Cargando…
SnRK2 subfamily I protein kinases regulate ethylene biosynthesis by phosphorylating HB transcription factors to induce ACO1 expression in apple
Ethylene (ETH) controls climacteric fruit ripening and can be triggered by osmotic stress. However, the mechanism regulating ETH biosynthesis during fruit ripening and under osmotic stress is largely unknown in apple (Malus domestica). Here, we explored the roles of SnRK2 protein kinases in ETH bios...
Autores principales: | , , , , , , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
John Wiley and Sons Inc.
2022
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9314909/ https://www.ncbi.nlm.nih.gov/pubmed/35182082 http://dx.doi.org/10.1111/nph.18040 |
_version_ | 1784754432045481984 |
---|---|
author | Jia, Meiru Li, Xingliang Wang, Wei Li, Tianyu Dai, Zhengrong Chen, Yating Zhang, Kaikai Zhu, Haocheng Mao, Wenwen Feng, Qianqian Liu, Liping Yan, Jiaqi Zhong, Silin Li, Bingbing Jia, Wensuo |
author_facet | Jia, Meiru Li, Xingliang Wang, Wei Li, Tianyu Dai, Zhengrong Chen, Yating Zhang, Kaikai Zhu, Haocheng Mao, Wenwen Feng, Qianqian Liu, Liping Yan, Jiaqi Zhong, Silin Li, Bingbing Jia, Wensuo |
author_sort | Jia, Meiru |
collection | PubMed |
description | Ethylene (ETH) controls climacteric fruit ripening and can be triggered by osmotic stress. However, the mechanism regulating ETH biosynthesis during fruit ripening and under osmotic stress is largely unknown in apple (Malus domestica). Here, we explored the roles of SnRK2 protein kinases in ETH biosynthesis related to fruit ripening and osmoregulation. We identified the substrates of MdSnRK2‐I using phosphorylation analysis techniques. Finally, we identified the MdSnRK2‐I‐mediated signaling pathway for ETH biosynthesis related to fruit ripening and osmoregulation. The activity of two MdSnRK2‐I members, MdSnRK2.4 and MdSnRK2.9, was significantly upregulated during ripening or following mannitol treatment. Overexpression of MdSnRK2‐I increased ETH biosynthesis under normal and osmotic conditions in apple fruit. MdSnRK2‐I phosphorylated the transcription factors MdHB1 and MdHB2 to enhance their protein stability and transcriptional activity on MdACO1. MdSnRK2‐I also interacted with MdACS1 and increased its protein stability through two phosphorylation sites. The increased MdACO1 expression and MdACS1 protein stability resulted in higher ETH production in apple fruit. In addition, heterologous expression of MdSnRK2‐I or manipulation of SlSnRK2‐I expression in tomato (Solanum lycopersicum) fruit altered fruit ripening and ETH biosynthesis. We established that MdSnRK2‐I functions in fruit ripening and osmoregulation, and identified the MdSnRK2‐I‐mediated signaling pathway controlling ETH biosynthesis. |
format | Online Article Text |
id | pubmed-9314909 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | John Wiley and Sons Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-93149092022-07-30 SnRK2 subfamily I protein kinases regulate ethylene biosynthesis by phosphorylating HB transcription factors to induce ACO1 expression in apple Jia, Meiru Li, Xingliang Wang, Wei Li, Tianyu Dai, Zhengrong Chen, Yating Zhang, Kaikai Zhu, Haocheng Mao, Wenwen Feng, Qianqian Liu, Liping Yan, Jiaqi Zhong, Silin Li, Bingbing Jia, Wensuo New Phytol Research Ethylene (ETH) controls climacteric fruit ripening and can be triggered by osmotic stress. However, the mechanism regulating ETH biosynthesis during fruit ripening and under osmotic stress is largely unknown in apple (Malus domestica). Here, we explored the roles of SnRK2 protein kinases in ETH biosynthesis related to fruit ripening and osmoregulation. We identified the substrates of MdSnRK2‐I using phosphorylation analysis techniques. Finally, we identified the MdSnRK2‐I‐mediated signaling pathway for ETH biosynthesis related to fruit ripening and osmoregulation. The activity of two MdSnRK2‐I members, MdSnRK2.4 and MdSnRK2.9, was significantly upregulated during ripening or following mannitol treatment. Overexpression of MdSnRK2‐I increased ETH biosynthesis under normal and osmotic conditions in apple fruit. MdSnRK2‐I phosphorylated the transcription factors MdHB1 and MdHB2 to enhance their protein stability and transcriptional activity on MdACO1. MdSnRK2‐I also interacted with MdACS1 and increased its protein