Cargando…

Genome-Wide Identification of MAPK, MAPKK, and MAPKKK Gene Families in Fagopyrum tataricum and Analysis of Their Expression Patterns Under Abiotic Stress

The mitogen-activated protein kinase (MAPK) cascade is a highly conserved signal transduction pathway, ubiquitous in eukaryotes, such as animals and plants. The MAPK cascade has a dominant role in regulating plant adaptation to the environment, such as through stress responses, osmotic adjustment, a...

Descripción completa

Detalles Bibliográficos
Autores principales: Wang, Zhen, Yan, Song, Ren, Weichao, Liu, Yan, Sun, Wei, Liu, Meiqi, Lu, Jiaxin, Mi, Yaolei, Ma, Wei
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9313540/
https://www.ncbi.nlm.nih.gov/pubmed/35899198
http://dx.doi.org/10.3389/fgene.2022.894048
_version_ 1784754104335073280
author Wang, Zhen
Yan, Song
Ren, Weichao
Liu, Yan
Sun, Wei
Liu, Meiqi
Lu, Jiaxin
Mi, Yaolei
Ma, Wei
author_facet Wang, Zhen
Yan, Song
Ren, Weichao
Liu, Yan
Sun, Wei
Liu, Meiqi
Lu, Jiaxin
Mi, Yaolei
Ma, Wei
author_sort Wang, Zhen
collection PubMed
description The mitogen-activated protein kinase (MAPK) cascade is a highly conserved signal transduction pathway, ubiquitous in eukaryotes, such as animals and plants. The MAPK cascade has a dominant role in regulating plant adaptation to the environment, such as through stress responses, osmotic adjustment, and processes that modulate pathogenicity. In the present study, the MAPK cascade gene family was identified in Fagopyrum tataricum (Tartary buckwheat), based on complete genome sequence data. Using phylogenetic tree, conservative motif, and chromosome location analyses, a total of 65 FtMAPK cascade genes, distributed on five chromosomes, were classified into three families: MAPK (n = 8), MAPKK (n = 1), and MAPKKK (n = 56). Transcriptome data from Tartary buckwheat seedlings grown under different light conditions demonstrated that, under blue and red light, the expression levels of 18 and 36 FtMAPK cascade genes were up-regulated and down-regulated, respectively. Through qRT-PCR experiments, it was observed that FtMAPK5, FtMAPKK1, FtMAPKKK8, FtMAPKKK10, and FtMAPKKK24 gene expression levels in the Tartary buckwheat seedlings increased under three types of abiotic stress: drought, salt, and high temperature. A co-expression network of FtMAPK cascade genes was constructed, based on gene expression levels under different light conditions, and co-expressed genes annotated by Gene Ontology and Kyoto Encyclopedia of Genes and Genomes enrichment analyses, which identified numerous transcription factors related to plant abiotic stress. The authors conclude that FtMAPK cascade genes have important roles in the growth and development of Tartary buckwheat, as well as its responses to abiotic stress.
format Online
Article
Text
id pubmed-9313540
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher Frontiers Media S.A.
record_format MEDLINE/PubMed
spelling pubmed-93135402022-07-26 Genome-Wide Identification of MAPK, MAPKK, and MAPKKK Gene Families in Fagopyrum tataricum and Analysis of Their Expression Patterns Under Abiotic Stress Wang, Zhen Yan, Song Ren, Weichao Liu, Yan Sun, Wei Liu, Meiqi Lu, Jiaxin Mi, Yaolei Ma, Wei Front Genet Genetics The mitogen-activated protein kinase (MAPK) cascade is a highly conserved signal transduction pathway, ubiquitous in eukaryotes, such as animals and plants. The MAPK cascade has a dominant role in regulating plant adaptation to the environment, such as through stress responses, osmotic adjustment, and processes that modulate pathogenicity. In the present study, the MAPK cascade gene family was identified in Fagopyrum tataricum (Tartary buckwheat), based on complete genome sequence data. Using phylogenetic tree, conservative motif, and chromosome location analyses, a total of 65 FtMAPK cascade genes, distributed on five chromosomes, were classified into three families: MAPK (n = 8), MAPKK (n = 1), and