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Signal-Based Methods in Dielectrophoresis for Cell and Particle Separation
Separation and detection of cells and particles in a suspension are essential for various applications, including biomedical investigations and clinical diagnostics. Microfluidics realizes the miniaturization of analytical devices by controlling the motion of a small volume of fluids in microchannel...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9313092/ https://www.ncbi.nlm.nih.gov/pubmed/35884313 http://dx.doi.org/10.3390/bios12070510 |
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author | Farasat, Malihe Aalaei, Ehsan Kheirati Ronizi, Saeed Bakhshi, Atin Mirhosseini, Shaghayegh Zhang, Jun Nguyen, Nam-Trung Kashaninejad, Navid |
author_facet | Farasat, Malihe Aalaei, Ehsan Kheirati Ronizi, Saeed Bakhshi, Atin Mirhosseini, Shaghayegh Zhang, Jun Nguyen, Nam-Trung Kashaninejad, Navid |
author_sort | Farasat, Malihe |
collection | PubMed |
description | Separation and detection of cells and particles in a suspension are essential for various applications, including biomedical investigations and clinical diagnostics. Microfluidics realizes the miniaturization of analytical devices by controlling the motion of a small volume of fluids in microchannels and microchambers. Accordingly, microfluidic devices have been widely used in particle/cell manipulation processes. Different microfluidic methods for particle separation include dielectrophoretic, magnetic, optical, acoustic, hydrodynamic, and chemical techniques. Dielectrophoresis (DEP) is a method for manipulating polarizable particles’ trajectories in non-uniform electric fields using unique dielectric characteristics. It provides several advantages for dealing with neutral bioparticles owing to its sensitivity, selectivity, and noninvasive nature. This review provides a detailed study on the signal-based DEP methods that use the applied signal parameters, including frequency, amplitude, phase, and shape for cell/particle separation and manipulation. Rather than employing complex channels or time-consuming fabrication procedures, these methods realize sorting and detecting the cells/particles by modifying the signal parameters while using a relatively simple device. In addition, these methods can significantly impact clinical diagnostics by making low-cost and rapid separation possible. We conclude the review by discussing the technical and biological challenges of DEP techniques and providing future perspectives in this field. |
format | Online Article Text |
id | pubmed-9313092 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-93130922022-07-26 Signal-Based Methods in Dielectrophoresis for Cell and Particle Separation Farasat, Malihe Aalaei, Ehsan Kheirati Ronizi, Saeed Bakhshi, Atin Mirhosseini, Shaghayegh Zhang, Jun Nguyen, Nam-Trung Kashaninejad, Navid Biosensors (Basel) Review Separation and detection of cells and particles in a suspension are essential for various applications, including biomedical investigations and clinical diagnostics. Microfluidics realizes the miniaturization of analytical devices by controlling the motion of a small volume of fluids in microchannels and microchambers. Accordingly, microfluidic devices have been widely used in particle/cell manipulation processes. Different microfluidic methods for particle separation include dielectrophoretic, magnetic, optical, acoustic, hydrodynamic, and chemical techniques. Dielectrophoresis (DEP) is a method for manipulating polarizable particles’ trajectories in non-uniform electric fields using unique dielectric characteristics. It provides several advantages for dealing with neutral bioparticles owing to its sensitivity, selectivity, and noninvasive nature. This review provides a detailed study on the signal-based DEP methods that use the applied signal parameters, including frequency, amplitude, phase, and shape for cell/particle separation and manipulation. Rather than employing complex channels or time-consuming fabrication procedures, these methods realize sorting and detecting the cells/particles by modifying the signal parameters while using a relatively simple device. In addition, these methods can significantly impact clinical diagnostics by making low-cost and rapid separation possible. We conclude the review by discussing the technical and biological challenges of DEP techniques and providing future perspectives in this field. MDPI 2022-07-11 /pmc/articles/PMC9313092/ /pubmed/35884313 http://dx.doi.org/10.3390/bios12070510 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Review Farasat, Malihe Aalaei, Ehsan Kheirati Ronizi, Saeed Bakhshi, Atin Mirhosseini, Shaghayegh Zhang, Jun Nguyen, Nam-Trung Kashaninejad, Navid Signal-Based Methods in Dielectrophoresis for Cell and Particle Separation |
title | Signal-Based Methods in Dielectrophoresis for Cell and Particle Separation |
title_full | Signal-Based Methods in Dielectrophoresis for Cell and Particle Separation |
title_fullStr | Signal-Based Methods in Dielectrophoresis for Cell and Particle Separation |
title_full_unstemmed | Signal-Based Methods in Dielectrophoresis for Cell and Particle Separation |
title_short | Signal-Based Methods in Dielectrophoresis for Cell and Particle Separation |
title_sort | signal-based methods in dielectrophoresis for cell and particle separation |
topic | Review |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9313092/ https://www.ncbi.nlm.nih.gov/pubmed/35884313 http://dx.doi.org/10.3390/bios12070510 |
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