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Novel Animal Model of Spontaneous Cerebral Petechial Hemorrhage Using Focused Ultrasound in Rats

Background and Objectives: Petechial cerebral hemorrhages can be caused by various factors, such as traumas, cerebral infarctions, and aging, and is related to the disruption of the blood–brain barrier or the cellular damage of blood vessels. However, there is no animal model that recapitulates cere...

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Autores principales: Yoon, Sang-Youl, Han, Mun, Lee, Chaejin, Lee, Eun-Hee, Kim, Moonsik, Kim, Kyoung-Tae, Hwang, Jeong-Hyun, Na, Sungdae, Park, Juyoung, Park, Ki-Su
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9315537/
https://www.ncbi.nlm.nih.gov/pubmed/35888600
http://dx.doi.org/10.3390/medicina58070881
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author Yoon, Sang-Youl
Han, Mun
Lee, Chaejin
Lee, Eun-Hee
Kim, Moonsik
Kim, Kyoung-Tae
Hwang, Jeong-Hyun
Na, Sungdae
Park, Juyoung
Park, Ki-Su
author_facet Yoon, Sang-Youl
Han, Mun
Lee, Chaejin
Lee, Eun-Hee
Kim, Moonsik
Kim, Kyoung-Tae
Hwang, Jeong-Hyun
Na, Sungdae
Park, Juyoung
Park, Ki-Su
author_sort Yoon, Sang-Youl
collection PubMed
description Background and Objectives: Petechial cerebral hemorrhages can be caused by various factors, such as traumas, cerebral infarctions, and aging, and is related to the disruption of the blood–brain barrier or the cellular damage of blood vessels. However, there is no animal model that recapitulates cerebral petechial hemorrhages. Materials and Methods: Here, we implemented a petechial hemorrhage using a novel technology, i.e., microbubble-assisted focused ultrasound (MB + FUS). Results: This method increases the permeability of the blood–brain barrier by directly applying mechanical force to the vascular endothelial cells through cavitation of the microbubbles. Microbubble-enhanced cavitation has the advantage of controlling the degree and location of petechial hemorrhages. Conclusions: We thus generated a preclinical rat model using noninvasive focal MB + FUS. This method is histologically similar to actual petechial hemorrhages of the brain and allows the achievement of a physiologically resembling petechial hemorrhage. In the future, this method shall be considered as a useful animal model for studying the pathophysiology and treatment of petechial cerebral hemorrhages.
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spelling pubmed-93155372022-07-27 Novel Animal Model of Spontaneous Cerebral Petechial Hemorrhage Using Focused Ultrasound in Rats Yoon, Sang-Youl Han, Mun Lee, Chaejin Lee, Eun-Hee Kim, Moonsik Kim, Kyoung-Tae Hwang, Jeong-Hyun Na, Sungdae Park, Juyoung Park, Ki-Su Medicina (Kaunas) Article Background and Objectives: Petechial cerebral hemorrhages can be caused by various factors, such as traumas, cerebral infarctions, and aging, and is related to the disruption of the blood–brain barrier or the cellular damage of blood vessels. However, there is no animal model that recapitulates cerebral petechial hemorrhages. Materials and Methods: Here, we implemented a petechial hemorrhage using a novel technology, i.e., microbubble-assisted focused ultrasound (MB + FUS). Results: This method increases the permeability of the blood–brain barrier by directly applying mechanical force to the vascular endothelial cells through cavitation of the microbubbles. Microbubble-enhanced cavitation has the advantage of controlling the degree and location of petechial hemorrhages. Conclusions: We thus generated a preclinical rat model using noninvasive focal MB + FUS. This method is histologically similar to actual petechial hemorrhages of the brain and allows the achievement of a physiologically resembling petechial hemorrhage. In the future, this method shall be considered as a useful animal model for studying the pathophysiology and treatment of petechial cerebral hemorrhages. MDPI 2022-06-30 /pmc/articles/PMC9315537/ /pubmed/35888600 http://dx.doi.org/10.3390/medicina58070881 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Yoon, Sang-Youl
Han, Mun
Lee, Chaejin
Lee, Eun-Hee
Kim, Moonsik
Kim, Kyoung-Tae
Hwang, Jeong-Hyun
Na, Sungdae
Park, Juyoung
Park, Ki-Su
Novel Animal Model of Spontaneous Cerebral Petechial Hemorrhage Using Focused Ultrasound in Rats
title Novel Animal Model of Spontaneous Cerebral Petechial Hemorrhage Using Focused Ultrasound in Rats
title_full Novel Animal Model of Spontaneous Cerebral Petechial Hemorrhage Using Focused Ultrasound in Rats
title_fullStr Novel Animal Model of Spontaneous Cerebral Petechial Hemorrhage Using Focused Ultrasound in Rats
title_full_unstemmed Novel Animal Model of Spontaneous Cerebral Petechial Hemorrhage Using Focused Ultrasound in Rats
title_short Novel Animal Model of Spontaneous Cerebral Petechial Hemorrhage Using Focused Ultrasound in Rats
title_sort novel animal model of spontaneous cerebral petechial hemorrhage using focused ultrasound in rats
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9315537/
https://www.ncbi.nlm.nih.gov/pubmed/35888600
http://dx.doi.org/10.3390/medicina58070881
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