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Single-molecule FISH in C. elegans embryos reveals early embryonic expression dynamics of par-2 , lgl-1 and chin-1 and possible differences between hyper-diverged strains
Wild C. elegans strains harbor natural variation in developmental pathways, but investigating these differences requires precise and well-powered phenotyping methods. Here we employ a microfluidics platform for single-molecule FISH to simultaneously visualize the transcripts of three genes in embryo...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Caltech Library
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9315406/ https://www.ncbi.nlm.nih.gov/pubmed/35903776 http://dx.doi.org/10.17912/micropub.biology.000609 |
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author | Kaul, Samiksha Chou, Han Ting Charles, Seleipiri Aubry, Guillaume Lu, Hang Paaby, Annalise B. |
author_facet | Kaul, Samiksha Chou, Han Ting Charles, Seleipiri Aubry, Guillaume Lu, Hang Paaby, Annalise B. |
author_sort | Kaul, Samiksha |
collection | PubMed |
description | Wild C. elegans strains harbor natural variation in developmental pathways, but investigating these differences requires precise and well-powered phenotyping methods. Here we employ a microfluidics platform for single-molecule FISH to simultaneously visualize the transcripts of three genes in embryos of two distinct strains. We capture transcripts at high resolution by developmental stage in over one hundred embryos of each strain and observe wide-scale conservation of expression, but subtle differences in par-2 and chin-1 abundance and rate of change. As both genes reside in a genomic interval of hyper-divergence, these results may reflect consequences of pathway evolution over long timescales. |
format | Online Article Text |
id | pubmed-9315406 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Caltech Library |
record_format | MEDLINE/PubMed |
spelling | pubmed-93154062022-07-27 Single-molecule FISH in C. elegans embryos reveals early embryonic expression dynamics of par-2 , lgl-1 and chin-1 and possible differences between hyper-diverged strains Kaul, Samiksha Chou, Han Ting Charles, Seleipiri Aubry, Guillaume Lu, Hang Paaby, Annalise B. MicroPubl Biol New Finding Wild C. elegans strains harbor natural variation in developmental pathways, but investigating these differences requires precise and well-powered phenotyping methods. Here we employ a microfluidics platform for single-molecule FISH to simultaneously visualize the transcripts of three genes in embryos of two distinct strains. We capture transcripts at high resolution by developmental stage in over one hundred embryos of each strain and observe wide-scale conservation of expression, but subtle differences in par-2 and chin-1 abundance and rate of change. As both genes reside in a genomic interval of hyper-divergence, these results may reflect consequences of pathway evolution over long timescales. Caltech Library 2022-07-20 /pmc/articles/PMC9315406/ /pubmed/35903776 http://dx.doi.org/10.17912/micropub.biology.000609 Text en Copyright: © 2022 by the authors https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | New Finding Kaul, Samiksha Chou, Han Ting Charles, Seleipiri Aubry, Guillaume Lu, Hang Paaby, Annalise B. Single-molecule FISH in C. elegans embryos reveals early embryonic expression dynamics of par-2 , lgl-1 and chin-1 and possible differences between hyper-diverged strains |
title |
Single-molecule FISH in
C. elegans
embryos reveals early embryonic expression dynamics of
par-2
,
lgl-1
and
chin-1
and possible differences between hyper-diverged strains
|
title_full |
Single-molecule FISH in
C. elegans
embryos reveals early embryonic expression dynamics of
par-2
,
lgl-1
and
chin-1
and possible differences between hyper-diverged strains
|
title_fullStr |
Single-molecule FISH in
C. elegans
embryos reveals early embryonic expression dynamics of
par-2
,
lgl-1
and
chin-1
and possible differences between hyper-diverged strains
|
title_full_unstemmed |
Single-molecule FISH in
C. elegans
embryos reveals early embryonic expression dynamics of
par-2
,
lgl-1
and
chin-1
and possible differences between hyper-diverged strains
|
title_short |
Single-molecule FISH in
C. elegans
embryos reveals early embryonic expression dynamics of
par-2
,
lgl-1
and
chin-1
and possible differences between hyper-diverged strains
|
title_sort | single-molecule fish in
c. elegans
embryos reveals early embryonic expression dynamics of
par-2
,
lgl-1
and
chin-1
and possible differences between hyper-diverged strains |
topic | New Finding |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9315406/ https://www.ncbi.nlm.nih.gov/pubmed/35903776 http://dx.doi.org/10.17912/micropub.biology.000609 |
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