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The Antibacterial Effect of Platelets on Escherichia coli Strains
Platelets play an important role in defense against pathogens; however, the interaction between Escherichia coli and platelets has not been well described and detailed. Our goal was to study the interaction between platelets and selected strains of E. coli in order to evaluate the antibacterial effe...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9313237/ https://www.ncbi.nlm.nih.gov/pubmed/35884840 http://dx.doi.org/10.3390/biomedicines10071533 |
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author | Ezzeroug Ezzraimi, Amina Hannachi, Nadji Mariotti, Antoine Rolland, Clara Levasseur, Anthony Baron, Sophie Alexandra Rolain, Jean-Marc Camoin-Jau, Laurence |
author_facet | Ezzeroug Ezzraimi, Amina Hannachi, Nadji Mariotti, Antoine Rolland, Clara Levasseur, Anthony Baron, Sophie Alexandra Rolain, Jean-Marc Camoin-Jau, Laurence |
author_sort | Ezzeroug Ezzraimi, Amina |
collection | PubMed |
description | Platelets play an important role in defense against pathogens; however, the interaction between Escherichia coli and platelets has not been well described and detailed. Our goal was to study the interaction between platelets and selected strains of E. coli in order to evaluate the antibacterial effect of platelets and to assess bacterial effects on platelet activation. Washed platelets and supernatants of pre-activated platelets were incubated with five clinical colistin-resistant and five laboratory colistin-sensitive strains of E. coli in order to study bacterial growth. Platelet activation was measured with flow cytometry by evaluating CD62P expression. To identify the difference in strain behavior toward platelets, a pangenome analysis using Roary and O-antigen serotyping was carried out. Both whole platelets and the supernatant of activated platelets inhibited growth of three laboratory colistin-sensitive strains. In contrast, platelets promoted growth of the other strains. There was a negative correlation between platelet activation and bacterial growth. The Roary results showed no logical clustering to explain the mechanism of platelet resistance. The diversity of the responses might be due to strains of different types of O-antigen. Our results show a bidirectional interaction between platelets and E. coli whose expression is dependent on the bacterial strain involved. |
format | Online Article Text |
id | pubmed-9313237 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-93132372022-07-26 The Antibacterial Effect of Platelets on Escherichia coli Strains Ezzeroug Ezzraimi, Amina Hannachi, Nadji Mariotti, Antoine Rolland, Clara Levasseur, Anthony Baron, Sophie Alexandra Rolain, Jean-Marc Camoin-Jau, Laurence Biomedicines Article Platelets play an important role in defense against pathogens; however, the interaction between Escherichia coli and platelets has not been well described and detailed. Our goal was to study the interaction between platelets and selected strains of E. coli in order to evaluate the antibacterial effect of platelets and to assess bacterial effects on platelet activation. Washed platelets and supernatants of pre-activated platelets were incubated with five clinical colistin-resistant and five laboratory colistin-sensitive strains of E. coli in order to study bacterial growth. Platelet activation was measured with flow cytometry by evaluating CD62P expression. To identify the difference in strain behavior toward platelets, a pangenome analysis using Roary and O-antigen serotyping was carried out. Both whole platelets and the supernatant of activated platelets inhibited growth of three laboratory colistin-sensitive strains. In contrast, platelets promoted growth of the other strains. There was a negative correlation between platelet activation and bacterial growth. The Roary results showed no logical clustering to explain the mechanism of platelet resistance. The diversity of the responses might be due to strains of different types of O-antigen. Our results show a bidirectional interaction between platelets and E. coli whose expression is dependent on the bacterial strain involved. MDPI 2022-06-28 /pmc/articles/PMC9313237/ /pubmed/35884840 http://dx.doi.org/10.3390/biomedicines10071533 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Ezzeroug Ezzraimi, Amina Hannachi, Nadji Mariotti, Antoine Rolland, Clara Levasseur, Anthony Baron, Sophie Alexandra Rolain, Jean-Marc Camoin-Jau, Laurence The Antibacterial Effect of Platelets on Escherichia coli Strains |
title | The Antibacterial Effect of Platelets on Escherichia coli Strains |
title_full | The Antibacterial Effect of Platelets on Escherichia coli Strains |
title_fullStr | The Antibacterial Effect of Platelets on Escherichia coli Strains |
title_full_unstemmed | The Antibacterial Effect of Platelets on Escherichia coli Strains |
title_short | The Antibacterial Effect of Platelets on Escherichia coli Strains |
title_sort | antibacterial effect of platelets on escherichia coli strains |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9313237/ https://www.ncbi.nlm.nih.gov/pubmed/35884840 http://dx.doi.org/10.3390/biomedicines10071533 |
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