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How Different Dietary Methionine Sources Could Modulate the Hepatic Metabolism in Rainbow Trout?

In aquafeeds in which plant proteins are used to replace fishmeal, exogenous methionine (Met) sources are demanded to balance the amino acid composition of diets and meet the metabolic fish requirements. Nonetheless, since different synthetic Met sources are commercially available, it is important t...

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Autores principales: Ceccotti, Chiara, Biasato, Ilaria, Gasco, Laura, Caimi, Christian, Bellezza Oddon, Sara, Rimoldi, Simona, Brambilla, Fabio, Terova, Genciana
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9315512/
https://www.ncbi.nlm.nih.gov/pubmed/35877447
http://dx.doi.org/10.3390/cimb44070223
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author Ceccotti, Chiara
Biasato, Ilaria
Gasco, Laura
Caimi, Christian
Bellezza Oddon, Sara
Rimoldi, Simona
Brambilla, Fabio
Terova, Genciana
author_facet Ceccotti, Chiara
Biasato, Ilaria
Gasco, Laura
Caimi, Christian
Bellezza Oddon, Sara
Rimoldi, Simona
Brambilla, Fabio
Terova, Genciana
author_sort Ceccotti, Chiara
collection PubMed
description In aquafeeds in which plant proteins are used to replace fishmeal, exogenous methionine (Met) sources are demanded to balance the amino acid composition of diets and meet the metabolic fish requirements. Nonetheless, since different synthetic Met sources are commercially available, it is important to determine their bioavailability and efficacy. To address this issue, we conducted a two-month feeding trial with rainbow trout (Oncorhynchus mykiss), which were fed diets supplemented with five different forms of Met: Met-Met, L-Met, HMTBa, DL-Met, and Co DL-Met. No differences in growth performance were found in trout fed with different Met forms, but changes in the whole-body composition were found. In particular, Met-Met and L-Met promoted a significant body lipid reduction, whereas the protein retention was significantly increased in fish fed with HMTBa and Co DL-Met. The latter affected the hepatic Met metabolism promoting the trans-sulfuration pathway through the upregulation of CBS gene expression. Similarly, the L-Met enhanced the remethylation pathway through an increase in BHMT gene expression to maintain the cellular demand for Met. Altogether, our findings suggest an optimal dietary intake of all tested Met sources with similar promoting effects on fish growth and hepatic Met metabolism. Nevertheless, the mechanisms underlying these effects warrant further investigation.
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spelling pubmed-93155122022-07-27 How Different Dietary Methionine Sources Could Modulate the Hepatic Metabolism in Rainbow Trout? Ceccotti, Chiara Biasato, Ilaria Gasco, Laura Caimi, Christian Bellezza Oddon, Sara Rimoldi, Simona Brambilla, Fabio Terova, Genciana Curr Issues Mol Biol Article In aquafeeds in which plant proteins are used to replace fishmeal, exogenous methionine (Met) sources are demanded to balance the amino acid composition of diets and meet the metabolic fish requirements. Nonetheless, since different synthetic Met sources are commercially available, it is important to determine their bioavailability and efficacy. To address this issue, we conducted a two-month feeding trial with rainbow trout (Oncorhynchus mykiss), which were fed diets supplemented with five different forms of Met: Met-Met, L-Met, HMTBa, DL-Met, and Co DL-Met. No differences in growth performance were found in trout fed with different Met forms, but changes in the whole-body composition were found. In particular, Met-Met and L-Met promoted a significant body lipid reduction, whereas the protein retention was significantly increased in fish fed with HMTBa and Co DL-Met. The latter affected the hepatic Met metabolism promoting the trans-sulfuration pathway through the upregulation of CBS gene expression. Similarly, the L-Met enhanced the remethylation pathway through an increase in BHMT gene expression to maintain the cellular demand for Met. Altogether, our findings suggest an optimal dietary intake of all tested Met sources with similar promoting effects on fish growth and hepatic Met metabolism. Nevertheless, the mechanisms underlying these effects warrant further investigation. MDPI 2022-07-19 /pmc/articles/PMC9315512/ /pubmed/35877447 http://dx.doi.org/10.3390/cimb44070223 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Ceccotti, Chiara
Biasato, Ilaria
Gasco, Laura
Caimi, Christian
Bellezza Oddon, Sara
Rimoldi, Simona
Brambilla, Fabio
Terova, Genciana
How Different Dietary Methionine Sources Could Modulate the Hepatic Metabolism in Rainbow Trout?
title How Different Dietary Methionine Sources Could Modulate the Hepatic Metabolism in Rainbow Trout?
title_full How Different Dietary Methionine Sources Could Modulate the Hepatic Metabolism in Rainbow Trout?
title_fullStr How Different Dietary Methionine Sources Could Modulate the Hepatic Metabolism in Rainbow Trout?
title_full_unstemmed How Different Dietary Methionine Sources Could Modulate the Hepatic Metabolism in Rainbow Trout?
title_short How Different Dietary Methionine Sources Could Modulate the Hepatic Metabolism in Rainbow Trout?
title_sort how different dietary methionine sources could modulate the hepatic metabolism in rainbow trout?
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9315512/
https://www.ncbi.nlm.nih.gov/pubmed/35877447
http://dx.doi.org/10.3390/cimb44070223
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