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Novel Animal Model of Spontaneous Cerebral Petechial Hemorrhage Using Focused Ultrasound in Rats
Background and Objectives: Petechial cerebral hemorrhages can be caused by various factors, such as traumas, cerebral infarctions, and aging, and is related to the disruption of the blood–brain barrier or the cellular damage of blood vessels. However, there is no animal model that recapitulates cere...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9315537/ https://www.ncbi.nlm.nih.gov/pubmed/35888600 http://dx.doi.org/10.3390/medicina58070881 |
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author | Yoon, Sang-Youl Han, Mun Lee, Chaejin Lee, Eun-Hee Kim, Moonsik Kim, Kyoung-Tae Hwang, Jeong-Hyun Na, Sungdae Park, Juyoung Park, Ki-Su |
author_facet | Yoon, Sang-Youl Han, Mun Lee, Chaejin Lee, Eun-Hee Kim, Moonsik Kim, Kyoung-Tae Hwang, Jeong-Hyun Na, Sungdae Park, Juyoung Park, Ki-Su |
author_sort | Yoon, Sang-Youl |
collection | PubMed |
description | Background and Objectives: Petechial cerebral hemorrhages can be caused by various factors, such as traumas, cerebral infarctions, and aging, and is related to the disruption of the blood–brain barrier or the cellular damage of blood vessels. However, there is no animal model that recapitulates cerebral petechial hemorrhages. Materials and Methods: Here, we implemented a petechial hemorrhage using a novel technology, i.e., microbubble-assisted focused ultrasound (MB + FUS). Results: This method increases the permeability of the blood–brain barrier by directly applying mechanical force to the vascular endothelial cells through cavitation of the microbubbles. Microbubble-enhanced cavitation has the advantage of controlling the degree and location of petechial hemorrhages. Conclusions: We thus generated a preclinical rat model using noninvasive focal MB + FUS. This method is histologically similar to actual petechial hemorrhages of the brain and allows the achievement of a physiologically resembling petechial hemorrhage. In the future, this method shall be considered as a useful animal model for studying the pathophysiology and treatment of petechial cerebral hemorrhages. |
format | Online Article Text |
id | pubmed-9315537 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-93155372022-07-27 Novel Animal Model of Spontaneous Cerebral Petechial Hemorrhage Using Focused Ultrasound in Rats Yoon, Sang-Youl Han, Mun Lee, Chaejin Lee, Eun-Hee Kim, Moonsik Kim, Kyoung-Tae Hwang, Jeong-Hyun Na, Sungdae Park, Juyoung Park, Ki-Su Medicina (Kaunas) Article Background and Objectives: Petechial cerebral hemorrhages can be caused by various factors, such as traumas, cerebral infarctions, and aging, and is related to the disruption of the blood–brain barrier or the cellular damage of blood vessels. However, there is no animal model that recapitulates cerebral petechial hemorrhages. Materials and Methods: Here, we implemented a petechial hemorrhage using a novel technology, i.e., microbubble-assisted focused ultrasound (MB + FUS). Results: This method increases the permeability of the blood–brain barrier by directly applying mechanical force to the vascular endothelial cells through cavitation of the microbubbles. Microbubble-enhanced cavitation has the advantage of controlling the degree and location of petechial hemorrhages. Conclusions: We thus generated a preclinical rat model using noninvasive focal MB + FUS. This method is histologically similar to actual petechial hemorrhages of the brain and allows the achievement of a physiologically resembling petechial hemorrhage. In the future, this method shall be considered as a useful animal model for studying the pathophysiology and treatment of petechial cerebral hemorrhages. MDPI 2022-06-30 /pmc/articles/PMC9315537/ /pubmed/35888600 http://dx.doi.org/10.3390/medicina58070881 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Yoon, Sang-Youl Han, Mun Lee, Chaejin Lee, Eun-Hee Kim, Moonsik Kim, Kyoung-Tae Hwang, Jeong-Hyun Na, Sungdae Park, Juyoung Park, Ki-Su Novel Animal Model of Spontaneous Cerebral Petechial Hemorrhage Using Focused Ultrasound in Rats |
title | Novel Animal Model of Spontaneous Cerebral Petechial Hemorrhage Using Focused Ultrasound in Rats |
title_full | Novel Animal Model of Spontaneous Cerebral Petechial Hemorrhage Using Focused Ultrasound in Rats |
title_fullStr | Novel Animal Model of Spontaneous Cerebral Petechial Hemorrhage Using Focused Ultrasound in Rats |
title_full_unstemmed | Novel Animal Model of Spontaneous Cerebral Petechial Hemorrhage Using Focused Ultrasound in Rats |
title_short | Novel Animal Model of Spontaneous Cerebral Petechial Hemorrhage Using Focused Ultrasound in Rats |
title_sort | novel animal model of spontaneous cerebral petechial hemorrhage using focused ultrasound in rats |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9315537/ https://www.ncbi.nlm.nih.gov/pubmed/35888600 http://dx.doi.org/10.3390/medicina58070881 |
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