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Integrated metagenomics and metabolomics analysis illustrates the systemic impact of the gut microbiota on host metabolism after bariatric surgery

AIM: To explore how bariatric surgery (BS) modified the obesity‐associated gut microbiome, the host metabolome, and their interactions in obese Korean patients. MATERIALS AND METHODS: Stool and fasting blood samples were obtained before and 1, 3, 6, and 12 months after BS from 52 patients enrolled i...

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Autores principales: Han, Yeyoung, Kim, Gihyeon, Ahn, Eunyong, Jung, Sunhee, Jung, Youngae, Kim, Yunjae, Ha, Eunyoung, Heo, Yoonseok, Ryu, Do Hyun, Park, Hansoo, Hwang, Geum‐Sook
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Blackwell Publishing Ltd 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9313881/
https://www.ncbi.nlm.nih.gov/pubmed/35257467
http://dx.doi.org/10.1111/dom.14689
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author Han, Yeyoung
Kim, Gihyeon
Ahn, Eunyong
Jung, Sunhee
Jung, Youngae
Kim, Yunjae
Ha, Eunyoung
Heo, Yoonseok
Ryu, Do Hyun
Park, Hansoo
Hwang, Geum‐Sook
author_facet Han, Yeyoung
Kim, Gihyeon
Ahn, Eunyong
Jung, Sunhee
Jung, Youngae
Kim, Yunjae
Ha, Eunyoung
Heo, Yoonseok
Ryu, Do Hyun
Park, Hansoo
Hwang, Geum‐Sook
author_sort Han, Yeyoung
collection PubMed
description AIM: To explore how bariatric surgery (BS) modified the obesity‐associated gut microbiome, the host metabolome, and their interactions in obese Korean patients. MATERIALS AND METHODS: Stool and fasting blood samples were obtained before and 1, 3, 6, and 12 months after BS from 52 patients enrolled in the Korean Obesity Surgical Treatment Study. We analysed the gut microbiome by 16S rRNA gene sequencing and the serum metabolome, including bile acids, by nuclear magnetic resonance spectroscopy and ultrahigh‐performance liquid chromatography/triple quadrupole mass spectrometry. RESULTS: Stool metagenomics showed that 27 microbiota were enriched and 14 microbiota were reduced after BS, whereas the abundances and diversity of observed features were increased. The levels of branched‐chain amino acids and metabolites of energy metabolism in serum were decreased after surgery, whereas the levels of metabolites related to microbial metabolism, including dimethyl sulphone, glycine, and secondary bile acids, were increased in the serum samples. In addition, we found notable mutual associations among metabolites and gut microbiome changes attributed to BS. CONCLUSIONS: Changes in the gut microbiome community and systemic levels of amino acids and sugars were directly derived from anatomical changes in the gastrointestinal tract after BS. We hypothesized that the observed increases in microbiome‐related serum metabolites were a result of complex and indirect changes derived from BS. Ethnic‐specific environmental or genetic factors could affect Korean‐specific postmetabolic modification in obese patients who undergo BS.
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spelling pubmed-93138812022-07-30 Integrated metagenomics and metabolomics analysis illustrates the systemic impact of the gut microbiota on host metabolism after bariatric surgery Han, Yeyoung Kim, Gihyeon Ahn, Eunyong Jung, Sunhee Jung, Youngae Kim, Yunjae Ha, Eunyoung Heo, Yoonseok Ryu, Do Hyun Park, Hansoo Hwang, Geum‐Sook Diabetes Obes Metab Original Articles AIM: To explore how bariatric surgery (BS) modified the obesity‐associated gut microbiome, the host metabolome, and their interactions in obese Korean patients. MATERIALS AND METHODS: Stool and fasting blood samples were obtained before and 1, 3, 6, and 12 months after BS from 52 patients enrolled in the Korean Obesity Surgical Treatment Study. We analysed the gut microbiome by 16S rRNA gene sequencing and the serum metabolome, including bile acids, by nuclear magnetic resonance spectroscopy and ultrahigh‐performance liquid chromatography/triple quadrupole mass spectrometry. RESULTS: Stool metagenomics showed that 27 microbiota were enriched and 14 microbiota were reduced after BS, whereas the abundances and diversity of observed features were increased. The levels of branched‐chain amino acids and metabolites of energy metabolism in serum were decreased after surgery, whereas the levels of metabolites related to microbial metabolism, including dimethyl sulphone, glycine, and secondary bile acids, were increased in the serum samples. In addition, we found notable mutual associations among metabolites and gut microbiome changes attributed to BS. CONCLUSIONS: Changes in the gut microbiome community and systemic levels of amino acids and sugars were directly derived from anatomical changes in the gastrointestinal tract after BS. We hypothesized that the observed increases in microbiome‐related serum metabolites were a result of complex and indirect changes derived from BS. Ethnic‐specific environmental or genetic factors could affect Korean‐specific postmetabolic modification in obese patients who undergo BS. Blackwell Publishing Ltd 2022-03-24 2022-07 /pmc/articles/PMC9313881/ /pubmed/35257467 http://dx.doi.org/10.1111/dom.14689 Text en © 2022 The Authors. Diabetes, Obesity and Metabolism published by John Wiley & Sons Ltd. https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc-nd/4.0/ (https://creativecommons.org/licenses/by-nc-nd/4.0/) License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made.
spellingShingle Original Articles
Han, Yeyoung
Kim, Gihyeon
Ahn, Eunyong
Jung, Sunhee
Jung, Youngae
Kim, Yunjae
Ha, Eunyoung
Heo, Yoonseok
Ryu, Do Hyun
Park, Hansoo
Hwang, Geum‐Sook
Integrated metagenomics and metabolomics analysis illustrates the systemic impact of the gut microbiota on host metabolism after bariatric surgery
title Integrated metagenomics and metabolomics analysis illustrates the systemic impact of the gut microbiota on host metabolism after bariatric surgery
title_full Integrated metagenomics and metabolomics analysis illustrates the systemic impact of the gut microbiota on host metabolism after bariatric surgery
title_fullStr Integrated metagenomics and metabolomics analysis illustrates the systemic impact of the gut microbiota on host metabolism after bariatric surgery
title_full_unstemmed Integrated metagenomics and metabolomics analysis illustrates the systemic impact of the gut microbiota on host metabolism after bariatric surgery
title_short Integrated metagenomics and metabolomics analysis illustrates the systemic impact of the gut microbiota on host metabolism after bariatric surgery
title_sort integrated metagenomics and metabolomics analysis illustrates the systemic impact of the gut microbiota on host metabolism after bariatric surgery
topic Original Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9313881/
https://www.ncbi.nlm.nih.gov/pubmed/35257467
http://dx.doi.org/10.1111/dom.14689
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