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Bioprinting small‐diameter vascular vessel with endothelium and smooth muscle by the approach of two‐step crosslinking process
Three‐dimensional bioprinting shows great potential for autologous vascular grafts due to its simplicity, accuracy, and flexibility. The 6‐mm‐diameter vascular grafts are used in clinic. However, producing small‐diameter vascular grafts are still an enormous challenge. Normally, sacrificial hydrogel...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
John Wiley and Sons Inc.
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9314886/ https://www.ncbi.nlm.nih.gov/pubmed/35244205 http://dx.doi.org/10.1002/bit.28075 |
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author | Jin, Qianheng Jin, Guangzhe Ju, Jihui Xu, Lei Tang, Linfeng Fu, Yi Hou, Ruixing Atala, Anthony Zhao, Weixin |
author_facet | Jin, Qianheng Jin, Guangzhe Ju, Jihui Xu, Lei Tang, Linfeng Fu, Yi Hou, Ruixing Atala, Anthony Zhao, Weixin |
author_sort | Jin, Qianheng |
collection | PubMed |
description | Three‐dimensional bioprinting shows great potential for autologous vascular grafts due to its simplicity, accuracy, and flexibility. The 6‐mm‐diameter vascular grafts are used in clinic. However, producing small‐diameter vascular grafts are still an enormous challenge. Normally, sacrificial hydrogels are used as temporary lumen support to mold tubular structure which will affect the stability of the fabricated structure. In this study, we have developed a new bioprinting approach to fabricating small‐diameter vessel using two‐step crosslinking process. The ¼ lumen wall of bioprinted gelatin mechacrylate (GelMA) flat structure was exposed to ultraviolet (UV) light briefly for gaining certain strength, while ¾ lumen wall showed as concave structure which remained uncrosslinked. Precrosslinked flat structure was merged towards the uncrosslinked concave structure. Two individual structures were combined tightly into an intact tubular structure after receiving more UV exposure time. Complicated tubular structures were constructed by these method. Notably, the GelMA‐based bioink loaded with smooth muscle cells are bioprinted to form the outer layer of the tubular structure and human umbilical vein endothelial cells were seeded onto the inner surface of the tubular structure. A bionic vascular vessel with dual layers was fabricated successfully, and kept good viability and functionality. This study may provide a novel idea for fabricating biomimetic vascular network or other more complicated organs. |
format | Online Article Text |
id | pubmed-9314886 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | John Wiley and Sons Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-93148862022-07-30 Bioprinting small‐diameter vascular vessel with endothelium and smooth muscle by the approach of two‐step crosslinking process Jin, Qianheng Jin, Guangzhe Ju, Jihui Xu, Lei Tang, Linfeng Fu, Yi Hou, Ruixing Atala, Anthony Zhao, Weixin Biotechnol Bioeng ARTICLES Three‐dimensional bioprinting shows great potential for autologous vascular grafts due to its simplicity, accuracy, and flexibility. The 6‐mm‐diameter vascular grafts are used in clinic. However, producing small‐diameter vascular grafts are still an enormous challenge. Normally, sacrificial hydrogels are used as temporary lumen support to mold tubular structure which will affect the stability of the fabricated structure. In this study, we have developed a new bioprinting approach to fabricating small‐diameter vessel using two‐step crosslinking process. The ¼ lumen wall of bioprinted gelatin mechacrylate (GelMA) flat structure was exposed to ultraviolet (UV) light briefly for gaining certain strength, while ¾ lumen wall showed as concave structure which remained uncrosslinked. Precrosslinked flat structure was merged towards the uncrosslinked concave structure. Two individual structures were combined tightly into an intact tubular structure after receiving more UV exposure time. Complicated tubular structures were constructed by these method. Notably, the GelMA‐based bioink loaded with smooth muscle cells are bioprinted to form the outer layer of the tubular structure and human umbilical vein endothelial cells were seeded onto the inner surface of the tubular structure. A bionic vascular vessel with dual layers was fabricated successfully, and kept good viability and functionality. This study may provide a novel idea for fabricating biomimetic vascular network or other more complicated organs. John Wiley and Sons Inc. 2022-03-21 2022-06 /pmc/articles/PMC9314886/ /pubmed/35244205 http://dx.doi.org/10.1002/bit.28075 Text en © 2022 The Authors. Biotechnology and Bioengineering published by Wiley Periodicals LLC. https://creativecommons.org/licenses/by-nc/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc/4.0/ (https://creativecommons.org/licenses/by-nc/4.0/) License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited and is not used for commercial purposes. |
spellingShingle | ARTICLES Jin, Qianheng Jin, Guangzhe Ju, Jihui Xu, Lei Tang, Linfeng Fu, Yi Hou, Ruixing Atala, Anthony Zhao, Weixin Bioprinting small‐diameter vascular vessel with endothelium and smooth muscle by the approach of two‐step crosslinking process |
title | Bioprinting small‐diameter vascular vessel with endothelium and smooth muscle by the approach of two‐step crosslinking process |
title_full | Bioprinting small‐diameter vascular vessel with endothelium and smooth muscle by the approach of two‐step crosslinking process |
title_fullStr | Bioprinting small‐diameter vascular vessel with endothelium and smooth muscle by the approach of two‐step crosslinking process |
title_full_unstemmed | Bioprinting small‐diameter vascular vessel with endothelium and smooth muscle by the approach of two‐step crosslinking process |
title_short | Bioprinting small‐diameter vascular vessel with endothelium and smooth muscle by the approach of two‐step crosslinking process |
title_sort | bioprinting small‐diameter vascular vessel with endothelium and smooth muscle by the approach of two‐step crosslinking process |
topic | ARTICLES |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9314886/ https://www.ncbi.nlm.nih.gov/pubmed/35244205 http://dx.doi.org/10.1002/bit.28075 |
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