stability through two phosphorylation sites. The increased MdACO1 expression and MdACS1 protein stability resulted in higher ETH production in apple fruit. In addition, heterologous expression of MdSnRK2‐I or manipulation of SlSnRK2‐I expression in tomato (Solanum lycopersicum) fruit altered fruit ripening and ETH biosynthesis. We established that MdSnRK2‐I functions in fruit ripening and osmoregulation, and identified the MdSnRK2‐I‐mediated signaling pathway controlling ETH biosynthesis. John Wiley and Sons Inc. 2022-03-22 2022-05 /pmc/articles/PMC9314909/ /pubmed/35182082 http://dx.doi.org/10.1111/nph.18040 Text en © 2022 The Authors. New Phytologist © 2022 New Phytologist Foundation https://creativecommons.org/licenses/by/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Jia, Meiru Li, Xingliang Wang, Wei Li, Tianyu Dai, Zhengrong Chen, Yating Zhang, Kaikai Zhu, Haocheng Mao, Wenwen Feng, Qianqian Liu, Liping Yan, Jiaqi Zhong, Silin Li, Bingbing Jia, Wensuo SnRK2 subfamily I protein kinases regulate ethylene biosynthesis by phosphorylating HB transcription factors to induce ACO1 expression in apple |
title | SnRK2 subfamily I protein kinases regulate ethylene biosynthesis by phosphorylating HB transcription factors to induce ACO1 expression in apple |
title_full | SnRK2 subfamily I protein kinases regulate ethylene biosynthesis by phosphorylating HB transcription factors to induce ACO1 expression in apple |
title_fullStr | SnRK2 subfamily I protein kinases regulate ethylene biosynthesis by phosphorylating HB transcription factors to induce ACO1 expression in apple |
title_full_unstemmed | SnRK2 subfamily I protein kinases regulate ethylene biosynthesis by phosphorylating HB transcription factors to induce ACO1 expression in apple |
title_short | SnRK2 subfamily I protein kinases regulate ethylene biosynthesis by phosphorylating HB transcription factors to induce ACO1 expression in apple |
title_sort | snrk2 subfamily i protein kinases regulate ethylene biosynthesis by phosphorylating hb transcription factors to induce aco1 expression in apple |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9314909/ https://www.ncbi.nlm.nih.gov/pubmed/35182082 http://dx.doi.org/10.1111/nph.18040 |
work_keys_str_mv | AT jiameiru snrk2subfamilyiproteinkinasesregulateethylenebiosynthesisbyphosphorylatinghbtranscriptionfactorstoinduceaco1expressioninapple AT lixingliang snrk2subfamilyiproteinkinasesregulateethylenebiosynthesisbyphosphorylatinghbtranscriptionfactorstoinduceaco1expressioninapple AT wangwei snrk2subfamilyiproteinkinasesregulateethylenebiosynthesisbyphosphorylatinghbtranscriptionfactorstoinduceaco1expressioninapple AT litianyu snrk2subfamilyiproteinkinasesregulateethylenebiosynthesisbyphosphorylatinghbtranscriptionfactorstoinduceaco1expressioninapple AT daizhengrong snrk2subfamilyiproteinkinasesregulateethylenebiosynthesisbyphosphorylatinghbtranscriptionfactorstoinduceaco1expressioninapple AT chenyating snrk2subfamilyiproteinkinasesregulateethylenebiosynthesisbyphosphorylatinghbtranscriptionfactorstoinduceaco1expressioninapple AT zhangkaikai snrk2subfamilyiproteinkinasesregulateethylenebiosynthesisbyphosphorylatinghbtranscriptionfactorstoinduceaco1expressioninapple AT zhuhaocheng snrk2subfamilyiproteinkinasesregulateethylenebiosynthesisbyphosphorylatinghbtranscriptionfactorstoinduceaco1expressioninapple AT maowenwen snrk2subfamilyiproteinkinasesregulateethylenebiosynthesisbyphosphorylatinghbtranscriptionfactorstoinduceaco1expressioninapple AT fengqianqian snrk2subfamilyiproteinkinasesregulateethylenebiosynthesisbyphosphorylatinghbtranscriptionfactorstoinduceaco1expressioninapple AT liuliping snrk2subfamilyiproteinkinasesregulateethylenebiosynthesisbyphosphorylatinghbtranscriptionfactorstoinduceaco1expressioninapple AT yanjiaqi snrk2subfamilyiproteinkinasesregulateethylenebiosynthesisbyphosphorylatinghbtranscriptionfactorstoinduceaco1expressioninapple AT zhongsilin snrk2subfamilyiproteinkinasesregulateethylenebiosynthesisbyphosphorylatinghbtranscriptionfactorstoinduceaco1expressioninapple AT libingbing snrk2subfamilyiproteinkinasesregulateethylenebiosynthesisbyphosphorylatinghbtranscriptionfactorstoinduceaco1expressioninapple AT jiawensuo snrk2subfamilyiproteinkinasesregulateethylenebiosynthesisbyphosphorylatinghbtranscriptionfactorstoinduceaco1expressioninapple |