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An aco-2::gfp knock-in enables the monitoring of mitochondrial morphology throughout C. elegans lifespan.

We used CRISPR/Cas9 gene editing in C. elegans in order to fluorescently tag endogenous aconitase-2 (ACO-2). ACO-2 is a mitochondrially localized protein, and the aco-2::gfp strain enabled the examination of native mitochondrial morphology in live animals. Here we validate that the aco-2::gfp strain...

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Detalles Bibliográficos
Autores principales:	Begelman, David V., Woods, Georgia, Bhaumik, Dipa, Angeli, Suzanne, Foulger, Anna C, Lucanic, Mark, Lan, Jianfeng, Andersen, Julie K., Lithgow, Gordon J.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Caltech Library 2022
Materias:	Materials and Reagents
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9315405/ https://www.ncbi.nlm.nih.gov/pubmed/35903774 http://dx.doi.org/10.17912/micropub.biology.000599

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