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Recombinant Muscovy Duck Parvovirus Led to Ileac Damage in Muscovy Ducklings
Waterfowl parvovirus (WPFs) has multiple effects on the intestinal tract, but the effects of recombinant Muscovy duck parvovirus (rMDPV) have not been elucidated. In this study, 48 one-day-old Muscovy ducklings were divided into an infected group and a control group. Plasma and ileal samples were co...
Autores principales: | , , , , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9315717/ https://www.ncbi.nlm.nih.gov/pubmed/35891451 http://dx.doi.org/10.3390/v14071471 |
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author | He, Jiahui Zhang, Yukun Hu, Zezhong Zhang, Luxuan Shao, Guanming Xie, Zi Nie, Yu Li, Wenxue Li, Yajuan Chen, Liyi Huang, Benli Chu, Fengsheng Feng, Keyu Lin, Wencheng Li, Hongxin Chen, Weiguo Zhang, Xinheng Xie, Qingmei |
author_facet | He, Jiahui Zhang, Yukun Hu, Zezhong Zhang, Luxuan Shao, Guanming Xie, Zi Nie, Yu Li, Wenxue Li, Yajuan Chen, Liyi Huang, Benli Chu, Fengsheng Feng, Keyu Lin, Wencheng Li, Hongxin Chen, Weiguo Zhang, Xinheng Xie, Qingmei |
author_sort | He, Jiahui |
collection | PubMed |
description | Waterfowl parvovirus (WPFs) has multiple effects on the intestinal tract, but the effects of recombinant Muscovy duck parvovirus (rMDPV) have not been elucidated. In this study, 48 one-day-old Muscovy ducklings were divided into an infected group and a control group. Plasma and ileal samples were collected from both groups at 2, 4, 6, and 8 days post-infection (dpi), both six ducklings at a time. Next, we analyzed the genomic sequence of the rMDPV strain. Results showed that the ileal villus structure was destroyed seriously at 4, 6, 8 dpi, and the expression of ZO-1, Occludin, and Claudin-1 decreased at 4, 6 dpi; 4, 6, 8 dpi; and 2, 6 dpi, respectively. Intestinal cytokines IFN-α, IL-1β and IL-6 increased at 6 dpi; 8 dpi; and 6, 8 dpi, respectively, whereas IL-2 decreased at 6, 8 dpi. The diversity of ileal flora increased significantly at 4 dpi and decreased at 8 dpi. The bacteria Ochrobactrum and Enterococcus increased and decreased at 4, 8 dpi; 2, 4 dpi, respectively. Plasma MDA increased at 2 dpi, SOD, CAT, and T-AOC decreased at 2, 4, 8 dpi; 4, 8 dpi; and 4, 6, 8 dpi, respectively. These results suggest that rMDPV infection led to early intestinal barrier dysfunction, inflammation, ileac microbiota disruption, and oxidative stress. |
format | Online Article Text |
id | pubmed-9315717 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-93157172022-07-27 Recombinant Muscovy Duck Parvovirus Led to Ileac Damage in Muscovy Ducklings He, Jiahui Zhang, Yukun Hu, Zezhong Zhang, Luxuan Shao, Guanming Xie, Zi Nie, Yu Li, Wenxue Li, Yajuan Chen, Liyi Huang, Benli Chu, Fengsheng Feng, Keyu Lin, Wencheng Li, Hongxin Chen, Weiguo Zhang, Xinheng Xie, Qingmei Viruses Article Waterfowl parvovirus (WPFs) has multiple effects on the intestinal tract, but the effects of recombinant Muscovy duck parvovirus (rMDPV) have not been elucidated. In this study, 48 one-day-old Muscovy ducklings were divided into an infected group and a control group. Plasma and ileal samples were collected from both groups at 2, 4, 6, and 8 days post-infection (dpi), both six ducklings at a time. Next, we analyzed the genomic sequence of the rMDPV strain. Results showed that the ileal villus structure was destroyed seriously at 4, 6, 8 dpi, and the expression of ZO-1, Occludin, and Claudin-1 decreased at 4, 6 dpi; 4, 6, 8 dpi; and 2, 6 dpi, respectively. Intestinal cytokines IFN-α, IL-1β and IL-6 increased at 6 dpi; 8 dpi; and 6, 8 dpi, respectively, whereas IL-2 decreased at 6, 8 dpi. The diversity of ileal flora increased significantly at 4 dpi and decreased at 8 dpi. The bacteria Ochrobactrum and Enterococcus increased and decreased at 4, 8 dpi; 2, 4 dpi, respectively. Plasma MDA increased at 2 dpi, SOD, CAT, and T-AOC decreased at 2, 4, 8 dpi; 4, 8 dpi; and 4, 6, 8 dpi, respectively. These results suggest that rMDPV infection led to early intestinal barrier dysfunction, inflammation, ileac microbiota disruption, and oxidative stress. MDPI 2022-07-03 /pmc/articles/PMC9315717/ /pubmed/35891451 http://dx.doi.org/10.3390/v14071471 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article He, Jiahui Zhang, Yukun Hu, Zezhong Zhang, Luxuan Shao, Guanming Xie, Zi Nie, Yu Li, Wenxue Li, Yajuan Chen, Liyi Huang, Benli Chu, Fengsheng Feng, Keyu Lin, Wencheng Li, Hongxin Chen, Weiguo Zhang, Xinheng Xie, Qingmei Recombinant Muscovy Duck Parvovirus Led to Ileac Damage in Muscovy Ducklings |
title | Recombinant Muscovy Duck Parvovirus Led to Ileac Damage in Muscovy Ducklings |
title_full | Recombinant Muscovy Duck Parvovirus Led to Ileac Damage in Muscovy Ducklings |
title_fullStr | Recombinant Muscovy Duck Parvovirus Led to Ileac Damage in Muscovy Ducklings |
title_full_unstemmed | Recombinant Muscovy Duck Parvovirus Led to Ileac Damage in Muscovy Ducklings |
title_short | Recombinant Muscovy Duck Parvovirus Led to Ileac Damage in Muscovy Ducklings |
title_sort | recombinant muscovy duck parvovirus led to ileac damage in muscovy ducklings |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9315717/ https://www.ncbi.nlm.nih.gov/pubmed/35891451 http://dx.doi.org/10.3390/v14071471 |
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