MAPKKK (n = 56). Transcriptome data from Tartary buckwheat seedlings grown under different light conditions demonstrated that, under blue and red light, the expression levels of 18 and 36 FtMAPK cascade genes were up-regulated and down-regulated, respectively. Through qRT-PCR experiments, it was observed that FtMAPK5, FtMAPKK1, FtMAPKKK8, FtMAPKKK10, and FtMAPKKK24 gene expression levels in the Tartary buckwheat seedlings increased under three types of abiotic stress: drought, salt, and high temperature. A co-expression network of FtMAPK cascade genes was constructed, based on gene expression levels under different light conditions, and co-expressed genes annotated by Gene Ontology and Kyoto Encyclopedia of Genes and Genomes enrichment analyses, which identified numerous transcription factors related to plant abiotic stress. The authors conclude that FtMAPK cascade genes have important roles in the growth and development of Tartary buckwheat, as well as its responses to abiotic stress. Frontiers Media S.A. 2022-06-17 /pmc/articles/PMC9313540/ /pubmed/35899198 http://dx.doi.org/10.3389/fgene.2022.894048 Text en Copyright © 2022 Wang, Yan, Ren, Liu, Sun, Liu, Lu, Mi and Ma. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Genetics
Wang, Zhen
Yan, Song
Ren, Weichao
Liu, Yan
Sun, Wei
Liu, Meiqi
Lu, Jiaxin
Mi, Yaolei
Ma, Wei
Genome-Wide Identification of MAPK, MAPKK, and MAPKKK Gene Families in Fagopyrum tataricum and Analysis of Their Expression Patterns Under Abiotic Stress
title Genome-Wide Identification of MAPK, MAPKK, and MAPKKK Gene Families in Fagopyrum tataricum and Analysis of Their Expression Patterns Under Abiotic Stress
title_full Genome-Wide Identification of MAPK, MAPKK, and MAPKKK Gene Families in Fagopyrum tataricum and Analysis of Their Expression Patterns Under Abiotic Stress
title_fullStr Genome-Wide Identification of MAPK, MAPKK, and MAPKKK Gene Families in Fagopyrum tataricum and Analysis of Their Expression Patterns Under Abiotic Stress
title_full_unstemmed Genome-Wide Identification of MAPK, MAPKK, and MAPKKK Gene Families in Fagopyrum tataricum and Analysis of Their Expression Patterns Under Abiotic Stress
title_short Genome-Wide Identification of MAPK, MAPKK, and MAPKKK Gene Families in Fagopyrum tataricum and Analysis of Their Expression Patterns Under Abiotic Stress
title_sort genome-wide identification of mapk, mapkk, and mapkkk gene families in fagopyrum tataricum and analysis of their expression patterns under abiotic stress
topic Genetics
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9313540/
https://www.ncbi.nlm.nih.gov/pubmed/35899198
http://dx.doi.org/10.3389/fgene.2022.894048
work_keys_str_mv AT wangzhen genomewideidentificationofmapkmapkkandmapkkkgenefamiliesinfagopyrumtataricumandanalysisoftheirexpressionpatternsunderabioticstress
AT yansong genomewideidentificationofmapkmapkkandmapkkkgenefamiliesinfagopyrumtataricumandanalysisoftheirexpressionpatternsunderabioticstress
AT renweichao genomewideidentificationofmapkmapkkandmapkkkgenefamiliesinfagopyrumtataricumandanalysisoftheirexpressionpatternsunderabioticstress
AT liuyan genomewideidentificationofmapkmapkkandmapkkkgenefamiliesinfagopyrumtataricumandanalysisoftheirexpressionpatternsunderabioticstress
AT sunwei genomewideidentificationofmapkmapkkandmapkkkgenefamiliesinfagopyrumtataricumandanalysisoftheirexpressionpatternsunderabioticstress
AT liumeiqi genomewideidentificationofmapkmapkkandmapkkkgenefamiliesinfagopyrumtataricumandanalysisoftheirexpressionpatternsunderabioticstress
AT lujiaxin genomewideidentificationofmapkmapkkandmapkkkgenefamiliesinfagopyrumtataricumandanalysisoftheirexpressionpatternsunderabioticstress
AT miyaolei genomewideidentificationofmapkmapkkandmapkkkgenefamiliesinfagopyrumtataricumandanalysisoftheirexpressionpatternsunderabioticstress
AT mawei genomewideidentificationofmapkmapkkandmapkkkgenefamiliesinfagopyrumtataricumandanalysisoftheirexpressionpatternsunderabioticstress