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3D Bioprinting of Multi-Material Decellularized Liver Matrix Hydrogel at Physiological Temperatures
Bioprinting is an acclaimed technique that allows the scaling of 3D architectures in an organized pattern but suffers from a scarcity of appropriate bioinks. Decellularized extracellular matrix (dECM) from xenogeneic species has garnered support as a biomaterial to promote tissue-specific regenerati...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9313433/ https://www.ncbi.nlm.nih.gov/pubmed/35884324 http://dx.doi.org/10.3390/bios12070521 |
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author | Khati, Vamakshi Ramachandraiah, Harisha Pati, Falguni Svahn, Helene A. Gaudenzi, Giulia Russom, Aman |
author_facet | Khati, Vamakshi Ramachandraiah, Harisha Pati, Falguni Svahn, Helene A. Gaudenzi, Giulia Russom, Aman |
author_sort | Khati, Vamakshi |
collection | PubMed |
description | Bioprinting is an acclaimed technique that allows the scaling of 3D architectures in an organized pattern but suffers from a scarcity of appropriate bioinks. Decellularized extracellular matrix (dECM) from xenogeneic species has garnered support as a biomaterial to promote tissue-specific regeneration and repair. The prospect of developing dECM-based 3D artificial tissue is impeded by its inherent low mechanical properties. In recent years, 3D bioprinting of dECM-based bioinks modified with additional scaffolds has advanced the development of load-bearing constructs. However, previous attempts using dECM were limited to low-temperature bioprinting, which is not favorable for a longer print duration with cells. Here, we report the development of a multi-material decellularized liver matrix (dLM) bioink reinforced with gelatin and polyethylene glycol to improve rheology, extrudability, and mechanical stability. This shear-thinning bioink facilitated extrusion-based bioprinting at 37 °C with HepG2 cells into a 3D grid structure with a further enhancement for long-term applications by enzymatic crosslinking with mushroom tyrosinase. The heavily crosslinked structure showed a 16-fold increase in viscosity (2.73 Pa s(−1)) and a 32-fold increase in storage modulus from the non-crosslinked dLM while retaining high cell viability (85–93%) and liver-specific functions. Our results show that the cytocompatible crosslinking of dLM bioink at physiological temperatures has promising applications for extended 3D-printing procedures. |
format | Online Article Text |
id | pubmed-9313433 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-93134332022-07-26 3D Bioprinting of Multi-Material Decellularized Liver Matrix Hydrogel at Physiological Temperatures Khati, Vamakshi Ramachandraiah, Harisha Pati, Falguni Svahn, Helene A. Gaudenzi, Giulia Russom, Aman Biosensors (Basel) Article Bioprinting is an acclaimed technique that allows the scaling of 3D architectures in an organized pattern but suffers from a scarcity of appropriate bioinks. Decellularized extracellular matrix (dECM) from xenogeneic species has garnered support as a biomaterial to promote tissue-specific regeneration and repair. The prospect of developing dECM-based 3D artificial tissue is impeded by its inherent low mechanical properties. In recent years, 3D bioprinting of dECM-based bioinks modified with additional scaffolds has advanced the development of load-bearing constructs. However, previous attempts using dECM were limited to low-temperature bioprinting, which is not favorable for a longer print duration with cells. Here, we report the development of a multi-material decellularized liver matrix (dLM) bioink reinforced with gelatin and polyethylene glycol to improve rheology, extrudability, and mechanical stability. This shear-thinning bioink facilitated extrusion-based bioprinting at 37 °C with HepG2 cells into a 3D grid structure with a further enhancement for long-term applications by enzymatic crosslinking with mushroom tyrosinase. The heavily crosslinked structure showed a 16-fold increase in viscosity (2.73 Pa s(−1)) and a 32-fold increase in storage modulus from the non-crosslinked dLM while retaining high cell viability (85–93%) and liver-specific functions. Our results show that the cytocompatible crosslinking of dLM bioink at physiological temperatures has promising applications for extended 3D-printing procedures. MDPI 2022-07-13 /pmc/articles/PMC9313433/ /pubmed/35884324 http://dx.doi.org/10.3390/bios12070521 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Khati, Vamakshi Ramachandraiah, Harisha Pati, Falguni Svahn, Helene A. Gaudenzi, Giulia Russom, Aman 3D Bioprinting of Multi-Material Decellularized Liver Matrix Hydrogel at Physiological Temperatures |
title | 3D Bioprinting of Multi-Material Decellularized Liver Matrix Hydrogel at Physiological Temperatures |
title_full | 3D Bioprinting of Multi-Material Decellularized Liver Matrix Hydrogel at Physiological Temperatures |
title_fullStr | 3D Bioprinting of Multi-Material Decellularized Liver Matrix Hydrogel at Physiological Temperatures |
title_full_unstemmed | 3D Bioprinting of Multi-Material Decellularized Liver Matrix Hydrogel at Physiological Temperatures |
title_short | 3D Bioprinting of Multi-Material Decellularized Liver Matrix Hydrogel at Physiological Temperatures |
title_sort | 3d bioprinting of multi-material decellularized liver matrix hydrogel at physiological temperatures |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9313433/ https://www.ncbi.nlm.nih.gov/pubmed/35884324 http://dx.doi.org/10.3390/bios12070521 |
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