Cargando…

The Transcription Factor MYB37 Positively Regulates Photosynthetic Inhibition and Oxidative Damage in Arabidopsis Leaves Under Salt Stress

MYB transcription factors (TFs) mediate plant responses and defenses to biotic and abiotic stresses. The effects of overexpression of MYB37, an R2R3 MYB subgroup 14 transcription factors in Arabidopsis thaliana, on chlorophyll content, chlorophyll fluorescence parameters, reactive oxygen species (RO...

Descripción completa

Detalles Bibliográficos
Autores principales: Li, Yuanyuan, Tian, Bei, Wang, Yue, Wang, Jiechen, Zhang, Hongbo, Wang, Lu, Sun, Guangyu, Yu, Yongtao, Zhang, Huihui
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9315438/
https://www.ncbi.nlm.nih.gov/pubmed/35903240
http://dx.doi.org/10.3389/fpls.2022.943153
_version_ 1784754562048983040
author Li, Yuanyuan
Tian, Bei
Wang, Yue
Wang, Jiechen
Zhang, Hongbo
Wang, Lu
Sun, Guangyu
Yu, Yongtao
Zhang, Huihui
author_facet Li, Yuanyuan
Tian, Bei
Wang, Yue
Wang, Jiechen
Zhang, Hongbo
Wang, Lu
Sun, Guangyu
Yu, Yongtao
Zhang, Huihui
author_sort Li, Yuanyuan
collection PubMed
description MYB transcription factors (TFs) mediate plant responses and defenses to biotic and abiotic stresses. The effects of overexpression of MYB37, an R2R3 MYB subgroup 14 transcription factors in Arabidopsis thaliana, on chlorophyll content, chlorophyll fluorescence parameters, reactive oxygen species (ROS) metabolism, and the contents of osmotic regulatory substances were studied under 100 mM NaCl stress. Compared with the wild type (Col-0), MYB37 overexpression significantly alleviated the salt stress symptoms in A. thaliana plants. Chlorophyll a (Chl a) and chlorophyll b (Chl b) contents were significantly decreased in OE-1 and OE-2 than in Col-0. Particularly, the Chl a/b ratio was also higher in OE-1 and OE-2 than in Col-0 under NaCl stress. However, MYB37 overexpression alleviated the degradation of chlorophyll, especially Chl a. Salt stress inhibited the activities of PSII and PSI in Arabidopsis leaves, but did not affect the activity of PSII electron donor side oxygen-evolving complex (OEC). MYB37 overexpression increased photosynthesis in Arabidopsis by increasing PSII and PSI activities. MYB37 overexpression also promoted the transfer of electrons from Q(A) to Q(B) on the PSII receptor side of Arabidopsis under NaCl stress. Additionally, MYB37 overexpression increased Y(II) and Y(NPQ) of Arabidopsis under NaCl stress and decreased Y(NO). These results indicate that MYB37 overexpression increases PSII activity and regulates the proportion of energy dissipation in Arabidopsis leaves under NaCl stress, thus decreasing the proportion of inactivated reaction centers. Salt stress causes excess electrons and energy in the photosynthetic electron transport chain of Arabidopsis leaves, resulting in the release of reactive oxygen species (ROS), such as superoxide anion and hydrogen peroxide, leading to oxidative damage. Nevertheless, MYB37 overexpression reduced accumulation of malondialdehyde in Arabidopsis leaves under NaCl stress and alleviated the degree of membrane lipid peroxidation caused by ROS. Salt stress also enhanced the accumulation of soluble sugar (SS) and proline (Pro) in Arabidopsis leaves, thus reducing salt stress damage to plants. Salt stress also degraded soluble protein (SP). Furthermore, the accumulation of osmoregulation substances SS and Pro in OE-1 and OE-2 was not different from that in Col-0 since MYB37 overexpression in Arabidopsis OE-1, and OE-2 did not significantly affect plants under NaCl stress. However, SP content was significantly higher in OE-1 and OE-2 than in Col-0. These results indicate that MYB37 overexpression can alleviate the degradation of Arabidopsis proteins under NaCl stress, promote plant growth and improve salt tolerance.
