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Development of a Biotechnology Platform for the Fast-Growing Cyanobacterium Synechococcus sp. PCC 11901

Synechococcus sp. PCC 11901 reportedly demonstrates the highest, most sustained growth of any known cyanobacterium under optimized conditions. Due to its recent discovery, our knowledge of its biology, including the factors underlying sustained, fast growth, is limited. Furthermore, tools specific f...

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Autores principales: Mills, Lauren A., Moreno-Cabezuelo, José Ángel, Włodarczyk, Artur, Victoria, Angelo J., Mejías, Rebeca, Nenninger, Anja, Moxon, Simon, Bombelli, Paolo, Selão, Tiago T., McCormick, Alistair J., Lea-Smith, David J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9313322/
https://www.ncbi.nlm.nih.gov/pubmed/35883428
http://dx.doi.org/10.3390/biom12070872
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author Mills, Lauren A.
Moreno-Cabezuelo, José Ángel
Włodarczyk, Artur
Victoria, Angelo J.
Mejías, Rebeca
Nenninger, Anja
Moxon, Simon
Bombelli, Paolo
Selão, Tiago T.
McCormick, Alistair J.
Lea-Smith, David J.
author_facet Mills, Lauren A.
Moreno-Cabezuelo, José Ángel
Włodarczyk, Artur
Victoria, Angelo J.
Mejías, Rebeca
Nenninger, Anja
Moxon, Simon
Bombelli, Paolo
Selão, Tiago T.
McCormick, Alistair J.
Lea-Smith, David J.
author_sort Mills, Lauren A.
collection PubMed
description Synechococcus sp. PCC 11901 reportedly demonstrates the highest, most sustained growth of any known cyanobacterium under optimized conditions. Due to its recent discovery, our knowledge of its biology, including the factors underlying sustained, fast growth, is limited. Furthermore, tools specific for genetic manipulation of PCC 11901 are not established. Here, we demonstrate that PCC 11901 shows faster growth than other model cyanobacteria, including the fast-growing species Synechococcus elongatus UTEX 2973, under optimal growth conditions for UTEX 2973. Comparative genomics between PCC 11901 and Synechocystis sp. PCC 6803 reveal conservation of most metabolic pathways but PCC 11901 has a simplified electron transport chain and reduced light harvesting complex. This may underlie its superior light use, reduced photoinhibition, and higher photosynthetic and respiratory rates. To aid biotechnology applications, we developed a vitamin B(12) auxotrophic mutant but were unable to generate unmarked knockouts using two negative selectable markers, suggesting that recombinase- or CRISPR-based approaches may be required for repeated genetic manipulation. Overall, this study establishes PCC 11901 as one of the most promising species currently available for cyanobacterial biotechnology and provides a useful set of bioinformatics tools and strains for advancing this field, in addition to insights into the factors underlying its fast growth phenotype.
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spelling pubmed-93133222022-07-26 Development of a Biotechnology Platform for the Fast-Growing Cyanobacterium Synechococcus sp. PCC 11901 Mills, Lauren A. Moreno-Cabezuelo, José Ángel Włodarczyk, Artur Victoria, Angelo J. Mejías, Rebeca Nenninger, Anja Moxon, Simon Bombelli, Paolo Selão, Tiago T. McCormick, Alistair J. Lea-Smith, David J. Biomolecules Article Synechococcus sp. PCC 11901 reportedly demonstrates the highest, most sustained growth of any known cyanobacterium under optimized conditions. Due to its recent discovery, our knowledge of its biology, including the factors underlying sustained, fast growth, is limited. Furthermore, tools specific for genetic manipulation of PCC 11901 are not established. Here, we demonstrate that PCC 11901 shows faster growth than other model cyanobacteria, including the fast-growing species Synechococcus elongatus UTEX 2973, under optimal growth conditions for UTEX 2973. Comparative genomics between PCC 11901 and Synechocystis sp. PCC 6803 reveal conservation of most metabolic pathways but PCC 11901 has a simplified electron transport chain and reduced light harvesting complex. This may underlie its superior light use, reduced photoinhibition, and higher photosynthetic and respiratory rates. To aid biotechnology applications, we developed a vitamin B(12) auxotrophic mutant but were unable to generate unmarked knockouts using two negative selectable markers, suggesting that recombinase- or CRISPR-based approaches may be required for repeated genetic manipulation. Overall, this study establishes PCC 11901 as one of the most promising species currently available for cyanobacterial biotechnology and provides a useful set of bioinformatics tools and strains for advancing this field, in addition to insights into the factors underlying its fast growth phenotype. MDPI 2022-06-23 /pmc/articles/PMC9313322/ /pubmed/35883428 http://dx.doi.org/10.3390/biom12070872 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Mills, Lauren A.
Moreno-Cabezuelo, José Ángel
Włodarczyk, Artur
Victoria, Angelo J.
Mejías, Rebeca
Nenninger, Anja
Moxon, Simon
Bombelli, Paolo
Selão, Tiago T.
McCormick, Alistair J.
Lea-Smith, David J.
Development of a Biotechnology Platform for the Fast-Growing Cyanobacterium Synechococcus sp. PCC 11901
title Development of a Biotechnology Platform for the Fast-Growing Cyanobacterium Synechococcus sp. PCC 11901
title_full Development of a Biotechnology Platform for the Fast-Growing Cyanobacterium Synechococcus sp. PCC 11901
title_fullStr Development of a Biotechnology Platform for the Fast-Growing Cyanobacterium Synechococcus sp. PCC 11901
title_full_unstemmed Development of a Biotechnology Platform for the Fast-Growing Cyanobacterium Synechococcus sp. PCC 11901
title_short Development of a Biotechnology Platform for the Fast-Growing Cyanobacterium Synechococcus sp. PCC 11901
title_sort development of a biotechnology platform for the fast-growing cyanobacterium synechococcus sp. pcc 11901
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9313322/
https://www.ncbi.nlm.nih.gov/pubmed/35883428
http://dx.doi.org/10.3390/biom12070872
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