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Exact breakpoints of the In(1)w (m4) rearrangement
In(1)w (m4) has been known for decades as a classic example of a position effect variegation-causing rearrangement and has been mentioned in hundreds of publications. Nevertheless, its euchromatic breakpoint has not been localized with base-pair resolution. We performed nanopore sequencing of DNA fr...
| Autores principales: | , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
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Caltech Library
2022
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9315410/ https://www.ncbi.nlm.nih.gov/pubmed/35903781 http://dx.doi.org/10.17912/micropub.biology.000608 |
| Sumario: | In(1)w (m4) has been known for decades as a classic example of a position effect variegation-causing rearrangement and has been mentioned in hundreds of publications. Nevertheless, its euchromatic breakpoint has not been localized with base-pair resolution. We performed nanopore sequencing of DNA from In(1)w (m4) homozygous flies and determined the exact position of euchromatic (chrX:2767875) and heterochromatic breakpoints of the rearrangement. The heterochromatic breakpoint is located in an unlinked part of the genome in the region, enriched in TEs (transposable elements) fragments. A set of unique piRNAs could be detected in the region. |
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