasibilityofmirnatargetedtherapywithadualcolorfluorescentnanosensor
AT luanmingming simultaneousvisualizationofmirna221andcaspase3incancercellsforinvestigatingthefeasibilityofmirnatargetedtherapywithadualcolorfluorescentnanosensor
AT xujing simultaneousvisualizationofmirna221andcaspase3incancercellsforinvestigatingthefeasibilityofmirnatargetedtherapywithadualcolorfluorescentnanosensor