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Prefab Hollow Glass Microsphere-Based Immunosensor with Liquid Crystal Sensitization for Acute Myocardial Infarction Biomarker Detection

Quantitative detection of cardiac troponin biomarkers in blood is an important method for clinical diagnosis of acute myocardial infarction (AMI). In this work, a whispering gallery mode (WGM) microcavity immunosensor based on a prefab hollow glass microsphere (HGMS) with liquid crystal (LC) sensiti...

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Autores principales: Niu, Panpan, Jiang, Junfeng, Liu, Kun, Wang, Shuang, Xu, Tianhua, Wang, Ziyihui, Wang, Tong, Zhang, Xuezhi, Ding, Zhenyang, Liu, Yize, Liu, Tiegen
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9312929/
https://www.ncbi.nlm.nih.gov/pubmed/35884242
http://dx.doi.org/10.3390/bios12070439
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author Niu, Panpan
Jiang, Junfeng
Liu, Kun
Wang, Shuang
Xu, Tianhua
Wang, Ziyihui
Wang, Tong
Zhang, Xuezhi
Ding, Zhenyang
Liu, Yize
Liu, Tiegen
author_facet Niu, Panpan
Jiang, Junfeng
Liu, Kun
Wang, Shuang
Xu, Tianhua
Wang, Ziyihui
Wang, Tong
Zhang, Xuezhi
Ding, Zhenyang
Liu, Yize
Liu, Tiegen
author_sort Niu, Panpan
collection PubMed
description Quantitative detection of cardiac troponin biomarkers in blood is an important method for clinical diagnosis of acute myocardial infarction (AMI). In this work, a whispering gallery mode (WGM) microcavity immunosensor based on a prefab hollow glass microsphere (HGMS) with liquid crystal (LC) sensitization was proposed and experimentally demonstrated for label-free cardiac troponin I-C (cTnI-C) complex detection. The proposed fiber-optic immunosensor has a simple structure; the tiny modified HGMS serves as the key sensing element and the microsample reservoir simultaneously. A sensitive LC layer with cTnI-C recognition ability was deposited on the inner wall of the HGMS microcavity. The arrangement of LC molecules is affected by the cTnI-C antigen—antibody binding in the HGMS, and the small change of the surface refractive index caused by the binding can be amplified owing to the birefringence property of LC. Using the annular waveguide of the HGMS, the WGMs were easily excited by the coupling scanning laser with a microfiber, and an all-fiber cTnI-C immunosensor can be achieved by measuring the resonant wavelength shift of the WGM spectrum. Moreover, the dynamic processes of the cTnI-C antigen—antibody binding and unbinding was revealed by monitoring the wavelength shift continuously. The proposed immunosensor with a spherical microcavity can be a cost-effective tool for AMI diagnosis.
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spelling pubmed-93129292022-07-26 Prefab Hollow Glass Microsphere-Based Immunosensor with Liquid Crystal Sensitization for Acute Myocardial Infarction Biomarker Detection Niu, Panpan Jiang, Junfeng Liu, Kun Wang, Shuang Xu, Tianhua Wang, Ziyihui Wang, Tong Zhang, Xuezhi Ding, Zhenyang Liu, Yize Liu, Tiegen Biosensors (Basel) Article Quantitative detection of cardiac troponin biomarkers in blood is an important method for clinical diagnosis of acute myocardial infarction (AMI). In this work, a whispering gallery mode (WGM) microcavity immunosensor based on a prefab hollow glass microsphere (HGMS) with liquid crystal (LC) sensitization was proposed and experimentally demonstrated for label-free cardiac troponin I-C (cTnI-C) complex detection. The proposed fiber-optic immunosensor has a simple structure; the tiny modified HGMS serves as the key sensing element and the microsample reservoir simultaneously. A sensitive LC layer with cTnI-C recognition ability was deposited on the inner wall of the HGMS microcavity. The arrangement of LC molecules is affected by the cTnI-C antigen—antibody binding in the HGMS, and the small change of the surface refractive index caused by the binding can be amplified owing to the birefringence property of LC. Using the annular waveguide of the HGMS, the WGMs were easily excited by the coupling scanning laser with a microfiber, and an all-fiber cTnI-C immunosensor can be achieved by measuring the resonant wavelength shift of the WGM spectrum. Moreover, the dynamic processes of the cTnI-C antigen—antibody binding and unbinding was revealed by monitoring the wavelength shift continuously. The proposed immunosensor with a spherical microcavity can be a cost-effective tool for AMI diagnosis. MDPI 2022-06-22 /pmc/articles/PMC9312929/ /pubmed/35884242 http://dx.doi.org/10.3390/bios12070439 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Niu, Panpan
Jiang, Junfeng
Liu, Kun
Wang, Shuang
Xu, Tianhua
Wang, Ziyihui
Wang, Tong
Zhang, Xuezhi
Ding, Zhenyang
Liu, Yize
Liu, Tiegen
Prefab Hollow Glass Microsphere-Based Immunosensor with Liquid Crystal Sensitization for Acute Myocardial Infarction Biomarker Detection
title Prefab Hollow Glass Microsphere-Based Immunosensor with Liquid Crystal Sensitization for Acute Myocardial Infarction Biomarker Detection
title_full Prefab Hollow Glass Microsphere-Based Immunosensor with Liquid Crystal Sensitization for Acute Myocardial Infarction Biomarker Detection
title_fullStr Prefab Hollow Glass Microsphere-Based Immunosensor with Liquid Crystal Sensitization for Acute Myocardial Infarction Biomarker Detection
title_full_unstemmed Prefab Hollow Glass Microsphere-Based Immunosensor with Liquid Crystal Sensitization for Acute Myocardial Infarction Biomarker Detection
title_short Prefab Hollow Glass Microsphere-Based Immunosensor with Liquid Crystal Sensitization for Acute Myocardial Infarction Biomarker Detection
title_sort prefab hollow glass microsphere-based immunosensor with liquid crystal sensitization for acute myocardial infarction biomarker detection
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9312929/
https://www.ncbi.nlm.nih.gov/pubmed/35884242
http://dx.doi.org/10.3390/bios12070439
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