Exact breakpoints of the In(1)w (m4) rearrangement

In(1)w (m4) has been known for decades as a classic example of a position effect variegation-causing rearrangement and has been mentioned in hundreds of publications. Nevertheless, its euchromatic breakpoint has not been localized with base-pair resolution. We performed nanopore sequencing of DNA fr...

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Detalles Bibliográficos
Autores principales: Solodovnikov, Alexander, Lavrov, Sergey
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Caltech Library 2022
Materias:
Data Updates
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9315410/
https://www.ncbi.nlm.nih.gov/pubmed/35903781
http://dx.doi.org/10.17912/micropub.biology.000608
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author Solodovnikov, Alexander
Lavrov, Sergey
author_facet Solodovnikov, Alexander
Lavrov, Sergey
author_sort Solodovnikov, Alexander
collection PubMed
description In(1)w (m4) has been known for decades as a classic example of a position effect variegation-causing rearrangement and has been mentioned in hundreds of publications. Nevertheless, its euchromatic breakpoint has not been localized with base-pair resolution. We performed nanopore sequencing of DNA from In(1)w (m4) homozygous flies and determined the exact position of euchromatic (chrX:2767875) and heterochromatic breakpoints of the rearrangement. The heterochromatic breakpoint is located in an unlinked part of the genome in the region, enriched in TEs (transposable elements) fragments. A set of unique piRNAs could be detected in the region.
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spelling pubmed-93154102022-07-27 Exact breakpoints of the In(1)w (m4) rearrangement Solodovnikov, Alexander Lavrov, Sergey MicroPubl Biol Data Updates In(1)w (m4) has been known for decades as a classic example of a position effect variegation-causing rearrangement and has been mentioned in hundreds of publications. Nevertheless, its euchromatic breakpoint has not been localized with base-pair resolution. We performed nanopore sequencing of DNA from In(1)w (m4) homozygous flies and determined the exact position of euchromatic (chrX:2767875) and heterochromatic breakpoints of the rearrangement. The heterochromatic breakpoint is located in an unlinked part of the genome in the region, enriched in TEs (transposable elements) fragments. A set of unique piRNAs could be detected in the region. Caltech Library 2022-07-25 /pmc/articles/PMC9315410/ /pubmed/35903781 http://dx.doi.org/10.17912/micropub.biology.000608 Text en Copyright: © 2022 by the authors https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Data Updates
Solodovnikov, Alexander
Lavrov, Sergey
Exact breakpoints of the In(1)w (m4) rearrangement
title Exact breakpoints of the In(1)w (m4) rearrangement
title_full Exact breakpoints of the In(1)w (m4) rearrangement
title_fullStr Exact breakpoints of the In(1)w (m4) rearrangement
title_full_unstemmed Exact breakpoints of the In(1)w (m4) rearrangement
title_short Exact breakpoints of the In(1)w (m4) rearrangement
title_sort exact breakpoints of the in(1)w (m4) rearrangement
topic Data Updates
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9315410/
https://www.ncbi.nlm.nih.gov/pubmed/35903781
http://dx.doi.org/10.17912/micropub.biology.000608
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