format Online
Article
Text
id pubmed-9315438
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher Frontiers Media S.A.
record_format MEDLINE/PubMed
spelling pubmed-93154382022-07-27 The Transcription Factor MYB37 Positively Regulates Photosynthetic Inhibition and Oxidative Damage in Arabidopsis Leaves Under Salt Stress Li, Yuanyuan Tian, Bei Wang, Yue Wang, Jiechen Zhang, Hongbo Wang, Lu Sun, Guangyu Yu, Yongtao Zhang, Huihui Front Plant Sci Plant Science MYB transcription factors (TFs) mediate plant responses and defenses to biotic and abiotic stresses. The effects of overexpression of MYB37, an R2R3 MYB subgroup 14 transcription factors in Arabidopsis thaliana, on chlorophyll content, chlorophyll fluorescence parameters, reactive oxygen species (ROS) metabolism, and the contents of osmotic regulatory substances were studied under 100 mM NaCl stress. Compared with the wild type (Col-0), MYB37 overexpression significantly alleviated the salt stress symptoms in A. thaliana plants. Chlorophyll a (Chl a) and chlorophyll b (Chl b) contents were significantly decreased in OE-1 and OE-2 than in Col-0. Particularly, the Chl a/b ratio was also higher in OE-1 and OE-2 than in Col-0 under NaCl stress. However, MYB37 overexpression alleviated the degradation of chlorophyll, especially Chl a. Salt stress inhibited the activities of PSII and PSI in Arabidopsis leaves, but did not affect the activity of PSII electron donor side oxygen-evolving complex (OEC). MYB37 overexpression increased photosynthesis in Arabidopsis by increasing PSII and PSI activities. MYB37 overexpression also promoted the transfer of electrons from Q(A) to Q(B) on the PSII receptor side of Arabidopsis under NaCl stress. Additionally, MYB37 overexpression increased Y(II) and Y(NPQ) of Arabidopsis under NaCl stress and decreased Y(NO). These results indicate that MYB37 overexpression increases PSII activity and regulates the proportion of energy dissipation in Arabidopsis leaves under NaCl stress, thus decreasing the proportion of inactivated reaction centers. Salt stress causes excess electrons and energy in the photosynthetic electron transport chain of Arabidopsis leaves, resulting in the release of reactive oxygen species (ROS), such as superoxide anion and hydrogen peroxide, leading to oxidative damage. Nevertheless, MYB37 overexpression reduced accumulation of malondialdehyde in Arabidopsis leaves under NaCl stress and alleviated the degree of membrane lipid peroxidation caused by ROS. Salt stress also enhanced the accumulation of soluble sugar (SS) and proline (Pro) in Arabidopsis leaves, thus reducing salt stress damage to plants. Salt stress also degraded soluble protein (SP). Furthermore, the accumulation of osmoregulation substances SS and Pro in OE-1 and OE-2 was not different from that in Col-0 since MYB37 overexpression in Arabidopsis OE-1, and OE-2 did not significantly affect plants under NaCl stress. However, SP content was significantly higher in OE-1 and OE-2 than in Col-0. These results indicate that MYB37 overexpression can alleviate the degradation of Arabidopsis proteins under NaCl stress, promote plant growth and improve salt tolerance. Frontiers Media S.A. 2022-07-12 /pmc/articles/PMC9315438/ /pubmed/35903240 http://dx.doi.org/10.3389/fpls.2022.943153 Text en Copyright © 2022 Li, Tian, Wang, Wang, Zhang, Wang, Sun, Yu and Zhang. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Plant Science
Li, Yuanyuan
Tian, Bei
Wang, Yue
Wang, Jiechen
Zhang, Hongbo
Wang, Lu
Sun, Guangyu
Yu, Yongtao
Zhang, Huihui
