Cargando…
Objective Interpretation of the Rapid Urease Test for Helicobacter pylori Infection Using Colorimetry
BACKGROUND: The rapid urease test (RUT) is a major diagnostic tool for detecting Helicobacter pylori infection. This study aimed to establish an objective method for measuring the color changes in the RUT kit to improve the test’s diagnostic accuracy. METHODS: A UV-visible spectrophotometer was sele...
Autores principales: | , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
The Korean Academy of Medical Sciences
2022
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9313973/ https://www.ncbi.nlm.nih.gov/pubmed/35880503 http://dx.doi.org/10.3346/jkms.2022.37.e227 |
_version_ | 1784754206143414272 |
---|---|
author | Pih, Gyu Young Noh, Jin Hee Ahn, Ji Yong Han, Gill Sang Jung, Hyun Suk Jung, Hwoon-Yong Kim, Jung Mogg |
author_facet | Pih, Gyu Young Noh, Jin Hee Ahn, Ji Yong Han, Gill Sang Jung, Hyun Suk Jung, Hwoon-Yong Kim, Jung Mogg |
author_sort | Pih, Gyu Young |
collection | PubMed |
description | BACKGROUND: The rapid urease test (RUT) is a major diagnostic tool for detecting Helicobacter pylori infection. This study aimed to establish an objective method for measuring the color changes in the RUT kit to improve the test’s diagnostic accuracy. METHODS: A UV-visible spectrophotometer was selected as the colorimeter; experiments were conducted in three stages to objectively identify the color changes in the RUT kit. RESULTS: First, the urea broth solution showed an identifiable color change from yellow to red as the pH increased by 0.2. The largest transmittance difference detected using the UV-visible spectrophotometer was observed at a 590-nm wavelength. Second, the commercialized RUT kit also showed a gradual color change according to the pH change detected using the UV-visible spectrophotometer. Third, 13 cases of negative RUT results with a biopsy specimen and 16 of positive RUT results were collected. The transmittance detected using the UV-visible spectrophotometer showed a clear division between the positive and negative RUT groups; the largest difference was observed at a 559-nm wavelength. The lowest transmittance in the negative RUT group was 64, while the highest in the positive RUT group was 56, at the 559-nm wavelength. The UV-visible spectrophotometry reading showed a consistency of 92.7% compared with that of manual reading. CONCLUSION: A transmittance of 60 at a 559-nm wavelength detected using UV-visible spectrophotometer can be used as a cutoff value for interpreting RUT results; this will help develop an automatic RUT kit reader with a high accuracy. |
format | Online Article Text |
id | pubmed-9313973 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | The Korean Academy of Medical Sciences |
record_format | MEDLINE/PubMed |
spelling | pubmed-93139732022-07-29 Objective Interpretation of the Rapid Urease Test for Helicobacter pylori Infection Using Colorimetry Pih, Gyu Young Noh, Jin Hee Ahn, Ji Yong Han, Gill Sang Jung, Hyun Suk Jung, Hwoon-Yong Kim, Jung Mogg J Korean Med Sci Original Article BACKGROUND: The rapid urease test (RUT) is a major diagnostic tool for detecting Helicobacter pylori infection. This study aimed to establish an objective method for measuring the color changes in the RUT kit to improve the test’s diagnostic accuracy. METHODS: A UV-visible spectrophotometer was selected as the colorimeter; experiments were conducted in three stages to objectively identify the color changes in the RUT kit. RESULTS: First, the urea broth solution showed an identifiable color change from yellow to red as the pH increased by 0.2. The largest transmittance difference detected using the UV-visible spectrophotometer was observed at a 590-nm wavelength. Second, the commercialized RUT kit also showed a gradual color change according to the pH change detected using the UV-visible spectrophotometer. Third, 13 cases of negative RUT results with a biopsy specimen and 16 of positive RUT results were collected. The transmittance detected using the UV-visible spectrophotometer showed a clear division between the positive and negative RUT groups; the largest difference was observed at a 559-nm wavelength. The lowest transmittance in the negative RUT group was 64, while the highest in the positive RUT group was 56, at the 559-nm wavelength. The UV-visible spectrophotometry reading showed a consistency of 92.7% compared with that of manual reading. CONCLUSION: A transmittance of 60 at a 559-nm wavelength detected using UV-visible spectrophotometer can be used as a cutoff value for interpreting RUT results; this will help develop an automatic RUT kit reader with a high accuracy. The Korean Academy of Medical Sciences 2022-07-15 /pmc/articles/PMC9313973/ /pubmed/35880503 http://dx.doi.org/10.3346/jkms.2022.37.e227 Text en © 2022 The Korean Academy of Medical Sciences. https://creativecommons.org/licenses/by-nc/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (https://creativecommons.org/licenses/by-nc/4.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Original Article Pih, Gyu Young Noh, Jin Hee Ahn, Ji Yong Han, Gill Sang Jung, Hyun Suk Jung, Hwoon-Yong Kim, Jung Mogg Objective Interpretation of the Rapid Urease Test for Helicobacter pylori Infection Using Colorimetry |
title | Objective Interpretation of the Rapid Urease Test for Helicobacter pylori Infection Using Colorimetry |
title_full | Objective Interpretation of the Rapid Urease Test for Helicobacter pylori Infection Using Colorimetry |
title_fullStr | Objective Interpretation of the Rapid Urease Test for Helicobacter pylori Infection Using Colorimetry |
title_full_unstemmed | Objective Interpretation of the Rapid Urease Test for Helicobacter pylori Infection Using Colorimetry |
title_short | Objective Interpretation of the Rapid Urease Test for Helicobacter pylori Infection Using Colorimetry |
title_sort | objective interpretation of the rapid urease test for helicobacter pylori infection using colorimetry |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9313973/ https://www.ncbi.nlm.nih.gov/pubmed/35880503 http://dx.doi.org/10.3346/jkms.2022.37.e227 |
work_keys_str_mv | AT pihgyuyoung objectiveinterpretationoftherapidureasetestforhelicobacterpyloriinfectionusingcolorimetry AT nohjinhee objectiveinterpretationoftherapidureasetestforhelicobacterpyloriinfectionusingcolorimetry AT ahnjiyong objectiveinterpretationoftherapidureasetestforhelicobacterpyloriinfectionusingcolorimetry AT hangillsang objectiveinterpretationoftherapidureasetestforhelicobacterpyloriinfectionusingcolorimetry AT junghyunsuk objectiveinterpretationoftherapidureasetestforhelicobacterpyloriinfectionusingcolorimetry AT junghwoonyong objectiveinterpretationoftherapidureasetestforhelicobacterpyloriinfectionusingcolorimetry AT kimjungmogg objectiveinterpretationoftherapidureasetestforhelicobacterpyloriinfectionusingcolorimetry |