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All-in-One Optofluidic Chip for Molecular Biosensing Assays

Integrated biosensor platforms have become subjects of high interest for consolidated assay preparation and analysis to reduce sample-to-answer response times. By compactly combining as many biosensor processes and functions as possible into a single lab-on-chip device, all-in-one point-of-care devi...

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Autores principales: Sano, Tyler, Zhang, Han, Losakul, Ravipa, Schmidt, Holger
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9313335/
https://www.ncbi.nlm.nih.gov/pubmed/35884304
http://dx.doi.org/10.3390/bios12070501
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author Sano, Tyler
Zhang, Han
Losakul, Ravipa
Schmidt, Holger
author_facet Sano, Tyler
Zhang, Han
Losakul, Ravipa
Schmidt, Holger
author_sort Sano, Tyler
collection PubMed
description Integrated biosensor platforms have become subjects of high interest for consolidated assay preparation and analysis to reduce sample-to-answer response times. By compactly combining as many biosensor processes and functions as possible into a single lab-on-chip device, all-in-one point-of-care devices can aid in the accessibility and speed of deployment due to their compact size and portability. Biomarker assay preparation and sensing are functionalities that are often carried out on separate devices, thus increasing opportunity of contamination, loss of sample volume, and other forms of error. Here, we demonstrate a complete lab-on-chip system combining sample preparation, on-chip optofluidic dye laser, and optical detection. We first show the integration of an on-chip distributed feedback dye laser for alignment-free optical excitation of particles moving through a fluidic channel. This capability is demonstrated by using Rhodamine 6G as the gain medium to excite single fluorescent microspheres at 575 nm. Next, we present an optofluidic PDMS platform combining a microvalve network (automaton) for sample preparation of nanoliter volumes, on-chip distributed feedback dye laser for target excitation, and optical detection. We conduct concurrent capture and fluorescence tagging of Zika virus nucleic acid on magnetic beads in 30 min. Target-carrying beads are then optically excited using the on-chip laser as they flow through an analysis channel, followed by highly specific fluorescence detection. This demonstration of a complete all-in-one biosensor is a tangible step in the development of a rapid, point-of-care device that can assist in limiting the severity of future outbreaks.
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spelling pubmed-93133352022-07-26 All-in-One Optofluidic Chip for Molecular Biosensing Assays Sano, Tyler Zhang, Han Losakul, Ravipa Schmidt, Holger Biosensors (Basel) Article Integrated biosensor platforms have become subjects of high interest for consolidated assay preparation and analysis to reduce sample-to-answer response times. By compactly combining as many biosensor processes and functions as possible into a single lab-on-chip device, all-in-one point-of-care devices can aid in the accessibility and speed of deployment due to their compact size and portability. Biomarker assay preparation and sensing are functionalities that are often carried out on separate devices, thus increasing opportunity of contamination, loss of sample volume, and other forms of error. Here, we demonstrate a complete lab-on-chip system combining sample preparation, on-chip optofluidic dye laser, and optical detection. We first show the integration of an on-chip distributed feedback dye laser for alignment-free optical excitation of particles moving through a fluidic channel. This capability is demonstrated by using Rhodamine 6G as the gain medium to excite single fluorescent microspheres at 575 nm. Next, we present an optofluidic PDMS platform combining a microvalve network (automaton) for sample preparation of nanoliter volumes, on-chip distributed feedback dye laser for target excitation, and optical detection. We conduct concurrent capture and fluorescence tagging of Zika virus nucleic acid on magnetic beads in 30 min. Target-carrying beads are then optically excited using the on-chip laser as they flow through an analysis channel, followed by highly specific fluorescence detection. This demonstration of a complete all-in-one biosensor is a tangible step in the development of a rapid, point-of-care device that can assist in limiting the severity of future outbreaks. MDPI 2022-07-09 /pmc/articles/PMC9313335/ /pubmed/35884304 http://dx.doi.org/10.3390/bios12070501 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Sano, Tyler
Zhang, Han
Losakul, Ravipa
Schmidt, Holger
All-in-One Optofluidic Chip for Molecular Biosensing Assays
title All-in-One Optofluidic Chip for Molecular Biosensing Assays
title_full All-in-One Optofluidic Chip for Molecular Biosensing Assays
title_fullStr All-in-One Optofluidic Chip for Molecular Biosensing Assays
title_full_unstemmed All-in-One Optofluidic Chip for Molecular Biosensing Assays
title_short All-in-One Optofluidic Chip for Molecular Biosensing Assays
title_sort all-in-one optofluidic chip for molecular biosensing assays
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9313335/
https://www.ncbi.nlm.nih.gov/pubmed/35884304
http://dx.doi.org/10.3390/bios12070501
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AT schmidtholger allinoneoptofluidicchipformolecularbiosensingassays