Resistance to Extreme Stresses by a Newly Discovered Japanese Tardigrade Species, Macrobiotus kyoukenus (Eutardigrada, Macrobiotidae)

SIMPLE SUMMARY: Tardigrades are small micrometazoans able to resist several environmental stresses in any stage of their life cycle. The integrated molecular and morphological analysis of tardigrade specimens collected in Tsukuba (Japan) revealed that this population represents a new species, Macrobiotus kyoukenus sp. nov., belonging to the genus Macrobiotus, one of the most speciose and widespread water bear taxon. The stress resistance capabilities of M. kyoukenus sp. nov. have been tested by submitting animals to extreme desiccation, rapid freezing, and high levels of ultraviolet radiations (UVB and UVC). Animals were able to survive desiccation and freezing, and both hydrated and desiccated animals showed a high tolerance to increasing UV radiations. Overall, our findings contribute to the discovery of a larger tardigrade biodiversity in Japan, and the tolerance capabilities of M. kyoukenus sp. nov. show that this new species could become an emerging model for stress resistance studies. ABSTRACT: Tardigrades are small micrometazoans able to resist several environmental stresses in any stage of their life cycle. An integrated analysis of tardigrade specimens collected in Tsukuba (Japan) revealed a peculiar morphology and a new sensory field in the cloaca. Molecular taxonomy and phylogenetic analysis on different genes (COI, ITS2, 18S and 28S) confirmed that this population is a new species, Macrobiotus kyoukenus sp. nov., belonging to the widespread Macrobiotus hufelandi group. The stress resistance capabilities of M. kyoukenus sp. nov. have been tested by submitting animals to extreme desiccation, rapid freezing, and high levels of ultraviolet radiations (UVB and UVC). Animals were able to survive desiccation (survivorship 95.71 ± 7.07%) and freezing up to −80 °C (82.33 ± 17.11%). Both hydrated and desiccated animals showed a high tolerance to increasing UV radiations: hydrated animals survived to doses up to 152.22 kJ m(−2) (UVB) and up to 15.00 kJ m(−2) (UVC), while desiccated specimens persisted to radiations up to 165.12 kJ m(−2) (UVB) and up to 35.00 kJ m(−2) (UVC). Present data contribute to the discovery of a larger tardigrade biodiversity in Japan, and the tolerance capabilities of M. kyoukenus sp. nov. show that it could become a new emerging model for stress resistance studies.