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New Microfluidic System for Electrochemical Impedance Spectroscopy Assessment of Cell Culture Performance: Design and Development of New Electrode Material

Electrochemical impedance spectroscopy (EIS) is widely accepted as an effective and non-destructive method to assess cell health during cell-culture. However, there is a lack of compact devices compatible with microfluidic integration and microscopy that could provide the real-time and non-invasive...

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Autores principales: Chmayssem, Ayman, Tanase, Constantin Edi, Verplanck, Nicolas, Gougis, Maxime, Mourier, Véronique, Zebda, Abdelkader, Ghaemmaghami, Amir M., Mailley, Pascal
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9313146/
https://www.ncbi.nlm.nih.gov/pubmed/35884254
http://dx.doi.org/10.3390/bios12070452
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author Chmayssem, Ayman
Tanase, Constantin Edi
Verplanck, Nicolas
Gougis, Maxime
Mourier, Véronique
Zebda, Abdelkader
Ghaemmaghami, Amir M.
Mailley, Pascal
author_facet Chmayssem, Ayman
Tanase, Constantin Edi
Verplanck, Nicolas
Gougis, Maxime
Mourier, Véronique
Zebda, Abdelkader
Ghaemmaghami, Amir M.
Mailley, Pascal
author_sort Chmayssem, Ayman
collection PubMed
description Electrochemical impedance spectroscopy (EIS) is widely accepted as an effective and non-destructive method to assess cell health during cell-culture. However, there is a lack of compact devices compatible with microfluidic integration and microscopy that could provide the real-time and non-invasive monitoring of cell-cultures using EIS. In this paper, we reported the design and characterization of a modular EIS testing system based on a patented technology. This device was fabricated using easily processable methodologies including screen-printing of the impedance electrodes and molding or micromachining of the cell culture chamber with an easy assembly procedure. Accordingly, to obtain processable, biocompatible and sterilizable electrode materials that lower the impact of interfacial impedance on TEER (Transepithelial electrical resistance) measurements, and to enable concomitant microscopy observations, we optimized the formulation of the electrode inks and the design of the EIS electrodes, respectively. First, electrode materials were based on carbon biocompatible inks enriched with IrOx particles to obtain low interfacial impedance electrodes approaching the performances of classical non-biocompatible Ag/AgCl second-species electrodes. Secondly, we proposed three original electrode designs, which were compared to classical disk electrodes that were optically compatible with microscopy. We assessed the impact of the electrode design on the response of the impedance sensor using COMSOL Multiphysics. Finally, the performance of the impedance spectroscopy devices was assessed in vitro using human airway epithelial cell cultures.
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spelling pubmed-93131462022-07-26 New Microfluidic System for Electrochemical Impedance Spectroscopy Assessment of Cell Culture Performance: Design and Development of New Electrode Material Chmayssem, Ayman Tanase, Constantin Edi Verplanck, Nicolas Gougis, Maxime Mourier, Véronique Zebda, Abdelkader Ghaemmaghami, Amir M. Mailley, Pascal Biosensors (Basel) Article Electrochemical impedance spectroscopy (EIS) is widely accepted as an effective and non-destructive method to assess cell health during cell-culture. However, there is a lack of compact devices compatible with microfluidic integration and microscopy that could provide the real-time and non-invasive monitoring of cell-cultures using EIS. In this paper, we reported the design and characterization of a modular EIS testing system based on a patented technology. This device was fabricated using easily processable methodologies including screen-printing of the impedance electrodes and molding or micromachining of the cell culture chamber with an easy assembly procedure. Accordingly, to obtain processable, biocompatible and sterilizable electrode materials that lower the impact of interfacial impedance on TEER (Transepithelial electrical resistance) measurements, and to enable concomitant microscopy observations, we optimized the formulation of the electrode inks and the design of the EIS electrodes, respectively. First, electrode materials were based on carbon biocompatible inks enriched with IrOx particles to obtain low interfacial impedance electrodes approaching the performances of classical non-biocompatible Ag/AgCl second-species electrodes. Secondly, we proposed three original electrode designs, which were compared to classical disk electrodes that were optically compatible with microscopy. We assessed the impact of the electrode design on the response of the impedance sensor using COMSOL Multiphysics. Finally, the performance of the impedance spectroscopy devices was assessed in vitro using human airway epithelial cell cultures. MDPI 2022-06-24 /pmc/articles/PMC9313146/ /pubmed/35884254 http://dx.doi.org/10.3390/bios12070452 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Chmayssem, Ayman
Tanase, Constantin Edi
Verplanck, Nicolas
Gougis, Maxime
Mourier, Véronique
Zebda, Abdelkader
Ghaemmaghami, Amir M.
Mailley, Pascal
New Microfluidic System for Electrochemical Impedance Spectroscopy Assessment of Cell Culture Performance: Design and Development of New Electrode Material
title New Microfluidic System for Electrochemical Impedance Spectroscopy Assessment of Cell Culture Performance: Design and Development of New Electrode Material
title_full New Microfluidic System for Electrochemical Impedance Spectroscopy Assessment of Cell Culture Performance: Design and Development of New Electrode Material
title_fullStr New Microfluidic System for Electrochemical Impedance Spectroscopy Assessment of Cell Culture Performance: Design and Development of New Electrode Material
title_full_unstemmed New Microfluidic System for Electrochemical Impedance Spectroscopy Assessment of Cell Culture Performance: Design and Development of New Electrode Material
title_short New Microfluidic System for Electrochemical Impedance Spectroscopy Assessment of Cell Culture Performance: Design and Development of New Electrode Material
title_sort new microfluidic system for electrochemical impedance spectroscopy assessment of cell culture performance: design and development of new electrode material
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9313146/
https://www.ncbi.nlm.nih.gov/pubmed/35884254
http://dx.doi.org/10.3390/bios12070452
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