Cargando…

Creating a Functional Biomimetic Cartilage Implant Using Hydrogels Based on Methacrylated Chondroitin Sulfate and Hyaluronic Acid

The load-bearing function of articular cartilage tissue contrasts with the poor load-bearing capacity of most soft hydrogels used for its regeneration. The present study explores whether a hydrogel based on the methacrylated natural polymers chondroitin sulfate (CSMA) and hyaluronic acid (HAMA), inj...

Descripción completa

Detalles Bibliográficos
Autores principales: Schuiringa, Gerke H., Mihajlovic, Marko, van Donkelaar, Corrinus C., Vermonden, Tina, Ito, Keita
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9315485/
https://www.ncbi.nlm.nih.gov/pubmed/35877542
http://dx.doi.org/10.3390/gels8070457
_version_ 1784754573492092928
author Schuiringa, Gerke H.
Mihajlovic, Marko
van Donkelaar, Corrinus C.
Vermonden, Tina
Ito, Keita
author_facet Schuiringa, Gerke H.
Mihajlovic, Marko
van Donkelaar, Corrinus C.
Vermonden, Tina
Ito, Keita
author_sort Schuiringa, Gerke H.
collection PubMed
description The load-bearing function of articular cartilage tissue contrasts with the poor load-bearing capacity of most soft hydrogels used for its regeneration. The present study explores whether a hydrogel based on the methacrylated natural polymers chondroitin sulfate (CSMA) and hyaluronic acid (HAMA), injected into warp-knitted spacer fabrics, could be used to create a biomimetic construct with cartilage-like mechanical properties. The swelling ratio of the combined CSMA/HAMA hydrogels in the first 20 days was higher for hydrogels with a higher CSMA concentration, and these hydrogels also degraded quicker, whereas those with a 1.33 wt% of HAMA were stable for more than 120 days. When confined by a polyamide 6 (PA6) spacer fabric, the volumetric swelling of the combined CSMA/HAMA gels (10 wt%, 6.5 × CSMA:HAMA ratio) was reduced by ~53%. Both the apparent peak and the equilibrium modulus significantly increased in the PA6-restricted constructs compared to the free-swelling hydrogels after 28 days of swelling, and no significant differences in the moduli and time constant compared to native bovine cartilage were observed. Moreover, the cell viability in the CSMA/HAMA PA6 constructs was comparable to that in gelatin–methacrylamide (GelMA) PA6 constructs at one day after polymerization. These results suggest that using a HydroSpacer construct with an extracellular matrix (ECM)-like biopolymer-based hydrogel is a promising approach for mimicking the load-bearing properties of native cartilage.
format Online
Article
Text
id pubmed-9315485
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-93154852022-07-27 Creating a Functional Biomimetic Cartilage Implant Using Hydrogels Based on Methacrylated Chondroitin Sulfate and Hyaluronic Acid Schuiringa, Gerke H. Mihajlovic, Marko van Donkelaar, Corrinus C. Vermonden, Tina Ito, Keita Gels Article The load-bearing function of articular cartilage tissue contrasts with the poor load-bearing capacity of most soft hydrogels used for its regeneration. The present study explores whether a hydrogel based on the methacrylated natural polymers chondroitin sulfate (CSMA) and hyaluronic acid (HAMA), injected into warp-knitted spacer fabrics, could be used to create a biomimetic construct with cartilage-like mechanical properties. The swelling ratio of the combined CSMA/HAMA hydrogels in the first 20 days was higher for hydrogels with a higher CSMA concentration, and these hydrogels also degraded quicker, whereas those with a 1.33 wt% of HAMA were stable for more than 120 days. When confined by a polyamide 6 (PA6) spacer fabric, the volumetric swelling of the combined CSMA/HAMA gels (10 wt%, 6.5 × CSMA:HAMA ratio) was reduced by ~53%. Both the apparent peak and the equilibrium modulus significantly increased in the PA6-restricted constructs compared to the free-swelling hydrogels after 28 days of swelling, and no significant differences in the moduli and time constant compared to native bovine cartilage were observed. Moreover, the cell viability in the CSMA/HAMA PA6 constructs was comparable to that in gelatin–methacrylamide (GelMA) PA6 constructs at one day after polymerization. These results suggest that using a HydroSpacer construct with an extracellular matrix (ECM)-like biopolymer-based hydrogel is a promising approach for mimicking the load-bearing properties of native cartilage. MDPI 2022-07-21 /pmc/articles/PMC9315485/ /pubmed/35877542 http://dx.doi.org/10.3390/gels8070457 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Schuiringa, Gerke H.
Mihajlovic, Marko
van Donkelaar, Corrinus C.
Vermonden, Tina
Ito, Keita
Creating a Functional Biomimetic Cartilage Implant Using Hydrogels Based on Methacrylated Chondroitin Sulfate and Hyaluronic Acid
title Creating a Functional Biomimetic Cartilage Implant Using Hydrogels Based on Methacrylated Chondroitin Sulfate and Hyaluronic Acid
title_full Creating a Functional Biomimetic Cartilage Implant Using Hydrogels Based on Methacrylated Chondroitin Sulfate and Hyaluronic Acid
title_fullStr Creating a Functional Biomimetic Cartilage Implant Using Hydrogels Based on Methacrylated Chondroitin Sulfate and Hyaluronic Acid
title_full_unstemmed Creating a Functional Biomimetic Cartilage Implant Using Hydrogels Based on Methacrylated Chondroitin Sulfate and Hyaluronic Acid
title_short Creating a Functional Biomimetic Cartilage Implant Using Hydrogels Based on Methacrylated Chondroitin Sulfate and Hyaluronic Acid
title_sort creating a functional biomimetic cartilage implant using hydrogels based on methacrylated chondroitin sulfate and hyaluronic acid
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9315485/
https://www.ncbi.nlm.nih.gov/pubmed/35877542
http://dx.doi.org/10.3390/gels8070457
work_keys_str_mv AT schuiringagerkeh creatingafunctionalbiomimeticcartilageimplantusinghydrogelsbasedonmethacrylatedchondroitinsulfateandhyaluronicacid
AT mihajlovicmarko creatingafunctionalbiomimeticcartilageimplantusinghydrogelsbasedonmethacrylatedchondroitinsulfateandhyaluronicacid
AT vandonkelaarcorrinusc creatingafunctionalbiomimeticcartilageimplantusinghydrogelsbasedonmethacrylatedchondroitinsulfateandhyaluronicacid
AT vermondentina creatingafunctionalbiomimeticcartilageimplantusinghydrogelsbasedonmethacrylatedchondroitinsulfateandhyaluronicacid
AT itokeita creatingafunctionalbiomimeticcartilageimplantusinghydrogelsbasedonmethacrylatedchondroitinsulfateandhyaluronicacid