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Rapid and efficient generation of antigen‐specific isogenic T cells from cryopreserved blood samples
Clustered regularly interspaced short palindromic repeats/CRISPR‐associated protein 9 (CRISPR/Cas9)‐mediated gene editing has been leveraged for the modification of human and mouse T cells. However, limited experience is available on the application of CRISPR/Cas9 electroporation in cryopreserved T...
Autores principales: | Eerkens, Anneke L, Vledder, Annegé, van Rooij, Nienke, Foijer, Floris, Nijman, Hans W, de Bruyn, Marco |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
John Wiley and Sons Inc.
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9314923/ https://www.ncbi.nlm.nih.gov/pubmed/35194830 http://dx.doi.org/10.1111/imcb.12538 |
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