The Transcription Factor MYB37 Positively Regulates Photosynthetic Inhibition and Oxidative Damage in Arabidopsis Leaves Under Salt Stress
title The Transcription Factor MYB37 Positively Regulates Photosynthetic Inhibition and Oxidative Damage in Arabidopsis Leaves Under Salt Stress
title_full The Transcription Factor MYB37 Positively Regulates Photosynthetic Inhibition and Oxidative Damage in Arabidopsis Leaves Under Salt Stress
title_fullStr The Transcription Factor MYB37 Positively Regulates Photosynthetic Inhibition and Oxidative Damage in Arabidopsis Leaves Under Salt Stress
title_full_unstemmed The Transcription Factor MYB37 Positively Regulates Photosynthetic Inhibition and Oxidative Damage in Arabidopsis Leaves Under Salt Stress
title_short The Transcription Factor MYB37 Positively Regulates Photosynthetic Inhibition and Oxidative Damage in Arabidopsis Leaves Under Salt Stress
title_sort transcription factor myb37 positively regulates photosynthetic inhibition and oxidative damage in arabidopsis leaves under salt stress
topic Plant Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9315438/
https://www.ncbi.nlm.nih.gov/pubmed/35903240
http://dx.doi.org/10.3389/fpls.2022.943153
work_keys_str_mv AT liyuanyuan thetranscriptionfactormyb37positivelyregulatesphotosyntheticinhibitionandoxidativedamageinarabidopsisleavesundersaltstress
AT tianbei thetranscriptionfactormyb37positivelyregulatesphotosyntheticinhibitionandoxidativedamageinarabidopsisleavesundersaltstress
AT wangyue thetranscriptionfactormyb37positivelyregulatesphotosyntheticinhibitionandoxidativedamageinarabidopsisleavesundersaltstress
AT wangjiechen thetranscriptionfactormyb37positivelyregulatesphotosyntheticinhibitionandoxidativedamageinarabidopsisleavesundersaltstress
AT zhanghongbo thetranscriptionfactormyb37positivelyregulatesphotosyntheticinhibitionandoxidativedamageinarabidopsisleavesundersaltstress
AT wanglu thetranscriptionfactormyb37positivelyregulatesphotosyntheticinhibitionandoxidativedamageinarabidopsisleavesundersaltstress
AT sunguangyu thetranscriptionfactormyb37positivelyregulatesphotosyntheticinhibitionandoxidativedamageinarabidopsisleavesundersaltstress
AT yuyongtao thetranscriptionfactormyb37positivelyregulatesphotosyntheticinhibitionandoxidativedamageinarabidopsisleavesundersaltstress
AT zhanghuihui thetranscriptionfactormyb37positivelyregulatesphotosyntheticinhibitionandoxidativedamageinarabidopsisleavesundersaltstress
AT liyuanyuan transcriptionfactormyb37positivelyregulatesphotosyntheticinhibitionandoxidativedamageinarabidopsisleavesundersaltstress
AT tianbei transcriptionfactormyb37positivelyregulatesphotosyntheticinhibitionandoxidativedamageinarabidopsisleavesundersaltstress
AT wangyue transcriptionfactormyb37positivelyregulatesphotosyntheticinhibitionandoxidativedamageinarabidopsisleavesundersaltstress
AT wangjiechen transcriptionfactormyb37positivelyregulatesphotosyntheticinhibitionandoxidativedamageinarabidopsisleavesundersaltstress
AT zhanghongbo transcriptionfactormyb37positivelyregulatesphotosyntheticinhibitionandoxidativedamageinarabidopsisleavesundersaltstress
AT wanglu transcriptionfactormyb37positivelyregulatesphotosyntheticinhibitionandoxidativedamageinarabidopsisleavesundersaltstress
AT sunguangyu transcriptionfactormyb37positivelyregulatesphotosyntheticinhibitionandoxidativedamageinarabidopsisleavesundersaltstress
AT yuyongtao transcriptionfactormyb37positivelyregulatesphotosyntheticinhibitionandoxidativedamageinarabidopsisleavesundersaltstress
AT zhanghuihui transcriptionfactormyb37positivelyregulatesphotosyntheticinhibitionandoxidativedamageinarabidopsisleavesundersaltstress