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Comparison by Age of the Local Interferon Response to SARS-CoV-2 Suggests a Role for IFN-ε and -ω

Children generally develop a mild disease after SARS-CoV-2 infection whereas older adults are at risk of developing severe COVID-19. Recent transcriptomic analysis showed pre-activated innate immunity in children, resulting in a more effective anti-SARS-CoV-2 response upon infection. To further char...

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Autores principales: Pierangeli, Alessandra, Gentile, Massimo, Oliveto, Giuseppe, Frasca, Federica, Sorrentino, Leonardo, Matera, Luigi, Nenna, Raffaella, Viscido, Agnese, Fracella, Matteo, Petrarca, Laura, D’Ettorre, Gabriella, Ceccarelli, Giancarlo, Midulla, Fabio, Antonelli, Guido, Scagnolari, Carolina
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9315386/
https://www.ncbi.nlm.nih.gov/pubmed/35903094
http://dx.doi.org/10.3389/fimmu.2022.873232
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author Pierangeli, Alessandra
Gentile, Massimo
Oliveto, Giuseppe
Frasca, Federica
Sorrentino, Leonardo
Matera, Luigi
Nenna, Raffaella
Viscido, Agnese
Fracella, Matteo
Petrarca, Laura
D’Ettorre, Gabriella
Ceccarelli, Giancarlo
Midulla, Fabio
Antonelli, Guido
Scagnolari, Carolina
author_facet Pierangeli, Alessandra
Gentile, Massimo
Oliveto, Giuseppe
Frasca, Federica
Sorrentino, Leonardo
Matera, Luigi
Nenna, Raffaella
Viscido, Agnese
Fracella, Matteo
Petrarca, Laura
D’Ettorre, Gabriella
Ceccarelli, Giancarlo
Midulla, Fabio
Antonelli, Guido
Scagnolari, Carolina
author_sort Pierangeli, Alessandra
collection PubMed
description Children generally develop a mild disease after SARS-CoV-2 infection whereas older adults are at risk of developing severe COVID-19. Recent transcriptomic analysis showed pre-activated innate immunity in children, resulting in a more effective anti-SARS-CoV-2 response upon infection. To further characterize age-related differences, we studied type I and III interferon (IFN) response in SARS-CoV-2 infected and non-infected individuals of different ages. Specifically, levels of expression of type I (IFN-α, -β, -ε and -ω), type III (IFN-λ1, -λ2 and -λ3) IFNs and of the IFN-stimulated genes, ISG15 and ISG56 were quantified in nasopharyngeal cells from diagnostic swabs. Basal transcription of type I/III IFN genes was highest among children and decreased with age. Among SARS-CoV-2-infected individuals, only IFN-ε and -ω levels were significantly higher in children and young adults whereas ISGs were overexpressed in infected adults. The occurrence of symptoms in children and the need for hospitalization in adults were associated to higher transcription of several IFN genes. Starting from a pre-activated transcription level, the expression of type I and III IFNs was not highly up-regulated in children upon SARS-CoV-2 infection; young adults activated IFNs’ transcription at intermediate levels whereas older adults were characterized by higher ISGs and lower IFN-ε and -ω relative expression levels. Overall, our findings contribute to recognize components of a protective IFN response as a function of age, in the context of SARS-CoV-2 infection.
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spelling pubmed-93153862022-07-27 Comparison by Age of the Local Interferon Response to SARS-CoV-2 Suggests a Role for IFN-ε and -ω Pierangeli, Alessandra Gentile, Massimo Oliveto, Giuseppe Frasca, Federica Sorrentino, Leonardo Matera, Luigi Nenna, Raffaella Viscido, Agnese Fracella, Matteo Petrarca, Laura D’Ettorre, Gabriella Ceccarelli, Giancarlo Midulla, Fabio Antonelli, Guido Scagnolari, Carolina Front Immunol Immunology Children generally develop a mild disease after SARS-CoV-2 infection whereas older adults are at risk of developing severe COVID-19. Recent transcriptomic analysis showed pre-activated innate immunity in children, resulting in a more effective anti-SARS-CoV-2 response upon infection. To further characterize age-related differences, we studied type I and III interferon (IFN) response in SARS-CoV-2 infected and non-infected individuals of different ages. Specifically, levels of expression of type I (IFN-α, -β, -ε and -ω), type III (IFN-λ1, -λ2 and -λ3) IFNs and of the IFN-stimulated genes, ISG15 and ISG56 were quantified in nasopharyngeal cells from diagnostic swabs. Basal transcription of type I/III IFN genes was highest among children and decreased with age. Among SARS-CoV-2-infected individuals, only IFN-ε and -ω levels were significantly higher in children and young adults whereas ISGs were overexpressed in infected adults. The occurrence of symptoms in children and the need for hospitalization in adults were associated to higher transcription of several IFN genes. Starting from a pre-activated transcription level, the expression of type I and III IFNs was not highly up-regulated in children upon SARS-CoV-2 infection; young adults activated IFNs’ transcription at intermediate levels whereas older adults were characterized by higher ISGs and lower IFN-ε and -ω relative expression levels. Overall, our findings contribute to recognize components of a protective IFN response as a function of age, in the context of SARS-CoV-2 infection. Frontiers Media S.A. 2022-07-12 /pmc/articles/PMC9315386/ /pubmed/35903094 http://dx.doi.org/10.3389/fimmu.2022.873232 Text en Copyright © 2022 Pierangeli, Gentile, Oliveto, Frasca, Sorrentino, Matera, Nenna, Viscido, Fracella, Petrarca, D’Ettorre, Ceccarelli, Midulla, Antonelli and Scagnolari https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Immunology
Pierangeli, Alessandra
Gentile, Massimo
Oliveto, Giuseppe
Frasca, Federica
Sorrentino, Leonardo
Matera, Luigi
Nenna, Raffaella
Viscido, Agnese
Fracella, Matteo
Petrarca, Laura
D’Ettorre, Gabriella
Ceccarelli, Giancarlo
Midulla, Fabio
Antonelli, Guido
Scagnolari, Carolina
Comparison by Age of the Local Interferon Response to SARS-CoV-2 Suggests a Role for IFN-ε and -ω
title Comparison by Age of the Local Interferon Response to SARS-CoV-2 Suggests a Role for IFN-ε and -ω
title_full Comparison by Age of the Local Interferon Response to SARS-CoV-2 Suggests a Role for IFN-ε and -ω
title_fullStr Comparison by Age of the Local Interferon Response to SARS-CoV-2 Suggests a Role for IFN-ε and -ω
title_full_unstemmed Comparison by Age of the Local Interferon Response to SARS-CoV-2 Suggests a Role for IFN-ε and -ω
title_short Comparison by Age of the Local Interferon Response to SARS-CoV-2 Suggests a Role for IFN-ε and -ω
title_sort comparison by age of the local interferon response to sars-cov-2 suggests a role for ifn-ε and -ω
topic Immunology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9315386/
https://www.ncbi.nlm.nih.gov/pubmed/35903094
http://dx.doi.org/10.3389/fimmu.2022.873232
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