Single-molecule FISH in C. elegans embryos reveals early embryonic expression dynamics of par-2 , lgl-1 and chin-1 and possible differences between hyper-diverged strains

Wild C. elegans strains harbor natural variation in developmental pathways, but investigating these differences requires precise and well-powered phenotyping methods. Here we employ a microfluidics platform for single-molecule FISH to simultaneously visualize the transcripts of three genes in embryo...

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Autores principales: Kaul, Samiksha, Chou, Han Ting, Charles, Seleipiri, Aubry, Guillaume, Lu, Hang, Paaby, Annalise B.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Caltech Library 2022
Materias:
New Finding
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9315406/
https://www.ncbi.nlm.nih.gov/pubmed/35903776
http://dx.doi.org/10.17912/micropub.biology.000609
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author Kaul, Samiksha
Chou, Han Ting
Charles, Seleipiri
Aubry, Guillaume
Lu, Hang
Paaby, Annalise B.
author_facet Kaul, Samiksha
Chou, Han Ting
Charles, Seleipiri
Aubry, Guillaume
Lu, Hang
Paaby, Annalise B.
author_sort Kaul, Samiksha
collection PubMed
description Wild C. elegans strains harbor natural variation in developmental pathways, but investigating these differences requires precise and well-powered phenotyping methods. Here we employ a microfluidics platform for single-molecule FISH to simultaneously visualize the transcripts of three genes in embryos of two distinct strains. We capture transcripts at high resolution by developmental stage in over one hundred embryos of each strain and observe wide-scale conservation of expression, but subtle differences in par-2 and chin-1 abundance and rate of change. As both genes reside in a genomic interval of hyper-divergence, these results may reflect consequences of pathway evolution over long timescales.
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spelling pubmed-93154062022-07-27 Single-molecule FISH in C. elegans embryos reveals early embryonic expression dynamics of par-2 , lgl-1 and chin-1 and possible differences between hyper-diverged strains Kaul, Samiksha Chou, Han Ting Charles, Seleipiri Aubry, Guillaume Lu, Hang Paaby, Annalise B. MicroPubl Biol New Finding Wild C. elegans strains harbor natural variation in developmental pathways, but investigating these differences requires precise and well-powered phenotyping methods. Here we employ a microfluidics platform for single-molecule FISH to simultaneously visualize the transcripts of three genes in embryos of two distinct strains. We capture transcripts at high resolution by developmental stage in over one hundred embryos of each strain and observe wide-scale conservation of expression, but subtle differences in par-2 and chin-1 abundance and rate of change. As both genes reside in a genomic interval of hyper-divergence, these results may reflect consequences of pathway evolution over long timescales. Caltech Library 2022-07-20 /pmc/articles/PMC9315406/ /pubmed/35903776 http://dx.doi.org/10.17912/micropub.biology.000609 Text en Copyright: © 2022 by the authors https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle New Finding
Kaul, Samiksha
Chou, Han Ting
Charles, Seleipiri
Aubry, Guillaume
Lu, Hang
Paaby, Annalise B.
Single-molecule FISH in C. elegans embryos reveals early embryonic expression dynamics of par-2 , lgl-1 and chin-1 and possible differences between hyper-diverged strains
title Single-molecule FISH in C. elegans embryos reveals early embryonic expression dynamics of par-2 , lgl-1 and chin-1 and possible differences between hyper-diverged strains
title_full Single-molecule FISH in C. elegans embryos reveals early embryonic expression dynamics of par-2 , lgl-1 and chin-1 and possible differences between hyper-diverged strains
title_fullStr Single-molecule FISH in C. elegans embryos reveals early embryonic expression dynamics of par-2 , lgl-1 and chin-1 and possible differences between hyper-diverged strains
title_full_unstemmed Single-molecule FISH in C. elegans embryos reveals early embryonic expression dynamics of par-2 , lgl-1 and chin-1 and possible differences between hyper-diverged strains
title_short Single-molecule FISH in C. elegans embryos reveals early embryonic expression dynamics of par-2 , lgl-1 and chin-1 and possible differences between hyper-diverged strains
title_sort single-molecule fish in c. elegans embryos reveals early embryonic expression dynamics of par-2 , lgl-1 and chin-1 and possible differences between hyper-diverged strains
topic New Finding
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9315406/
https://www.ncbi.nlm.nih.gov/pubmed/35903776
http://dx.doi.org/10.17912/micropub.biology.000609
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