An aco-2::gfp knock-in enables the monitoring of mitochondrial morphology throughout C. elegans lifespan.

We used CRISPR/Cas9 gene editing in C. elegans in order to fluorescently tag endogenous aconitase-2 (ACO-2). ACO-2 is a mitochondrially localized protein, and the aco-2::gfp strain enabled the examination of native mitochondrial morphology in live animals. Here we validate that the aco-2::gfp strain...

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Detalles Bibliográficos
Autores principales: Begelman, David V., Woods, Georgia, Bhaumik, Dipa, Angeli, Suzanne, Foulger, Anna C, Lucanic, Mark, Lan, Jianfeng, Andersen, Julie K., Lithgow, Gordon J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Caltech Library 2022
Materias:
Materials and Reagents
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9315405/
https://www.ncbi.nlm.nih.gov/pubmed/35903774
http://dx.doi.org/10.17912/micropub.biology.000599
Descripción
Sumario:We used CRISPR/Cas9 gene editing in C. elegans in order to fluorescently tag endogenous aconitase-2 (ACO-2). ACO-2 is a mitochondrially localized protein, and the aco-2::gfp strain enabled the examination of native mitochondrial morphology in live animals. Here we validate that the aco-2::gfp strain displays the prototypic changes in mitochondrial morphology known to occur during aging and upon paraquat (PQ) induced mitochondrial stress. We also provide evidence that the ACO-2::GFP reporter can serve as a superior means for tracking mitochondrial morphology than conventional MitoTracker dyes—especially in aged-worms.

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