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Sodium Selenite Promotes Osteoblast Differentiation via The WNT/ß-Catenin Signaling Pathway

OBJECTIVES: Osteoporosis is regarded as a silent disorder affecting bone slowly, leading to an increased risk of fractures. Lately, selenium has been found to be associated with the acquisition and maintenance of bone health by affecting the bone remodeling process. However, the mechanism of action...

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Autores principales: Sharma, Ashish Ranjan, Sharma, Garima, Lee, Yeon-Hee, Chakraborty, Chiranjib, Lee, Sang-Soo, Seo, Eun-Min
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Royan Institute 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9315210/
https://www.ncbi.nlm.nih.gov/pubmed/35892229
http://dx.doi.org/10.22074/cellj.2022.8314
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author Sharma, Ashish Ranjan
Sharma, Garima
Lee, Yeon-Hee
Chakraborty, Chiranjib
Lee, Sang-Soo
Seo, Eun-Min
author_facet Sharma, Ashish Ranjan
Sharma, Garima
Lee, Yeon-Hee
Chakraborty, Chiranjib
Lee, Sang-Soo
Seo, Eun-Min
author_sort Sharma, Ashish Ranjan
collection PubMed
description OBJECTIVES: Osteoporosis is regarded as a silent disorder affecting bone slowly, leading to an increased risk of fractures. Lately, selenium has been found to be associated with the acquisition and maintenance of bone health by affecting the bone remodeling process. However, the mechanism of action of selenium on bone is poorly understood. Here, the objective of this study is to examine the protective effects and mechanism of sodium selenite on the differentiation process of osteoblasts as well as under oxidative stress-induced conditions by evaluating the expression of osteoblast differentiation markers in the sodium selenite and/or hydrogen peroxide (H(2)O(2))-treated MC3T3-E1 cell line. MATERIALS AND METHODS: In this experimental study, we confirmed the inducible osteogenic effect of sodium selenite on MC3T3-E1 cells. Moreover, we investigated the recovery of expression levels of osteogenic markers of sodium selenite in H(2)O(2)-treated MC3T3-E1 cells. RESULTS: It was observed that sodium selenite could promote alkaline phosphatase (ALP) activity and collagen synthesis in pre-osteoblasts. Also, sodium selenite enhanced the mRNA expression levels of osteogenic transcriptional factors, like osterix (OSX) and runt-related transcription factor 2 (Runx2). In addition, the terminal differentiation markers, such as osteocalcin (OCN) and collagen 1α (Col1α) were also increased after the treatment of sodium selenite. Also treatment of sodium selenite recused the H(2)O(2)-induced inhibition of osteoblastic differentiation of pre-osteoblasts cells via the WNT signaling pathway, implicating its antioxidant activity. Furthermore, sodium selenite restored the H2O2 repressed β-catenin stability and axin-2 reporter activity in MC3T3-E1 cells. CONCLUSION: It may be concluded that sodium selenite can stimulate bone formation and rescue the oxidative repression of osteogenesis by activating WNT signaling pathways. Further detailed studies on the role of selenium and its ability to stimulate bone formation via the WNT signaling pathway may project it as a potential therapeutic intervention for osteoporosis.
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spelling pubmed-93152102022-07-28 Sodium Selenite Promotes Osteoblast Differentiation via The WNT/ß-Catenin Signaling Pathway Sharma, Ashish Ranjan Sharma, Garima Lee, Yeon-Hee Chakraborty, Chiranjib Lee, Sang-Soo Seo, Eun-Min Cell J Original Article OBJECTIVES: Osteoporosis is regarded as a silent disorder affecting bone slowly, leading to an increased risk of fractures. Lately, selenium has been found to be associated with the acquisition and maintenance of bone health by affecting the bone remodeling process. However, the mechanism of action of selenium on bone is poorly understood. Here, the objective of this study is to examine the protective effects and mechanism of sodium selenite on the differentiation process of osteoblasts as well as under oxidative stress-induced conditions by evaluating the expression of osteoblast differentiation markers in the sodium selenite and/or hydrogen peroxide (H(2)O(2))-treated MC3T3-E1 cell line. MATERIALS AND METHODS: In this experimental study, we confirmed the inducible osteogenic effect of sodium selenite on MC3T3-E1 cells. Moreover, we investigated the recovery of expression levels of osteogenic markers of sodium selenite in H(2)O(2)-treated MC3T3-E1 cells. RESULTS: It was observed that sodium selenite could promote alkaline phosphatase (ALP) activity and collagen synthesis in pre-osteoblasts. Also, sodium selenite enhanced the mRNA expression levels of osteogenic transcriptional factors, like osterix (OSX) and runt-related transcription factor 2 (Runx2). In addition, the terminal differentiation markers, such as osteocalcin (OCN) and collagen 1α (Col1α) were also increased after the treatment of sodium selenite. Also treatment of sodium selenite recused the H(2)O(2)-induced inhibition of osteoblastic differentiation of pre-osteoblasts cells via the WNT signaling pathway, implicating its antioxidant activity. Furthermore, sodium selenite restored the H2O2 repressed β-catenin stability and axin-2 reporter activity in MC3T3-E1 cells. CONCLUSION: It may be concluded that sodium selenite can stimulate bone formation and rescue the oxidative repression of osteogenesis by activating WNT signaling pathways. Further detailed studies on the role of selenium and its ability to stimulate bone formation via the WNT signaling pathway may project it as a potential therapeutic intervention for osteoporosis. Royan Institute 2022-06 2022-06-29 /pmc/articles/PMC9315210/ /pubmed/35892229 http://dx.doi.org/10.22074/cellj.2022.8314 Text en Any use, distribution, reproduction or abstract of this publication in any medium, with the exception of commercial purposes, is permitted provided the original work is properly cited. https://creativecommons.org/licenses/by-nc/3.0/This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial 3.0 (CC BY-NC 3.0) License, which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Article
Sharma, Ashish Ranjan
Sharma, Garima
Lee, Yeon-Hee
Chakraborty, Chiranjib
Lee, Sang-Soo
Seo, Eun-Min
Sodium Selenite Promotes Osteoblast Differentiation via The WNT/ß-Catenin Signaling Pathway
title Sodium Selenite Promotes Osteoblast Differentiation via The WNT/ß-Catenin Signaling Pathway
title_full Sodium Selenite Promotes Osteoblast Differentiation via The WNT/ß-Catenin Signaling Pathway
title_fullStr Sodium Selenite Promotes Osteoblast Differentiation via The WNT/ß-Catenin Signaling Pathway
title_full_unstemmed Sodium Selenite Promotes Osteoblast Differentiation via The WNT/ß-Catenin Signaling Pathway
title_short Sodium Selenite Promotes Osteoblast Differentiation via The WNT/ß-Catenin Signaling Pathway
title_sort sodium selenite promotes osteoblast differentiation via the wnt/ß-catenin signaling pathway
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9315210/
https://www.ncbi.nlm.nih.gov/pubmed/35892229
http://dx.doi.org/10.22074/cellj.2022.8314
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